CAJ | 학술논문

目的探讨于大鼠脊髓急性压迫损伤急性期局部应用4-氨基吡啶-3-甲醇(4-AP-3-MeOH)后脊髓神经传导功能改善情况.方法 12只成年雄性SD大鼠随机分为两组:实验组6只、对照组6只,分别于脊髓T11节段压迫损伤后30 min局部应用1 ml 4-AP-3-MeOH(100 μmol/ml)或1ml生理盐水.测定两组压迫损伤前、压迫损伤后30 min、用药干预后体表感觉诱发电位(SSEP)波幅值,并处死动物行脊髓组织LFB染色观察.结果实验组及对照组压迫损伤前、压迫损伤后30 min及用药干预后SSEP值分别为(1.26 ±0.35)、(0.03 ±0.05)、(0.45 ±0.19) μv,(1.05 ±0.39)、(0.01±0.02)、(0.02±0.02) μv.用药干预后SSEP与压迫损伤后30 min SSEP相比,实验组差异有统计学意义(P＜0.01),对照组差异无统计学意义(P＞0.05).脊髓急性压迫损伤后30 min,脊髓大体照可见损伤部位肿胀,出血,劳克坚牢蓝(LFB)染色可见灰质、白质出血水肿,中央管破坏,白质结构破坏、不同程度脱髓鞘病变.结论脊髓急性压迫损伤后30 min即存在灰白质结构出血、白质脱髓鞘等病理变化,于脊髓损伤局部应用新型钾离子通道阻滞剂4-AP-3-MeOH可显著改善脊髓压迫损伤大鼠SSEP传导,促进损伤脊髓的神经传导功能恢复.
목적 탐토우대서척수급성압박손상급성기국부응용4-안기필정-3-갑순(4-AP-3-MeOH)후척수신경전도공능개선정황.방법 12지성년웅성SD대서수궤분위량조:실험조6지、대조조6지,분별우척수T11절단압박손상후30 min국부응용1 ml 4-AP-3-MeOH(100 μmol/ml)혹1ml생리염수.측정량조압박손상전、압박손상후30 min、용약간예후체표감각유발전위(SSEP)파폭치,병처사동물행척수조직LFB염색관찰.결과 실험조급대조조압박손상전、압박손상후30 min급용약간예후SSEP치분별위(1.26 ±0.35)、(0.03 ±0.05)、(0.45 ±0.19) μv,(1.05 ±0.39)、(0.01±0.02)、(0.02±0.02) μv.용약간예후SSEP여압박손상후30 min SSEP상비,실험조차이유통계학의의(P＜0.01),대조조차이무통계학의의(P＞0.05).척수급성압박손상후30 min,척수대체조가견손상부위종창,출혈,로극견뢰람(LFB)염색가견회질、백질출혈수종,중앙관파배,백질결구파배、불동정도탈수초병변.결론 척수급성압박손상후30 min즉존재회백질결구출혈、백질탈수초등병리변화,우척수손상국부응용신형갑리자통도조체제4-AP-3-MeOH가현저개선척수압박손상대서SSEP전도,촉진손상척수적신경전도공능회복.
Objective To evaluate the effects of 4-aminopyridine-3-methyl hydroxide (4-AP-3-MeOH) in rat's acute spinal cord injury.Methods A total of 12 adult male SD rats (250-300 g) were randomly divided into treatment (n =6) and control (n =6) groups.After compressing segment T11 of spinal cord for 30 min,the injured segment received 1 ml 4-AP-3-MeOH (100 μmol/ml) by topically application in treatment group while the control group received 1 ml saline.Somatosensory evoked potential (SSEP) was detected in both groups at pre-injury,30 min post-injury and post-dosing.Then Luxol fast blue (LFB) staining of target spinal segment was performed.Results In treatment group,the values of SSEP at pre-injury,30 min post-injury and post-dosing were 1.26 ± 0.35,0.03 ± 0.05 and 0.45 ± 0.19 μv respectively.Comparing SSEP of 30 min post-injury with post-dosing,the difference was statistically significant (P ＜0.01).While in control group,the values of SSEP at pre-injury,30 min post-injury and post-dosing were 1.05 ± 0.39,0.01 ± 0.02 and 0.02 ± 0.02 μv respectively.Comparing SSEP of 30 min post-injury with post-dosing,there was no statistical difference (P ＞ 0.05).After 30 min injury,there were swelling and bleeding of spinal cord.LFB staining showed that both gray and white matter had swelling and bleeding and central canal was destroyed with varying degrees of demyelination.Conclusion After 30 min of acute spinal cord injury,there are bleeding of gray and white matter with varying degrees of demyelination.Topical usage of K + blocker 4-AP-3-MeOH can effectively improve the conduction of SSEP after acute spinal cord injury in rats.