中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
18期
1416-1418
,共3页
庞霞%马跃伟%曲蕴慧%杨海军%高冬玲
龐霞%馬躍偉%麯蘊慧%楊海軍%高鼕玲
방하%마약위%곡온혜%양해군%고동령
食管%肿瘤,鳞状细胞%基因,肿瘤抑制%丝裂原活化蛋白激酶3
食管%腫瘤,鱗狀細胞%基因,腫瘤抑製%絲裂原活化蛋白激酶3
식관%종류,린상세포%기인,종류억제%사렬원활화단백격매3
Esophagus%Neoplasms,squamous cell%Genes,tumor suppressor%Mitogenactivated protein kinase 3
目的 探讨食管鳞状细胞癌(简称鳞癌)组织中抑癌基因Dab2和细胞外信号调节激酶1(ERK1)表达的临床意义.方法 采用免疫组化、原位杂交及反转录-PCR技术检测59例食管鳞癌组织、27例不典型增生组织和36例正常食管黏膜组织中Dab2和ERK1蛋白及mRNA的表达.结果 食管鳞癌组织中Dab2蛋白及mRNA表达阳性率显著低于其他两种组织(23.7％比94.4％、44.4％及18.6％比97.2％、33.3％),而ERK1蛋白及mRNA表达阳性率显著高于其他两种组织(81.4％比44.4％、63.0％及78.0％比30.6％、51.9％)(均P＜0.05).Dab2、ERK1蛋白及mRNA表达与淋巴结转移显著相关,且ERK1蛋白及mRNA表达与肿瘤浸润深度显著相关(均P＜0.05).食管鳞癌组织中Dab2与ERK1的表达呈负相关(P＜0.05).结论 Dab2和ERK1与食管鳞癌的发生、发展和转移密切相关,其作用可能互为拮抗.
目的 探討食管鱗狀細胞癌(簡稱鱗癌)組織中抑癌基因Dab2和細胞外信號調節激酶1(ERK1)錶達的臨床意義.方法 採用免疫組化、原位雜交及反轉錄-PCR技術檢測59例食管鱗癌組織、27例不典型增生組織和36例正常食管黏膜組織中Dab2和ERK1蛋白及mRNA的錶達.結果 食管鱗癌組織中Dab2蛋白及mRNA錶達暘性率顯著低于其他兩種組織(23.7％比94.4％、44.4％及18.6％比97.2％、33.3％),而ERK1蛋白及mRNA錶達暘性率顯著高于其他兩種組織(81.4％比44.4％、63.0％及78.0％比30.6％、51.9％)(均P＜0.05).Dab2、ERK1蛋白及mRNA錶達與淋巴結轉移顯著相關,且ERK1蛋白及mRNA錶達與腫瘤浸潤深度顯著相關(均P＜0.05).食管鱗癌組織中Dab2與ERK1的錶達呈負相關(P＜0.05).結論 Dab2和ERK1與食管鱗癌的髮生、髮展和轉移密切相關,其作用可能互為拮抗.
목적 탐토식관린상세포암(간칭린암)조직중억암기인Dab2화세포외신호조절격매1(ERK1)표체적림상의의.방법 채용면역조화、원위잡교급반전록-PCR기술검측59례식관린암조직、27례불전형증생조직화36례정상식관점막조직중Dab2화ERK1단백급mRNA적표체.결과 식관린암조직중Dab2단백급mRNA표체양성솔현저저우기타량충조직(23.7％비94.4％、44.4％급18.6％비97.2％、33.3％),이ERK1단백급mRNA표체양성솔현저고우기타량충조직(81.4％비44.4％、63.0％급78.0％비30.6％、51.9％)(균P＜0.05).Dab2、ERK1단백급mRNA표체여림파결전이현저상관,차ERK1단백급mRNA표체여종류침윤심도현저상관(균P＜0.05).식관린암조직중Dab2여ERK1적표체정부상관(P＜0.05).결론 Dab2화ERK1여식관린암적발생、발전화전이밀절상관,기작용가능호위길항.
Objective To explore the expressions of protein and mRNA of disabled-2 (Dab2) and extracellular signal-regulated kinase1 (ERK1) in esophageal squamous cell carcinoma tissue (ESCC) and their clinical significance.Methods The expressions of protein and mRNA of Dab2 and ERK1 were detected in ESCC (n =59),atypical hyperplasia (n =27) and normal esophageal mucosa (n =36) tissues by immunohistochemistry,in situ hybridization and reverse transcription polymerase chain reaction (RTPCR).Results The expressions of protein and mRNA of Dab2 were lesser in ESCC than those in atypical hyperplasia and normal esophageal mucosa tissues (23.7％ vs 94.4％,44.4％ and 18.6％ vs 97.2％,33.3％).And the expressions of protein and mRNA ERK1 were higher in ESCC than those in atypical hyperplasia and normal esophageal mucosa tissues (81.4％ vs 44.4％,63.0％ and 78.0％ vs 30.6％,51.9％) (all P ＜ 0.05).Additionally,the expressions of protein and mRNA of Dab2 and ERK1 were closely related with lymph node metastasis in ESCC tissue and the expressions of protein and mRNA of ERK1 closely related with the depth of tumor invasion in ESCC tissue (all P ＜ 0.05).Furthermore,the results of correlation analysis revealed that expressions of protein and mRNA of Dab2 and ERK1 in ESCC had a negative correlation (all P ＜ 0.05).Conclusion The expressions of protein and mRNA of Dab2 and ERK1 play important roles in the infiltration,metastasis and carcinogenesis of ESCC and their effects may be antagonistic to each other.
