CAJ | 학술논문

目的探讨肝纤维化环境中移植骨髓基质细胞(BMSCs)在肝内迁移分布规律及其MR示踪的可行性.方法超顺磁性氧化铁(SPIO)及5-溴脱氧尿核苷(Brdu)双标记BMSCs经门静脉移植入大鼠肝纤维化模型,分别于移植后2h、3d、7d、2周行MR扫描,分析不同时间点BMSCs在肝内MR信号和病理组织学特点.结果 (1)标记的BMSCs经门静脉移植术后MR检查见多发结节状低信号灶随时间向肝内分散.(2)含铁血黄素染色及Brdu免疫组织化学染色阳性细胞:移植术后2h主要分布于肝门部门静脉内,移植术后3d主要分布于门静脉小分支、肝窦内、肝小叶中央静脉周围,移植术后7d、2周主要分布于肝损伤较重区和纤维间隔,部分BMSCs与肝细胞紧密连接,形成规则的肝细胞索.各时间点MRI信号改变与组织学所见一致.结论 (1)经门静脉移植BMSCs随时间逐渐向肝内移行,以损伤较重区和纤维间隔分布明显,具有选择性分布特点,对肝损伤具有修复作用；(2)MR信号变化规律在一定程度上反映了移植细胞在肝内的分布、迁移和分化状态.
목적 탐토간섬유화배경중이식골수기질세포(BMSCs)재간내천이분포규률급기MR시종적가행성.방법 초순자성양화철(SPIO)급5-추탈양뇨핵감(Brdu)쌍표기BMSCs경문정맥이식입대서간섬유화모형,분별우이식후2h、3d、7d、2주행MR소묘,분석불동시간점BMSCs재간내MR신호화병리조직학특점.결과 (1)표기적BMSCs경문정맥이식술후MR검사견다발결절상저신호조수시간향간내분산.(2)함철혈황소염색급Brdu면역조직화학염색양성세포:이식술후2h주요분포우간문부문정맥내,이식술후3d주요분포우문정맥소분지、간두내、간소협중앙정맥주위,이식술후7d、2주주요분포우간손상교중구화섬유간격,부분BMSCs여간세포긴밀련접,형성규칙적간세포색.각시간점MRI신호개변여조직학소견일치.결론 (1)경문정맥이식BMSCs수시간축점향간내이행,이손상교중구화섬유간격분포명현,구유선택성분포특점,대간손상구유수복작용；(2)MR신호변화규률재일정정도상반영료이식세포재간내적분포、천이화분화상태.
Objective To explore the recurring patterns of migration and distribution of transplanted bone marrow stromal cells (BMSCs) in rat model with hepatic fibrosis and the feasibility of magnetic resonance (MR) tracing.Methods BMSCs labeled with 5-bromodeoxyuridine (Brdu) and superparamagnetic iron oxide (SPIO) were transplanted into rat model with hepatic fibrosis via portal vein.MR scan was performed at Hour 2,Day 3,Day 7 and Week 2 post-transplantation to analyze the hepatic features of MR signal intensity and pathohistology of BMSCs.Results Multiple hypo-intense lesions appeared in hepatic hilar region at Hour 2 and became smaller with the elapsing time.Hemosiderin and Brdu immunohistochemical stains showed that positive cells were found in portal vein of hepatic porta at Hour 2,small branches of portal vein,sinus hepaticus and around central veins of hepatic lobules at Day 3 and liver parenchyma (esp.in area of lesion) at Day 7 and Week 2.Some of transplanted BMSCs were tightly connected with liver cell to form liver cell cord.The signal intensity changes of MRI corresponded to histological findings at different timepoints.Conclusion The transplanted BMSCs are gradually scattered in whole liver (esp.in lesion area) so that it may help to repair hepatic lesions.And the recurring patterns of MR signal intensity changes reflected the condition of distribution,immigration and differentiation of transplanted cells.