CAJ | 학술논문

目的探讨基于快速成型技术制作的多孔钛合金支架的体外细胞相容性及修复节段性骨缺损的疗效.方法利用选择性激光烧结技术制作的多孔可控微结构的钛合金支架,同时使用酸蚀法对其表面进行改性,分为未处理支架组和酸蚀处理组;将小鼠前成骨细胞与两组支架进行复合培养,定期检测材料对细胞黏附、增殖和分化的影响;选取新西兰大白兔15只,制作桡骨中段骨缺损模型并植入支架,定期采用X线观察,并于术后12周取离体标本进行Micro-CT的骨体积分数测量及组织学观察.结果支架两实验组支架上的细胞随着时间的延长(14 d),ALP活性进行性增加(0.83±0.09比0.82±0.08,P＞0.05);Micro-CT及组织学结果提示钛合金支架组孔内长入大量新生骨,与空白对照组相比差异有统计学意义[(25.4％±4.2％)比(23.6％ ±8.4％)比(12.3％±4.7％),P＜0.05],但两实验组间差异无统计学意义[(25.4％±4.2％)比(23.6％ ±8.4％),P＞0.05].结论 SLS制作的多孔钛合金支架展现了良好的体外相容性,能够有利于长段骨缺损的修复,不仅能够避免“应力遮挡”现象,且具有良好的骨传导性.
목적 탐토기우쾌속성형기술제작적다공태합금지가적체외세포상용성급수복절단성골결손적료효.방법 이용선택성격광소결기술제작적다공가공미결구적태합금지가,동시사용산식법대기표면진행개성,분위미처리지가조화산식처리조;장소서전성골세포여량조지가진행복합배양,정기검측재료대세포점부、증식화분화적영향;선취신서란대백토15지,제작뇨골중단골결손모형병식입지가,정기채용X선관찰,병우술후12주취리체표본진행Micro-CT적골체적분수측량급조직학관찰.결과 지가량실험조지가상적세포수착시간적연장(14 d),ALP활성진행성증가(0.83±0.09비0.82±0.08,P＞0.05);Micro-CT급조직학결과제시태합금지가조공내장입대량신생골,여공백대조조상비차이유통계학의의[(25.4％±4.2％)비(23.6％ ±8.4％)비(12.3％±4.7％),P＜0.05],단량실험조간차이무통계학의의[(25.4％±4.2％)비(23.6％ ±8.4％),P＞0.05].결론 SLS제작적다공태합금지가전현료량호적체외상용성,능구유리우장단골결손적수복,불부능구피면“응력차당”현상,차구유량호적골전도성.
Objective To evaluate the biocompatibility of SLS-produced titanium alloy scaffold in vitro and investigate the therapeutic effects in repairing segmental bone defects.Methods Porous titanium alloy scaffolds were produced by SLS and their surfaces were either left untreated or acid etched.In vitro,mouse pre-osteoblasts (MC3T3-E1 cells) were cultured on these 2 group scaffolds,and then cell proliferation and differentiation were examined after cell seeding.In vivo,bone defects were artificially made in 15 New Zealand rabbits and the porous titanium specimens were implanted into the radius of rabbits for 3 months.The regulating checks of X-ray were determined.The osteointegration of the implants was investigated by Micro-CT and histological examination at 12 weeks after surgery.Results A gradual increase in cell-specific ALP synthesis by cells cultured in both groups was observed with longer culture time (14 d).ALP activity did not differ significantly between two groups (0.834 ± 0.092 vs 0.815 ± 0.081,P ＞ 0.05).Both Micro-CT and the histological analysis indicated that the titanium alloy scaffolds had excellent ability to facilitate the osteointegration in vivo.The results were significantly different between the empty control and the 2 different surface modifications of SLS-implants (25.4％ ± 4.2％ vs 23.6％ ± 8.4％ vs 12.3％ ± 4.7％,P ＜0.05).Between the groups with implants,the number of bone points was not significantly different,irrespective of the surface treatment (25.4％ ±4.2％ vs 23.6％ ±8.4％,P＞0.05).Conclusion Selective Laser Sintering-Produced porous titanium alloy scaffold possessed admirable biocompatibility in vitro.It also could be contributed to the healing of long tulular bone defect.The porous Ti6Al4V implant not only reduced the stress-shielding but also exerted appropriate osteoconductive properties.