CAJ | 학술논문

目的探讨3-溴丙酮酸(3-BrPA)经肝动脉灌注对兔VX2肝肿瘤转移及荷瘤兔生存时间的影响.方法 18只新西兰大白兔肝左叶种植VX2肿瘤,随机分成3组,每组6只.PBS灌注组:在肿瘤种植14 d后行肝动脉PBS溶液灌注.3-BrPA 7和14 d灌注组:在肿瘤种植7/14 d后行肝动脉3-BrPA溶液灌注.在肿瘤种植28 d后每组处死3只兔,解剖探查有无肝内转移、肾转移、肺转移及腹腔转移.每组剩余的3只兔观察其生存时间并进行比较.结果肿瘤种植28 d后,PBS灌注组均发现肝内及腹腔转移(3/3),肾转移2只(2/3),肺转移2只(2/3).3-BrPA 7 d灌注组实验兔肝内和肺转移各有1只(1/3),未发现有腹腔和肾转移(0/3).3-BrPA14 d灌注组实验兔有2只发现肝内转移(2/3),肺和腹腔转移各有1只(1/3),未发现肾转移(0/3).生存时间比较显示3-BrPA 14 d灌注组实验兔生存时间[(27±5)d]显著长于PBS溶液灌注组[(17±3)d](P=0.041).而3-BrPA 7 d灌注组实验兔生存时间[(42±6)d]显著长于3-BrPA 14 d灌注组实验兔[(27±5)d](P=0.007).结论经肝动脉灌3-BrPA能够有效减少兔VX2肝肿瘤的转移,并可延长移植VX2肝肿瘤兔的生存时间,且灌注时间越早,治疗效果越好.
목적 탐토3-추병동산(3-BrPA)경간동맥관주대토VX2간종류전이급하류토생존시간적영향.방법 18지신서란대백토간좌협충식VX2종류,수궤분성3조,매조6지.PBS관주조:재종류충식14 d후행간동맥PBS용액관주.3-BrPA 7화14 d관주조:재종류충식7/14 d후행간동맥3-BrPA용액관주.재종류충식28 d후매조처사3지토,해부탐사유무간내전이、신전이、폐전이급복강전이.매조잉여적3지토관찰기생존시간병진행비교.결과 종류충식28 d후,PBS관주조균발현간내급복강전이(3/3),신전이2지(2/3),폐전이2지(2/3).3-BrPA 7 d관주조실험토간내화폐전이각유1지(1/3),미발현유복강화신전이(0/3).3-BrPA14 d관주조실험토유2지발현간내전이(2/3),폐화복강전이각유1지(1/3),미발현신전이(0/3).생존시간비교현시3-BrPA 14 d관주조실험토생존시간[(27±5)d]현저장우PBS용액관주조[(17±3)d](P=0.041).이3-BrPA 7 d관주조실험토생존시간[(42±6)d]현저장우3-BrPA 14 d관주조실험토[(27±5)d](P=0.007).결론 경간동맥관3-BrPA능구유효감소토VX2간종류적전이,병가연장이식VX2간종류토적생존시간,차관주시간월조,치료효과월호.
Objective To evaluate the metastasis and survival of an intra-arterial infusion of 3-bromopyruvate (3-BrPA) on hepatic VX2 tumor in rabbits.Methods VX2 tumor was implanted in left lateral lobe of liver of 18 white New Zealand rabbits.The animals were randomized into 3 groups (n =6 each) and underwent an intra-arterial infusion of phosphate-buffered saline or 3-BrPA via hepatic artery at 14 days post-implantation.At 28 days post-implantation,3 rabbits in each group were sacrificed.The abdomen of these rabbits was opened and inspected for metastases.Then the survival of the remaining rabbits was observed.Results At 28 days post-implantation,in PBS group,there were intrahepatic metastasis and abdominal cavity dissemination (n =3),renal metastases (n =2) and lung metastases (n =2) ; in early 3-BrPA infusion group,intrahepatic metastasis (n =2),abdominal cavity dissemination (n =1) and lung metastases (n =1) ; in late 3-BrPA infusion group,intrahepatic metastasis (n =1) and lung metastases (n =1).The survival of the remaining animals was observed.Rabbits in early 3-BrPA infusion group survived significantly longer than those in PBS group [(27 ± 5) vs (17 ± 3) days,P =0.041] ; rabbits in late 3-BrPA infusion group [(42 ± 6) days] survived significantly longer than those in early 3-BrPA infusion group (P =0.007).Conclusion An intra-arterial infusion of 3-BrPA could reduce metastasis and prolong survival in rabbits with hepatic VX2 tumor.The earlier the infusion,the better the outcome.