中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
10期
776-779
,共4页
陈晖%叶力%苏锦明%蒋敦科%李彧%曾锦荣%阮族明
陳暉%葉力%囌錦明%蔣敦科%李彧%曾錦榮%阮族明
진휘%협력%소금명%장돈과%리욱%증금영%원족명
干扰素类%肝炎,丙型%微RNAs
榦擾素類%肝炎,丙型%微RNAs
간우소류%간염,병형%미RNAs
Interferons%Hepatitis C%MicroRNAs
目的 探讨不同类型干扰素(IFN)对肝细胞丙型肝炎病毒(HCV)复制相关微RNA(miRNA)表达的影响.方法 用不同浓度的Ⅰ型(IFN-α、IFN-β)、Ⅱ型(IFN-γ)和Ⅲ型(IFN-λ1)IFN处理Huh7细胞24 h,用未经处理的Huh7细胞作为对照,采用实时荧光定量PCR检测Huh7细胞中HCV复制相关miRNA的表达及其差异,实验均重复4次.结果 IFN-α、IFN-β、IFN-λ1组miRNA-196、miRNA-296、miRNA-351、miRNA-431和miRNA-448的表达均高于对照组(474.5±57.7、436.0±37.8、431.3±43.5比99.5 ±2.4,389.5 ±44.0、416.3 ±36.7、284.8±20.9比99.0±0.8,453.3±51.1、473.8±24.0、522.0±40.3比99.5 ±2.1,288.3 ±44.9、294.3±29.9、450.0±29.5比100.5±2.4,319.0 ±35.4、357.0±27.5、446.5 ±61.2比99.8±1.0,均P＜0.05).IFN-γ组miRNA-196、miRNA-351和miRNA-448的表达(271.3±29.6、140.5 ±27.5、177.8±23.4)均高于对照组(均P＜0.05),而miRNA-296和miRNA-431的表达与对照组差异均无统计学意义(均P＞0.05).不同浓度(10、100、1 000 U/ml)的IFN-α、IFN-β、IFN-γ和10、100、1 000 ng/ml IFN-λ1处理组的miRNA-122表达均低于对照组(49.3 ±4.9、32.0±3.7、24.5±3.7比99.5 ±2.1,53.5±1.9、35.5 ±2.7、25.8±4.0比98.8±1.3,60.8±7.1、43.8 ±4.0、33.5±4.2比99.3 ±2.6和50.0±2.9、35.5±3.3、22.3 ±3.8比100.3 ±1.5,均P＜0.01).结论 不同类型IFN均具有上调抑制HCV复制miRNA的能力,同时可以下调支持HCV复制的miRNA-122的表达,表明调节HCV复制相关miRNA表达是不同类型IFN抗HCV的一个普遍机制.而Ⅱ型IFN与其他类型IFN调节HCV复制相关miRNA表达的模式有所不同,表明Ⅱ型IFN与Ⅰ、Ⅲ型IFN在miRNA水平上具有不同的抗病毒机制.
目的 探討不同類型榦擾素(IFN)對肝細胞丙型肝炎病毒(HCV)複製相關微RNA(miRNA)錶達的影響.方法 用不同濃度的Ⅰ型(IFN-α、IFN-β)、Ⅱ型(IFN-γ)和Ⅲ型(IFN-λ1)IFN處理Huh7細胞24 h,用未經處理的Huh7細胞作為對照,採用實時熒光定量PCR檢測Huh7細胞中HCV複製相關miRNA的錶達及其差異,實驗均重複4次.結果 IFN-α、IFN-β、IFN-λ1組miRNA-196、miRNA-296、miRNA-351、miRNA-431和miRNA-448的錶達均高于對照組(474.5±57.7、436.0±37.8、431.3±43.5比99.5 ±2.4,389.5 ±44.0、416.3 ±36.7、284.8±20.9比99.0±0.8,453.3±51.1、473.8±24.0、522.0±40.3比99.5 ±2.1,288.3 ±44.9、294.3±29.9、450.0±29.5比100.5±2.4,319.0 ±35.4、357.0±27.5、446.5 ±61.2比99.8±1.0,均P＜0.05).IFN-γ組miRNA-196、miRNA-351和miRNA-448的錶達(271.3±29.6、140.5 ±27.5、177.8±23.4)均高于對照組(均P＜0.05),而miRNA-296和miRNA-431的錶達與對照組差異均無統計學意義(均P＞0.05).不同濃度(10、100、1 000 U/ml)的IFN-α、IFN-β、IFN-γ和10、100、1 000 ng/ml IFN-λ1處理組的miRNA-122錶達均低于對照組(49.3 ±4.9、32.0±3.7、24.5±3.7比99.5 ±2.1,53.5±1.9、35.5 ±2.7、25.8±4.0比98.8±1.3,60.8±7.1、43.8 ±4.0、33.5±4.2比99.3 ±2.6和50.0±2.9、35.5±3.3、22.3 ±3.8比100.3 ±1.5,均P＜0.01).結論 不同類型IFN均具有上調抑製HCV複製miRNA的能力,同時可以下調支持HCV複製的miRNA-122的錶達,錶明調節HCV複製相關miRNA錶達是不同類型IFN抗HCV的一箇普遍機製.而Ⅱ型IFN與其他類型IFN調節HCV複製相關miRNA錶達的模式有所不同,錶明Ⅱ型IFN與Ⅰ、Ⅲ型IFN在miRNA水平上具有不同的抗病毒機製.
목적 탐토불동류형간우소(IFN)대간세포병형간염병독(HCV)복제상관미RNA(miRNA)표체적영향.방법 용불동농도적Ⅰ형(IFN-α、IFN-β)、Ⅱ형(IFN-γ)화Ⅲ형(IFN-λ1)IFN처리Huh7세포24 h,용미경처리적Huh7세포작위대조,채용실시형광정량PCR검측Huh7세포중HCV복제상관miRNA적표체급기차이,실험균중복4차.결과 IFN-α、IFN-β、IFN-λ1조miRNA-196、miRNA-296、miRNA-351、miRNA-431화miRNA-448적표체균고우대조조(474.5±57.7、436.0±37.8、431.3±43.5비99.5 ±2.4,389.5 ±44.0、416.3 ±36.7、284.8±20.9비99.0±0.8,453.3±51.1、473.8±24.0、522.0±40.3비99.5 ±2.1,288.3 ±44.9、294.3±29.9、450.0±29.5비100.5±2.4,319.0 ±35.4、357.0±27.5、446.5 ±61.2비99.8±1.0,균P＜0.05).IFN-γ조miRNA-196、miRNA-351화miRNA-448적표체(271.3±29.6、140.5 ±27.5、177.8±23.4)균고우대조조(균P＜0.05),이miRNA-296화miRNA-431적표체여대조조차이균무통계학의의(균P＞0.05).불동농도(10、100、1 000 U/ml)적IFN-α、IFN-β、IFN-γ화10、100、1 000 ng/ml IFN-λ1처리조적miRNA-122표체균저우대조조(49.3 ±4.9、32.0±3.7、24.5±3.7비99.5 ±2.1,53.5±1.9、35.5 ±2.7、25.8±4.0비98.8±1.3,60.8±7.1、43.8 ±4.0、33.5±4.2비99.3 ±2.6화50.0±2.9、35.5±3.3、22.3 ±3.8비100.3 ±1.5,균P＜0.01).결론 불동류형IFN균구유상조억제HCV복제miRNA적능력,동시가이하조지지HCV복제적miRNA-122적표체,표명조절HCV복제상관miRNA표체시불동류형IFN항HCV적일개보편궤제.이Ⅱ형IFN여기타류형IFN조절HCV복제상관miRNA표체적모식유소불동,표명Ⅱ형IFN여Ⅰ、Ⅲ형IFN재miRNA수평상구유불동적항병독궤제.
Objective To explore the effects of different types of interferon (IFN) on the expression of hepatitis C virus (HCV) replication-related miRNAs in human hepatocytes.Methods The levels of miRNAs in Huh7 cells treated with types Ⅰ (IFN-α,IFN-β),Ⅱ (IFN-γ) and Ⅲ (IFN-λ1) interferons (IFNs) were determined by real-time quantitative polymerase chain reaction (PCR).The levels of various miRNAs were compared among different IFN treatment groups.Results The expression of miRNA-196,miRNA-296,miRNA-351,miRNA-431 and miRNA-448 in IFN-α,IFN-β and IFN-λ1 treated groups was higher than that in control group (474.5 ± 57.7,436.0 ± 37.8,431.3 ± 43.5 vs 99.5 ± 2.4 ; 389.5 ± 44.0,416.3 ± 36.7,284.8 ± 20.9 vs 99.0 ± 0.8; 453.3 ± 51.1,473.8 ± 24.0,522.0 ± 40.3 vs 99.5 ±2.1; 288.3 ±44.9,294.3 ± 29.9,450.0 ± 29.5 vs 100.5 ±2.4; 319.0 ±35.4,357.0 ±27.5,446.5 ± 61.2 vs 99.8 ± 1.0 ; all P ＜ 0.05).The expression of miRNA-196,miRNA-351 and miRNA-448 in IFN-γ-treated group (271.3 ±29.6,140.5 ±27.5,177.8 ±23.4) was higher than that in control group (all P ＜0.05).But the expression of miRNA-431 and miRNA-296 had no significant difference between IFN-γ treated group and control group (both P ＞ 0.05).The expression of miRNA-122 at difference concentrations (10,100,1 000 U/ml) of IFN-α,IFN-β,IFN-γand IFN-λ1 (10,100,1 000 ng/ml) was lower than that in control gourp (49.3 ± 4.9,32.0 ± 3.7,24.5 ± 3.7 vs 99.5 ± 2.1 ; 53.5 ± 1.9,35.5 ± 2.7,25.8±4.0vs98.8±1.3;60.8±7.1,43.8±4.0,33.5±4.2vs99.3±2.6; 50.0±2.9,35.5± 3.3,22.3 ± 3.8 vs 100.3 ± 1.5 ; all P ＜ 0.01).Conclusions Different types of IFN increase the expression of miRNAs that inhibit HCV replication and decrease the expression of miRNA-122 that supports HCV replication.Thus the regulation of HCV replication-related miRNA expression is a general anti-HCV mechanism by different types of IFN.However,the regulation pattem by type]Ⅱ IFN is different from those by other types of IFNs,implying that type Ⅱ IFN has different anti-HCV mechanisms from other types of IFN at miRNA level.
