中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
12期
928-931
,共4页
王林娜%樊硕%郑和义%李军%姚志远
王林娜%樊碩%鄭和義%李軍%姚誌遠
왕림나%번석%정화의%리군%요지원
梅毒%密螺旋体,苍白%分子分型
梅毒%密螺鏇體,蒼白%分子分型
매독%밀라선체,창백%분자분형
Syphilis%Treponema pallidum%Molecular typing
目的 探讨外周血标本检测梅毒螺旋体DNA基因分型的可行性,了解梅毒病程与梅毒螺旋体DNA阳性率及基因分型的关系.方法 收集2012年7月至2013年2月北京3家医院性病中心就诊的181例梅毒患者的临床资料,采用PCR方法检测患者外周血梅毒螺旋体特异性基因polA基因;对pol A基因阳性标本采用巢式PCR法扩增酸性重复蛋白质基因arp和重复基因簇基因tpr进行基因分型.结果 181例梅毒患者中有35例polA基因阳性,其中67例初诊非潜伏梅毒的未治疗患者中24例(35.8％)阳性;26例潜伏梅毒患者均阴性;72例复诊患者中7例(9.7％)阳性;16例血清固定患者中4例(25.0％)阳性.进一步基因分型发现35份polA基因阳性标本中14份为14d亚型,10份为14b亚型,7份为14a亚型,4份为13c亚型,其中血清固定的4份标本中3份为14d亚型,1份为14b亚型.结论 外周血检测梅毒螺旋体基因具有可行性,尽管阳性率较低,但是可靠易行,尤其对血清固定患者具有一定意义.
目的 探討外週血標本檢測梅毒螺鏇體DNA基因分型的可行性,瞭解梅毒病程與梅毒螺鏇體DNA暘性率及基因分型的關繫.方法 收集2012年7月至2013年2月北京3傢醫院性病中心就診的181例梅毒患者的臨床資料,採用PCR方法檢測患者外週血梅毒螺鏇體特異性基因polA基因;對pol A基因暘性標本採用巢式PCR法擴增痠性重複蛋白質基因arp和重複基因簇基因tpr進行基因分型.結果 181例梅毒患者中有35例polA基因暘性,其中67例初診非潛伏梅毒的未治療患者中24例(35.8％)暘性;26例潛伏梅毒患者均陰性;72例複診患者中7例(9.7％)暘性;16例血清固定患者中4例(25.0％)暘性.進一步基因分型髮現35份polA基因暘性標本中14份為14d亞型,10份為14b亞型,7份為14a亞型,4份為13c亞型,其中血清固定的4份標本中3份為14d亞型,1份為14b亞型.結論 外週血檢測梅毒螺鏇體基因具有可行性,儘管暘性率較低,但是可靠易行,尤其對血清固定患者具有一定意義.
목적 탐토외주혈표본검측매독라선체DNA기인분형적가행성,료해매독병정여매독라선체DNA양성솔급기인분형적관계.방법 수집2012년7월지2013년2월북경3가의원성병중심취진적181례매독환자적림상자료,채용PCR방법검측환자외주혈매독라선체특이성기인polA기인;대pol A기인양성표본채용소식PCR법확증산성중복단백질기인arp화중복기인족기인tpr진행기인분형.결과 181례매독환자중유35례polA기인양성,기중67례초진비잠복매독적미치료환자중24례(35.8％)양성;26례잠복매독환자균음성;72례복진환자중7례(9.7％)양성;16례혈청고정환자중4례(25.0％)양성.진일보기인분형발현35빈polA기인양성표본중14빈위14d아형,10빈위14b아형,7빈위14a아형,4빈위13c아형,기중혈청고정적4빈표본중3빈위14d아형,1빈위14b아형.결론 외주혈검측매독라선체기인구유가행성,진관양성솔교저,단시가고역행,우기대혈청고정환자구유일정의의.
Objective To detect Treponema pallidum (T.pallidum) DNA with polymerase chain reaction (PCR) in whole blood samples of syphilis patients and analyze their features of subgenotypes.Methods The clinical data of patients were collected from July 2012 to February 2013.And polA gene of syphilis was detected by PCR.The arp and tpr genes of polA gene-positive samples were analyzed by the established genotyping system.Statistical analyses were performed to compare different clinical courses and features to examine their correlations.Results The common treponemal gene target (polA) of 35 samples were detected in whole blood by PCR in 181 samples.A total of 24 cases (35.8％) were positive in 67 patients with newly diagnosed non-latent syphilis untreated patients;26 cases of latent syphilis were negative;7 cases (9.7％) were positive in 72 subsequent visit patients;4 cases (25.0％) were positive in 16 patients with sero-resistant.There were 4 subtypes of 14a (n =7),14b (n =10),13c (n =4) and 14d (n =14).Among those positive samples,there were 4 sero-resistant samples of 3 subtypes 14d and 1 subtype 14b.Conclusions The feasibility of peripheral blood is confirmed.Although the positive rate of whole blood detection of T.pallidum gene is low,the method is both simple and reliable for patients with sero-resistant syphilis.
