中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
19期
1483-1487
,共5页
汪春娟%杨锦珊%林华伟%付佩彩%郭守刚%唐荣华
汪春娟%楊錦珊%林華偉%付珮綵%郭守剛%唐榮華
왕춘연%양금산%림화위%부패채%곽수강%당영화
嗅鞘细胞%神经干细胞%脑出血%神经营养因子-3
嗅鞘細胞%神經榦細胞%腦齣血%神經營養因子-3
후초세포%신경간세포%뇌출혈%신경영양인자-3
Olfactory ensheathing cells%Neural stem cell%Hemorrhage%Neurotrophin-3
目的 观察神经营养因子-3(NT-3)基因修饰嗅鞘细胞-神经干细胞(OECs-NSCs)复合细胞支架自体移植对脑出血大鼠运动功能的影响.方法 建立Wistar大鼠尾状核脑出血模型,完全随机化分为A组:NT-3基因修饰的OECs-NSCs-复合细胞支架移植组;B组:NT3-OECs-NSCs-复合细胞移植组;C组:支架移植组;D组:空白对照组.各组移植后第1、3、7、14和30天进行运动神经功能评分,并经免疫组织化学染色及荧光染色鉴定移植细胞在大鼠脑内的生存、分布及分化情况.结果 A、B组在移植后第7、14、30天神经功能评分分别为(2.12 ±0.12、1.50±0.11、0.52±0.08)、(2.10±0.16、1.79±0.09、0.91±0.10)明显低于C、D组;C组与D组间差异无统计学意义(P＞0.05).在第14天和第30天,A组评分明显低于B组(P ＜0.05,P＜0.01).免疫组织化学方法证实A组移植后存活的NSCs数量较多、向损伤区迁徙的细胞较多.结论 NT-3基因修饰OECs-NSCS-复合细胞支架移植治疗能显著改善脑出血大鼠的运动功能,且移植的NSCs具有较强的存活、迁移及分化能力.
目的 觀察神經營養因子-3(NT-3)基因脩飾嗅鞘細胞-神經榦細胞(OECs-NSCs)複閤細胞支架自體移植對腦齣血大鼠運動功能的影響.方法 建立Wistar大鼠尾狀覈腦齣血模型,完全隨機化分為A組:NT-3基因脩飾的OECs-NSCs-複閤細胞支架移植組;B組:NT3-OECs-NSCs-複閤細胞移植組;C組:支架移植組;D組:空白對照組.各組移植後第1、3、7、14和30天進行運動神經功能評分,併經免疫組織化學染色及熒光染色鑒定移植細胞在大鼠腦內的生存、分佈及分化情況.結果 A、B組在移植後第7、14、30天神經功能評分分彆為(2.12 ±0.12、1.50±0.11、0.52±0.08)、(2.10±0.16、1.79±0.09、0.91±0.10)明顯低于C、D組;C組與D組間差異無統計學意義(P＞0.05).在第14天和第30天,A組評分明顯低于B組(P ＜0.05,P＜0.01).免疫組織化學方法證實A組移植後存活的NSCs數量較多、嚮損傷區遷徙的細胞較多.結論 NT-3基因脩飾OECs-NSCS-複閤細胞支架移植治療能顯著改善腦齣血大鼠的運動功能,且移植的NSCs具有較彊的存活、遷移及分化能力.
목적 관찰신경영양인자-3(NT-3)기인수식후초세포-신경간세포(OECs-NSCs)복합세포지가자체이식대뇌출혈대서운동공능적영향.방법 건립Wistar대서미상핵뇌출혈모형,완전수궤화분위A조:NT-3기인수식적OECs-NSCs-복합세포지가이식조;B조:NT3-OECs-NSCs-복합세포이식조;C조:지가이식조;D조:공백대조조.각조이식후제1、3、7、14화30천진행운동신경공능평분,병경면역조직화학염색급형광염색감정이식세포재대서뇌내적생존、분포급분화정황.결과 A、B조재이식후제7、14、30천신경공능평분분별위(2.12 ±0.12、1.50±0.11、0.52±0.08)、(2.10±0.16、1.79±0.09、0.91±0.10)명현저우C、D조;C조여D조간차이무통계학의의(P＞0.05).재제14천화제30천,A조평분명현저우B조(P ＜0.05,P＜0.01).면역조직화학방법증실A조이식후존활적NSCs수량교다、향손상구천사적세포교다.결론 NT-3기인수식OECs-NSCS-복합세포지가이식치료능현저개선뇌출혈대서적운동공능,차이식적NSCs구유교강적존활、천이급분화능력.
Objective To explore the effects of autotransplantation of NT-3 gene modified olfactory ensheathing cell (OEC) and neural stem cell (NSC) complex adhering to collagen protein-heparin sulfate scaffold on motor function of rats with cerebral hemorrhage.Methods The cerebral hemorrhage model was established with caudate nucleus bleeding in Wistar rats.The animals were randomly divided into 4 groups:A (transplantation of NT-3 modified OEC-NSC complex adhering to scaffold),B (transplantation of NT-3 modified OEC-NSC complex),C (transplantation of scaffold) and D (blank control group).The motor function of hind limbs was assessed at Day 1,3,7,14 and 30 post-transplantation respectively.The survival,distribution and differentiation of transplanted cells were tested by immunohistochemistry and fluorescent staining.Results The neurological functional score of group A (2.12 ± 0.12,1.50 ± 0.11,0.52 ±0.08)or B(2.10 ±0.16,1.79 ±0.09,0.91 ±0.10)was obviously inferior to group C/D at Days 7,14 and 30.No significant difference existed between groups C and D (P ＞ 0.05).The scores of A were markedly lower than those of B at Days 14 and 30 (P ＜ 0.05,P ＜ 0.01).The numbers of surviving NSCs and cells migrating to focal area after transplantation was much more in group A than those in other groups.Conclusion Autotransplantation of NT-3 gene modified OEC-NSC complex adhering to collagen proteinheparin sulfate scaffold may markedly ameliorate the motor function of cerebral hemorrhagic in rats.And the transplanted NSCs have better capacities of survival,migration and differentiation.
