中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
20期
1559-1562
,共4页
王陆军%徐向升%冯国华%张学秀%王福生
王陸軍%徐嚮升%馮國華%張學秀%王福生
왕륙군%서향승%풍국화%장학수%왕복생
抑制因子,免疫%HIV-1%CD4阳性T淋巴细胞%CD8阳性T淋巴细胞
抑製因子,免疫%HIV-1%CD4暘性T淋巴細胞%CD8暘性T淋巴細胞
억제인자,면역%HIV-1%CD4양성T림파세포%CD8양성T림파세포
Suppressor factors,immunologic%HIV-1%CD4-positive T-lymphocytes%CD8-positive T-lymphocytes
目的 探讨慢性HIV-1感染者中CD4+和CD8+T细胞上共刺激分子CD160分子的表达及临床意义.方法 采集2010年1月至2011年12月解放军第三○二医院慢性HIV-1感染者23例(感染组)和20名健康人(对照组)的外周血,分离外周血单个核细胞,用直接免疫荧光染色方法标记并用流式细胞仪检测T细胞及Tn、Tcm、Tem和TemRA亚群上CD160表达的百分比及平均荧光强度(MFI),并将CD160的表达水平与患者CD4+T细胞计数和病毒载量做相关性分析.结果 感染组中CD4+和CD8+T细胞上CD160表达均显著高于对照组[表达率:(14.3±7.5)％比(3.9±2.2)％和(40.9±13.2)％比(12.9±6.6)％;MFI:26.4±11.0比12.4±2.9和52.5 ±17.4比18.2±5.0];且CD4+T细胞上CD160的表达与CD4+T细胞计数呈负相关(r=-0.550,P=0.010),和病毒载量呈正相关(r =0.522,P=0.015),但CD8+T细胞上CD160的表达与CD4+T细胞计数和病毒载量无相关性.感染组CD8+T细胞Tn、Tcm、Tem亚群上CD160表达均显著高于对照组(25.3±17.6比5.0±3.1,45.8±13.0比20.4 ±4.3,37.0±13.6比20.0±10.8),Tcm和Tem亚群上表达更高,且CD160+ CD8+T细胞亚群中Tn比例降低而Tcm比例升高.结论 在慢性HIV-1感染中共抑制分子CD160的升高,可能与CD4+和CD8+T细胞的功能耗竭有关,且CD160可能主要对记忆性CD8+T细胞产生影响.
目的 探討慢性HIV-1感染者中CD4+和CD8+T細胞上共刺激分子CD160分子的錶達及臨床意義.方法 採集2010年1月至2011年12月解放軍第三○二醫院慢性HIV-1感染者23例(感染組)和20名健康人(對照組)的外週血,分離外週血單箇覈細胞,用直接免疫熒光染色方法標記併用流式細胞儀檢測T細胞及Tn、Tcm、Tem和TemRA亞群上CD160錶達的百分比及平均熒光彊度(MFI),併將CD160的錶達水平與患者CD4+T細胞計數和病毒載量做相關性分析.結果 感染組中CD4+和CD8+T細胞上CD160錶達均顯著高于對照組[錶達率:(14.3±7.5)％比(3.9±2.2)％和(40.9±13.2)％比(12.9±6.6)％;MFI:26.4±11.0比12.4±2.9和52.5 ±17.4比18.2±5.0];且CD4+T細胞上CD160的錶達與CD4+T細胞計數呈負相關(r=-0.550,P=0.010),和病毒載量呈正相關(r =0.522,P=0.015),但CD8+T細胞上CD160的錶達與CD4+T細胞計數和病毒載量無相關性.感染組CD8+T細胞Tn、Tcm、Tem亞群上CD160錶達均顯著高于對照組(25.3±17.6比5.0±3.1,45.8±13.0比20.4 ±4.3,37.0±13.6比20.0±10.8),Tcm和Tem亞群上錶達更高,且CD160+ CD8+T細胞亞群中Tn比例降低而Tcm比例升高.結論 在慢性HIV-1感染中共抑製分子CD160的升高,可能與CD4+和CD8+T細胞的功能耗竭有關,且CD160可能主要對記憶性CD8+T細胞產生影響.
목적 탐토만성HIV-1감염자중CD4+화CD8+T세포상공자격분자CD160분자적표체급림상의의.방법 채집2010년1월지2011년12월해방군제삼○이의원만성HIV-1감염자23례(감염조)화20명건강인(대조조)적외주혈,분리외주혈단개핵세포,용직접면역형광염색방법표기병용류식세포의검측T세포급Tn、Tcm、Tem화TemRA아군상CD160표체적백분비급평균형광강도(MFI),병장CD160적표체수평여환자CD4+T세포계수화병독재량주상관성분석.결과 감염조중CD4+화CD8+T세포상CD160표체균현저고우대조조[표체솔:(14.3±7.5)％비(3.9±2.2)％화(40.9±13.2)％비(12.9±6.6)％;MFI:26.4±11.0비12.4±2.9화52.5 ±17.4비18.2±5.0];차CD4+T세포상CD160적표체여CD4+T세포계수정부상관(r=-0.550,P=0.010),화병독재량정정상관(r =0.522,P=0.015),단CD8+T세포상CD160적표체여CD4+T세포계수화병독재량무상관성.감염조CD8+T세포Tn、Tcm、Tem아군상CD160표체균현저고우대조조(25.3±17.6비5.0±3.1,45.8±13.0비20.4 ±4.3,37.0±13.6비20.0±10.8),Tcm화Tem아군상표체경고,차CD160+ CD8+T세포아군중Tn비례강저이Tcm비례승고.결론 재만성HIV-1감염중공억제분자CD160적승고,가능여CD4+화CD8+T세포적공능모갈유관,차CD160가능주요대기억성CD8+T세포산생영향.
Objective To explore the expression of CD160,a costimulator,on CD4 + and CD8 + T cells in chronic human immunodeficiency virus-1 (HIV-1) infected patients.Methods A total of 23 chronic HIV-1 infected patients were enrolled into 302 Hospital of People's Liberation Army from January 2010 to December 2011.Meanwhile,20 healthy individuals were selected as healthy controls (HC).Peripheral blood was collected,peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation and CD160 was stained with fluorescent antibody.The expressions of CD160 on CD4 + and CD8 + T cell subsets were detected by flow cytometry and its correlation with CD4 + T cell absolute number and HIV RNA viral load were analyzed.Results The expressions of CD160 on CD4 + and CD8 + T cell increased versus HC (on CD4 + T cells,percentage:(14.3 ± 7.5) ％ vs (3.9 ± 2.2) ％,median fluorescent intensity (MFI):26.4 ± 11.0 vs 12.4 ± 2.9 ; on CD8 + T cells,percentage:(40.9 ± 13.2) ％ vs (12.9 ± 6.6) ％,MFI:52.5 ± 17.4 vs 18.2 ± 5.0).Furthermore,the expression of CD160 on CD4 + T cells was negatively correlated with CD4 + T cell absolute number (r =-0.550,P =0.010) and positively with HIV RNA load (r =0.522,P =0.015).However,the expression of CD160 on CD8+ T cells was not correlated with CD4 + T cell absolute number or HIV RNA load.The expressions of CD160 on CD8 + T cell subsets Tn,Tcm and Tem in patients with chronic HIV-1 infection increased compared with HC (25.3 ± 17.6vs5.0±3.1,45.8 ±13.0 vs20.4±4.3,37.0±13.6 vs20.0 ±10.8).In addition,Tn subset decreased while Tcm subset increased in CD160 + CD8 + T cell subset distribution in patients with HIV-1 infection compared with HC subjects.Conclusion CD160 may contribute to the exhaustion of CD4 + and CD8 + T cells and influence the distribution of memory CD8 + T cell subsets.
