CAJ | 학술논문

目的建立北京地区汉族人群的ALT、AST、GGT和LDH的参考区间.方法按照CLSI文件C28-P3《医学实验室参考区间的定义、建立和确认》中的要求,借鉴IFCC多中心酶学参考区间研究模式及参考人群筛选标准,于2009年筛选卫生部北京医院20～60岁健康体检者315名,其中男132名,女183名,用于上述各种酶参考区间的研究.使用酶学参考物质保证本次研究结果的准确性.上述4种酶的检测方法均能溯源到IFCC的参考测量程序,其中检测ALT和AST的试剂中加入磷酸吡哆醛.结果ALT、AST和GGT需要按性别划分参考区间,ALT参考区间:8.2～ 50.8 U/L(女),12.7 ～71.8 U/L(男)；AST参考区间:15.0～36.7 U/L(女),16.6 ～51.1 U/L(男)；GGT参考区间:9.0 ～ 37.3 U/L(女),12.0 ～ 50.9 U/L(男).LDH无需按性别分组,参考区间为127 ～ 224 U/L.结论本研究建立了北京地区汉族人群4种酶的参考区间,其中ALT的参考上限高于同类研究.
목적 건립북경지구한족인군적ALT、AST、GGT화LDH적삼고구간.방법 안조CLSI문건C28-P3《의학실험실삼고구간적정의、건립화학인》중적요구,차감IFCC다중심매학삼고구간연구모식급삼고인군사선표준,우2009년사선위생부북경의원20～60세건강체검자315명,기중남132명,녀183명,용우상술각충매삼고구간적연구.사용매학삼고물질보증본차연구결과적준학성.상술4충매적검측방법균능소원도IFCC적삼고측량정서,기중검측ALT화AST적시제중가입린산필치철.결과ALT、AST화GGT수요안성별화분삼고구간,ALT삼고구간:8.2～ 50.8 U/L(녀),12.7 ～71.8 U/L(남)；AST삼고구간:15.0～36.7 U/L(녀),16.6 ～51.1 U/L(남)；GGT삼고구간:9.0 ～ 37.3 U/L(녀),12.0 ～ 50.9 U/L(남).LDH무수안성별분조,삼고구간위127 ～ 224 U/L.결론 본연구건립료북경지구한족인군4충매적삼고구간,기중ALT적삼고상한고우동류연구.
Objective To establish the reference intervals for ALT,AST,GGT and LDH among the Han nationality in Beijing.Methods The document C28-P3 issued by CLSI was a guideline about how to define,establish,and verify reference intervals in the clinical laboratory.IFCC had established multicenter enzymes reference intervals based on the guideline.Exclusion criteria were designed for screening candidate reference individual according to the document C28-P3 and the multicenter study's experience.Blood specimens were collected from 315 healthy individuals aged 20 to 60 years old,including 132 males and 183 females.Reference materials were used to ensure the accuracy of the test results of the four liver enzymes.The methods which used to test the four liver enzymes could be traced to the IFCC enzymes reference measure procedure,the reagent of ALT and AST included pyridoxal phosphate.Results There was statistically difference between males and females of the reference ranges for ALT, AST and GGT.Therefore,gender-specific reference intervals were established as ALT:8.2 -50.8 U/L (F),12.7 -71.8 U/L (M) ; AST:15.0 -36.7 U/L ( F),16.6 -51.1 U/L (M) ;GGT:9.0 -37.3 U/L (F),12.0 -50.9 U/L (M).For LDH,the reference interval was 127 -224 U/L,as no significant gender difference was found.Conclusions The reference intervals for the four liver enzymes based on the population of the Han nationality in Beijing are established.The upper reference limit for ALT in Beijing Han population is higher than that from other similar studies.