CAJ | 학술논문

目的观察顺铂联合高温对肿瘤手术回收血液中红细胞功能的影响及对其中HepG2、SGC7901与SW620细胞株的杀灭作用.方法回收2013年3-6月浙江大学医学院第二附属医院30例行择期手术的胃肠道肿瘤患者术中血,经医院伦理委员会通过并患者知情同意,制备成红细胞悬液,每1例分成3等份,分别加入HepG2、SGC7901与SW620细胞株,充分混匀后,每份细胞随机分为9组(n=30):A组(37 ℃),B组(42 ℃),C、D、E组(37 ℃,50、100、200 μg/ml顺铂),F、G、H、I组(42 ℃,25、50、100、200 μg/ml顺铂).所有组别均处理60 min后,分离红细胞和肿瘤细胞,测定红细胞Na+-K+-ATPase活力、红细胞计数、红细胞渗透脆性及细胞悬液血气指标,测定肿瘤细胞抑制率和培养14 d后克隆形成率.结果与A组[(0.30±0.08) μmol Pi/107/h]比较,E、H和I组的Na+-K+-ATPase活力[(0.24±0.07)、(0.25±0.06)和(0.24±0.07) μmol Pi/107/h]明显降低(P<0.05).与A组[(1.53±0.43) mmol/L]比较,E、H和I组K+[(2.16±0.37)、(2.16±0.38)和(2.56±0.50) mmol/L]明显升高(P<0.05).E、H和I组的渗透脆性与A组比较明显增加(P<0.05).B、C、D、E、F、G4组中,只有G组的HepG2、SGC7901及SW620的细胞克隆(0%±0%、0%±0%和0.01%±0.01%)被完全抑制,与A组比较差异有统计学意义(P<0.05,78.54%±7.83%、72.28%±6.58%和66.69%±6.69%).结论顺铂体外热化疗(42 ℃,50 μg/ml)处理回收血液60 min,可能是清除肿瘤术回收血液中游离肿瘤细胞有效手段,值得进一步优化并应用于肿瘤手术自体血液回输.
목적 관찰순박연합고온대종류수술회수혈액중홍세포공능적영향급대기중HepG2、SGC7901여SW620세포주적살멸작용.방법 회수2013년3-6월절강대학의학원제이부속의원30례행택기수술적위장도종류환자술중혈,경의원윤리위원회통과병환자지정동의,제비성홍세포현액,매1례분성3등빈,분별가입HepG2、SGC7901여SW620세포주,충분혼균후,매빈세포수궤분위9조(n=30):A조(37 ℃),B조(42 ℃),C、D、E조(37 ℃,50、100、200 μg/ml순박),F、G、H、I조(42 ℃,25、50、100、200 μg/ml순박).소유조별균처리60 min후,분리홍세포화종류세포,측정홍세포Na+-K+-ATPase활력、홍세포계수、홍세포삼투취성급세포현액혈기지표,측정종류세포억제솔화배양14 d후극륭형성솔.결과여A조[(0.30±0.08) μmol Pi/107/h]비교,E、H화I조적Na+-K+-ATPase활력[(0.24±0.07)、(0.25±0.06)화(0.24±0.07) μmol Pi/107/h]명현강저(P<0.05).여A조[(1.53±0.43) mmol/L]비교,E、H화I조K+[(2.16±0.37)、(2.16±0.38)화(2.56±0.50) mmol/L]명현승고(P<0.05).E、H화I조적삼투취성여A조비교명현증가(P<0.05).B、C、D、E、F、G4조중,지유G조적HepG2、SGC7901급SW620적세포극륭(0%±0%、0%±0%화0.01%±0.01%)피완전억제,여A조비교차이유통계학의의(P<0.05,78.54%±7.83%、72.28%±6.58%화66.69%±6.69%).결론 순박체외열화료(42 ℃,50 μg/ml)처리회수혈액60 min,가능시청제종류술회수혈액중유리종류세포유효수단,치득진일보우화병응용우종류수술자체혈액회수.
Objective To investigate the effects of cisplatin plus hyperthermia on erythrocytes and killing human hepatocarcinoma (HepG2), gastrocarcinoma (SGC7901) and colonic carcinoma (SW620) cells in the intra-operative blood salvage from cancer surgery in vitro. Methods HepG2, SGC7901 or SW620 cells were mixed into the aliquot of erythrocyte concentrated from each intra-operative blood salvage of 30 patients subjected to gastrointestinal cancer surgery. The mixture cells were divided into the following groups (n=30): A group (37 ℃); B group (42 ℃); C, D, E groups (50, 100, or 200 μg/ml DDP); F, G, H, I groups (42 ℃, 25, 50, 100, or 200 μg/ml DDP). After treating for 60 min, tumor cells and erythrocytes were separated by density gradient centrifugation. The Na+-K+-ATPase activity, cell count, osmotic fragility, and blood gas variables were determined in erythrocytes. Cell viability and colony formation were determined in tumor cells. Results Compared with A [(0.30±0.08) μmol Pi/107/h], the Na+-K+-ATPase activity was significantly decreased in E, H and I groups [(0.24±0.07), (0.25±0.06) and (0.24±0.07) μmol Pi/107/h] (P<0.05). Extra-erythrocytic K+ in E, H and I groups [(2.16±0.37), (2.16±0.38) and (2.56±0.50) mmol/L] were significantly increased compared with A group [(1.53±0.43) mmol/L] (P<0.05). Compared with A group, osmotic fragility in E, H and I groups was significantly increased (P<0.05). Among B, C, D, E, F, G groups, only in G group colony formations of HepG2, SGC7901, and SW620 (0%±0%, 0%±0% and 0.01%±0.01%) at 14 d were completely inhibited (P<0.01) compared with A group (78.54%±7.83%, 72.28%±6.58% and 66.69%±6.69%). Conclusion Pretreatment with cisplatin (50 μg/ml) plus hyperthermia (42 ℃) for 60 min in vitro might be an effective strategy to clear tumor cells contamination but preserve erythrocytes, which is worthy to be optimized and used in the intra-operative blood salvage in cancer surgery.