中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2011年
12期
1135-1138
,共4页
国汉邦%李红霞%满永%王抒%董军%陈文祥
國漢邦%李紅霞%滿永%王抒%董軍%陳文祥
국한방%리홍하%만영%왕서%동군%진문상
脂蛋白类,HDL%脂蛋白类,LDL%胆固醇,HDL%酯化%变异(遗传学)
脂蛋白類,HDL%脂蛋白類,LDL%膽固醇,HDL%酯化%變異(遺傳學)
지단백류,HDL%지단백류,LDL%담고순,HDL%지화%변이(유전학)
Lipoproteins,HDL%Lipoproteins,LDL%Cholesterol,HDL%Esterification%Variation(genetics)
目的 研究血清HDL亚类(HDL2-C、HDL3-C)、LDL亚类(LDLa-C、LDLb-C)、FERHDL和MERHDL的个体内和个体间的生物学变异(CVI、CVG).方法 选取2010年9-10月卫生部北京医院20名健康成年志愿者,男女各10名.每隔2周采集空腹静脉血1次,共采集4次,用超速离心-高效液相色谱(HPLC)法测定各脂蛋白亚类水平及FERHDL和MERHDL,计算其CVI和CVG,并给出各项指标的分析质量要求.结果 不同血脂指标的生物学变异不同,HDL3-C和HDL2-C的平均CVI分别为5.5％和7.2％,但两者的CVG差别较大,分别为8.7％和45.5％；LDLa-C和LDLb-C平均CVI分别为11.2％和18.7％；FERHDL和MERHDL的CVI分别为11.9％和12.3％,其中FERHDL的CVG (49.5％)大于MERHDL的CVG(30.6％).CVI存在较大个体差异.结论 本研究考察了血清脂蛋白亚类和HDL胆固醇酯化速率的生物学变异,可为这些血脂类危险因素的分析质量控制及心血管病危险分析奠定基础.
目的 研究血清HDL亞類(HDL2-C、HDL3-C)、LDL亞類(LDLa-C、LDLb-C)、FERHDL和MERHDL的箇體內和箇體間的生物學變異(CVI、CVG).方法 選取2010年9-10月衛生部北京醫院20名健康成年誌願者,男女各10名.每隔2週採集空腹靜脈血1次,共採集4次,用超速離心-高效液相色譜(HPLC)法測定各脂蛋白亞類水平及FERHDL和MERHDL,計算其CVI和CVG,併給齣各項指標的分析質量要求.結果 不同血脂指標的生物學變異不同,HDL3-C和HDL2-C的平均CVI分彆為5.5％和7.2％,但兩者的CVG差彆較大,分彆為8.7％和45.5％；LDLa-C和LDLb-C平均CVI分彆為11.2％和18.7％；FERHDL和MERHDL的CVI分彆為11.9％和12.3％,其中FERHDL的CVG (49.5％)大于MERHDL的CVG(30.6％).CVI存在較大箇體差異.結論 本研究攷察瞭血清脂蛋白亞類和HDL膽固醇酯化速率的生物學變異,可為這些血脂類危險因素的分析質量控製及心血管病危險分析奠定基礎.
목적 연구혈청HDL아류(HDL2-C、HDL3-C)、LDL아류(LDLa-C、LDLb-C)、FERHDL화MERHDL적개체내화개체간적생물학변이(CVI、CVG).방법 선취2010년9-10월위생부북경의원20명건강성년지원자,남녀각10명.매격2주채집공복정맥혈1차,공채집4차,용초속리심-고효액상색보(HPLC)법측정각지단백아류수평급FERHDL화MERHDL,계산기CVI화CVG,병급출각항지표적분석질량요구.결과 불동혈지지표적생물학변이불동,HDL3-C화HDL2-C적평균CVI분별위5.5％화7.2％,단량자적CVG차별교대,분별위8.7％화45.5％；LDLa-C화LDLb-C평균CVI분별위11.2％화18.7％；FERHDL화MERHDL적CVI분별위11.9％화12.3％,기중FERHDL적CVG (49.5％)대우MERHDL적CVG(30.6％).CVI존재교대개체차이.결론 본연구고찰료혈청지단백아류화HDL담고순지화속솔적생물학변이,가위저사혈지류위험인소적분석질량공제급심혈관병위험분석전정기출.
Objective To investigate the biological variations ( CVI,CVG ) of serum high-density (HDL2-C,HDL3-C ) and low-density (LDLa-C,LDLb-C ) lipoprotein subfractions and high-density lipoprotein cholesterol esterification rates (FERHDL and MERHDL ).Methods Twenty healthy volunteers,10 males and 10 females,were recruited for this study from September to October,2010.Blood was collected four times from each individual with a 2-week interval between each sampling.Serum lipoprotein subfraction cholesterol levels were measured by ultracentrifugation/HPLC,FERHDL and MERHDL were measured by HPLC.Within-subject ( CVI) and between-subject ( CVG.) biological variations and quality specifications for precision,bias and total error were calculated.Results The average CVI of this group were 5.5％ and 7.2％ for HDL3-C and HDL2-C,11.2％ and 18.7％ for LDLa-C and LDLb-C,11.95％ and 12.3％ for FERHDL and MERHDL,respectively.The CVG for HDL2-C was 45.5％,much higher than that of HDL3-C (8.7％),and FERHDL(49.5％ ) had a higher CVG than MERHDL (30.6％ ).For each analyte,there was a considerable variation of CVI among individuals.Conclusions Biological variations of lipoprotein subfractions,FERHDI and MERHDL have been estimated.These rsults will play an important role in quality specifications and cardiovascular disease risk assessment.
