CAJ | 학술논문

目的观察骨髓浓缩液-PGA支架复合在兔关节软骨修复中对骨髓刺激的作用.方法 30只成年雄性新西兰白兔,麻醉后构建兔的膝关节软骨缺损模型,将所有兔子分为5个治疗组:无治疗组(空白对照)、骨髓刺激(BMS)组、BMS+ PGA支架植入组、BMS+ PGA支架与自体骨髓浓缩液(BMC)复合物植入组和BMS+PGA支架与骨髓干细胞(BME)复合物植入组.通过软骨大体观、HE染色、甲苯胺蓝染色及免疫组化等方法处理,评估骨髓浓缩技术对骨髓刺激增强作用及兔软骨缺损修复情况.结果 BMS后软骨损伤区平均MSC数量与从髂嵴获得后MSC的数量大致相同,(26.3±6.0) MSCs比(27.6±6.5)MSCs.兔软骨缺损区可见明显的新生软骨形成,白色光滑的修复组织填充缺损区,与周围组织整合较好.HE染色见损伤区以非常有序的组织填充事其表面规则,并且与原软骨组织整合较好；甲苯胺蓝染色、Ⅱ型胶原蛋白免疫组化染色较前3组更强、更平滑、更规则,ICRS视觉组织学评分也显著高于前3组,与BME组结果相似.修复效果明显优于前3组.结论骨髓浓缩液-PGA支架复合对骨髓刺激具有增强作用,是一种简易、有效的方法,有望应用于关节软骨的修复.
목적 관찰골수농축액-PGA지가복합재토관절연골수복중대골수자격적작용.방법 30지성년웅성신서란백토,마취후구건토적슬관절연골결손모형,장소유토자분위5개치료조:무치료조(공백대조)、골수자격(BMS)조、BMS+ PGA지가식입조、BMS+ PGA지가여자체골수농축액(BMC)복합물식입조화BMS+PGA지가여골수간세포(BME)복합물식입조.통과연골대체관、HE염색、갑분알람염색급면역조화등방법처리,평고골수농축기술대골수자격증강작용급토연골결손수복정황.결과 BMS후연골손상구평균MSC수량여종가척획득후MSC적수량대치상동,(26.3±6.0) MSCs비(27.6±6.5)MSCs.토연골결손구가견명현적신생연골형성,백색광활적수복조직전충결손구,여주위조직정합교호.HE염색견손상구이비상유서적조직전충사기표면규칙,병차여원연골조직정합교호；갑분알람염색、Ⅱ형효원단백면역조화염색교전3조경강、경평활、경규칙,ICRS시각조직학평분야현저고우전3조,여BME조결과상사.수복효과명현우우전3조.결론 골수농축액-PGA지가복합대골수자격구유증강작용,시일충간역、유효적방법,유망응용우관절연골적수복.
Objective To observe the effects of bone marrow concentrate (BMC)-PGA scaffolds for bone marrow stimulation enhancement and repairing rabbit articular cartilage.Methods A rabbit model of articular cartilage defect was established for BMC-PGA stent implantation.After 8 weeks,the experimental animals were sacrificed.And the methods of hematoxylin and eosin stain,toluidine blue stain and immunohistochemistry were used to evaluate the effects of bone marrow stimulation enhancement and rabbit cartilage defect repairing.Results Visible new cartilage formation was evident after implantation.As compared with other groups,the repairing effect was better.Conclusion The implantation of BMC-PGA scaffolds is both simple and effective in the repair of articular cartilage.