中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
39期
3152-3154
,共3页
胡远程%万圣云%秦丹丹%詹焱青%丁洋
鬍遠程%萬聖雲%秦丹丹%詹焱青%丁洋
호원정%만골운%진단단%첨염청%정양
投药,局部%止血药%颈动脉
投藥,跼部%止血藥%頸動脈
투약,국부%지혈약%경동맥
Administration,topical%Hemostatics%Carotid Arteries
目的 观察局部应用不同浓度的巴曲亭在新西兰兔颈动脉外膜剥离渗血模型中的止血效果.方法 取18只家兔,采用酶消化+钝性剥离的方法建立颈总动脉外膜剥离渗血模型,在此基础上局部用干纱布、盐水纱布、0.5、1、2及4 U/ml的巴曲亭纱布止血,HE、Masson染色及透射电镜观察造模方式是否成熟,并对比止血时间及出血量等指标来评价其止血效果.结果 酶消化+钝性剥离颈动脉外膜造成出血模型稳定性、重复性较好；0.5 U/ml巴曲亭的出血时间及单位面积出血量[(97 ±20)s,(0.102 ±0.013) g/cm2]较阴性对照[(143±33)s,(0.130 ±0.023) g/cm2]差异有统计学意义(P＜0.05)；而2 U/ml巴曲亭(32 ±13,0.056 ±0.015)较1 U/ml巴曲亭(65±26,0.080±0.014)差异仍有统计学意义(P＜0.05),但较4 U/ml巴曲亭(28±14,0.053±0.012)差异无统计学意义(P＞0.05),故巴曲亭的最佳局部止血浓度为2 U/ml.结论 局部应用巴曲亭对小面积渗血的止血效果良好.
目的 觀察跼部應用不同濃度的巴麯亭在新西蘭兔頸動脈外膜剝離滲血模型中的止血效果.方法 取18隻傢兔,採用酶消化+鈍性剝離的方法建立頸總動脈外膜剝離滲血模型,在此基礎上跼部用榦紗佈、鹽水紗佈、0.5、1、2及4 U/ml的巴麯亭紗佈止血,HE、Masson染色及透射電鏡觀察造模方式是否成熟,併對比止血時間及齣血量等指標來評價其止血效果.結果 酶消化+鈍性剝離頸動脈外膜造成齣血模型穩定性、重複性較好；0.5 U/ml巴麯亭的齣血時間及單位麵積齣血量[(97 ±20)s,(0.102 ±0.013) g/cm2]較陰性對照[(143±33)s,(0.130 ±0.023) g/cm2]差異有統計學意義(P＜0.05)；而2 U/ml巴麯亭(32 ±13,0.056 ±0.015)較1 U/ml巴麯亭(65±26,0.080±0.014)差異仍有統計學意義(P＜0.05),但較4 U/ml巴麯亭(28±14,0.053±0.012)差異無統計學意義(P＞0.05),故巴麯亭的最佳跼部止血濃度為2 U/ml.結論 跼部應用巴麯亭對小麵積滲血的止血效果良好.
목적 관찰국부응용불동농도적파곡정재신서란토경동맥외막박리삼혈모형중적지혈효과.방법 취18지가토,채용매소화+둔성박리적방법건립경총동맥외막박리삼혈모형,재차기출상국부용간사포、염수사포、0.5、1、2급4 U/ml적파곡정사포지혈,HE、Masson염색급투사전경관찰조모방식시부성숙,병대비지혈시간급출혈량등지표래평개기지혈효과.결과 매소화+둔성박리경동맥외막조성출혈모형은정성、중복성교호；0.5 U/ml파곡정적출혈시간급단위면적출혈량[(97 ±20)s,(0.102 ±0.013) g/cm2]교음성대조[(143±33)s,(0.130 ±0.023) g/cm2]차이유통계학의의(P＜0.05)；이2 U/ml파곡정(32 ±13,0.056 ±0.015)교1 U/ml파곡정(65±26,0.080±0.014)차이잉유통계학의의(P＜0.05),단교4 U/ml파곡정(28±14,0.053±0.012)차이무통계학의의(P＞0.05),고파곡정적최가국부지혈농도위2 U/ml.결론 국부응용파곡정대소면적삼혈적지혈효과량호.
Objective To observe the topically hemostatic effects of batroxobin (BX) in different concentrations on the carotid arteries adventitia removal rabbit.Methods 18 rabbits were removed vascular adventitia by collagenase digestion and mechanical dissection,causing capillary hemorrhage.Then all of them were randomly divided into 6 groups:blank control,negative control group,0.5,1,2 and 4 kU/L (U/ml) BX group.The hemostatic time and bleeding volume were observed to compare the hemostatic effect of each group.Haematoxylin-eosin,Masson staining and immunohistochemistry were performed to assure adventitia removed.Results It was feasible to remove vascular adventitia with collagenase digestion and mechanical dissection.The hemostatic time and bleeding volume were significantly different (P ＜ 0.05) from 0.5 U/ml BX group [(97 ± 20) s,(0.102 ± 0.013) g/cm2] of the negative control group [(143 ± 33) s,(0.130 _ 0.023) g/cm2].With the increase of BX concentration,there was a significant difference (P ＜ 0.05) between 2 U/ml BX group (32 ± 13,0.056 ± 0.015) and 1 U/ml BX group (32 ± 13,0.056 ± 0.015),but there was no statistical significance (P ＞ 0.05) between 2 U/ml BX group and 4 U/ml BX group (28 ± 14,0.053 ± 0.012).Thus,the best topical hemostatic concentration of BX was 2 U/ml.Conclusion The topical hemostatic effect of batroxobin is reliable in small area of blood oozing.
