CAJ | 학술논문

目的检测BALB/c小鼠哮喘模型的焦虑及抑郁状态.方法 20只小鼠按随机数字表法随机分为对照组和哮喘组各10只;哮喘组用鸡卵白蛋白加氢氧化铝致敏和激发,建立BALB/c小鼠哮喘模型,对照组用等量磷酸盐缓冲液代替.采用无创法检测不同浓度乙酰甲胆碱激发下2组小鼠的增强呼吸间歇(Penh)值来反映气道反应性.用高架十字迷宫法和强迫游泳法评估2组小鼠合并焦虑、抑郁程度.回收支气管肺泡灌洗液(BALF)进行细胞学分类.结果哮喘组和对照组小鼠Penh值均随乙酰甲胆碱浓度增加而增加,哮喘组小鼠在生理盐水和乙酰甲胆碱浓度为0、5 g/L激发后Penh值分别为0.43±0.04、0.41 ±0.05和0.44_±0.04,与对照组(0.42±0.03、0.39 ±0.03、0.43±0.04)差异均无统计学意义(P =0.290、0.652、0.723).在乙酰甲胆碱浓度为10、15、20 g/L时,哮喘组小鼠Penh值分别为0.57±0.03、0.85 ±0.04、1.57±0.08,均显著高于对照组的0.45±0.08、0.57 ±0.06、0.82±0.09(P =0.001、0.000、0.000).哮喘组小鼠总进臂次数[开臂进入次数(OE)+闭臂进入次数(CE)]、OE占OE+ CE百分比(OE％)、开臂滞留时问(OT)占总滞留时间[开臂滞留时间(OT)+闭臂滞留时间(CT)]百分比(OT％)分别为(18.3±3.6)次、(22.2±3.1)％、(16.7±4.2)％,显著低于与对照组的(24.0±2.9)次、(28.0±3.4)％、(21.8±4.6)％(P=0.001、0.001、0.019);游泳不动时间为(147±12)s,显著高于对照组的(133±10)s (P =0.010);BALF中细胞总数及嗜酸粒细胞比值[(10.0±4.0)×105/ml和(68.18±3.76)％]均显著高于对照组[(1.7±0.4)×105/ml和(0.12±0.07)％](均P=0.000).结论 BALB/c哮喘小鼠合并焦虑、抑郁状态,可应用该模型研究哮喘与焦虑、抑郁共病现象的分子生物学机制.
목적 검측BALB/c소서효천모형적초필급억욱상태.방법 20지소서안수궤수자표법수궤분위대조조화효천조각10지;효천조용계란백단백가경양화려치민화격발,건립BALB/c소서효천모형,대조조용등량린산염완충액대체.채용무창법검측불동농도을선갑담감격발하2조소서적증강호흡간헐(Penh)치래반영기도반응성.용고가십자미궁법화강박유영법평고2조소서합병초필、억욱정도.회수지기관폐포관세액(BALF)진행세포학분류.결과 효천조화대조조소서Penh치균수을선갑담감농도증가이증가,효천조소서재생리염수화을선갑담감농도위0、5 g/L격발후Penh치분별위0.43±0.04、0.41 ±0.05화0.44_±0.04,여대조조(0.42±0.03、0.39 ±0.03、0.43±0.04)차이균무통계학의의(P =0.290、0.652、0.723).재을선갑담감농도위10、15、20 g/L시,효천조소서Penh치분별위0.57±0.03、0.85 ±0.04、1.57±0.08,균현저고우대조조적0.45±0.08、0.57 ±0.06、0.82±0.09(P =0.001、0.000、0.000).효천조소서총진비차수[개비진입차수(OE)+폐비진입차수(CE)]、OE점OE+ CE백분비(OE％)、개비체류시문(OT)점총체류시간[개비체류시간(OT)+폐비체류시간(CT)]백분비(OT％)분별위(18.3±3.6)차、(22.2±3.1)％、(16.7±4.2)％,현저저우여대조조적(24.0±2.9)차、(28.0±3.4)％、(21.8±4.6)％(P=0.001、0.001、0.019);유영불동시간위(147±12)s,현저고우대조조적(133±10)s (P =0.010);BALF중세포총수급기산립세포비치[(10.0±4.0)×105/ml화(68.18±3.76)％]균현저고우대조조[(1.7±0.4)×105/ml화(0.12±0.07)％](균P=0.000).결론 BALB/c효천소서합병초필、억욱상태,가응용해모형연구효천여초필、억욱공병현상적분자생물학궤제.
Objective To detect the anxiety and depression status of BALB/c asthmatic model in mice.Methods A total of 20 mice were randomly divided into asthma and control groups (n =10 each).The animals were sensitized and challenged with ovalbumin for asthmatic model.Phosphate buffer solution was used in the control group.The mice were challenged with increasing concentrations of methacholine aerosol and the airway resistance was measuring non-invasively by enhanced pause (Penh).And elevated plus maze test system and forced swim were employed to evaluate their anxiety and depression status.Then bronchoalveolar lavage cytology was detected for evaluating the airway inflammation.Results In both groups,Penh value increased with the rising concentration of methacholine.The Penh values of asthmatic mice exciting with normal saline and methacholine concentration of 0,5 g/L were 0.43 _± 0.04,0.41 _± 0.05 and 0.44 ± 0.04 versus 0.42 ± 0.03,0.39 ± 0.03,0.43 _± 0.04 in control group.The difference had no statistical significance (P =0.290,0.652,0.723).At the concentration of methacholine 10,15,20 g/L,the Penh values of asthmatic mice were 0.57 ± 0.03,0.85 ± 0.04,1.57 ± 0.08 and they were significantly higher than those of control group (0.45 ± 0.08,0.57 ± 0.06,0.82 ± 0.09) (P =0.001,0.000,0.000).Asthmatic mice in the elevated plus maze on a behavioral test and found that asthmatic mice open arm entries sum closed arm entries (OE + CE),percent of open arm/total entries (OE％) and percent of open arm/total time (OT％) was (18.3 ± 3.6) times,(22.2 ± 3.1) ％,(16.7 ±_ 4.2) ％.They were significantly lower than those of control group ((24.0 ± 2.9) times,(28.0 ± 3.4)％,(21.8 ± 4.6)％) (P =0.001,0.001,0.019).Asthmatic mice swimming time was significantly higher than that of control group (147 ± 12) vs (133 ±_ 10) s (P =0.010).The total number of cells and eosinophil ratio in asthma group was (10.0 ±4.0) × 105/ml and (68.18 _± 3.76)％.And they were significantly larger than those of control group ((1.7 __ 0.4) × 105/ml,(0.12 ± 0.07) ％) (both P =0.000).Conclusion Asthmatic mice with concurrent anxiety and depression may be a model for elucidating the molecular mechanisms of asthma with anxiety and depression.