中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2012年
11期
993-999
,共7页
沈胤晨%王建飞%杨红鹰%王方%石远凯%韩晓红
瀋胤晨%王建飛%楊紅鷹%王方%石遠凱%韓曉紅
침윤신%왕건비%양홍응%왕방%석원개%한효홍
结直肠肿瘤%原癌基因蛋白质B-raf%突变
結直腸腫瘤%原癌基因蛋白質B-raf%突變
결직장종류%원암기인단백질B-raf%돌변
Colorectal neoplasms%Proto-oncogene proteins B-raf%Mutation
目的 探讨中国结直肠癌患者鼠类肉瘤病毒癌基因同源物B1 (BRAF)基因突变与临床病理特征的关系及临床意义.方法 采用直接测序法检测2009年12月至2011年12月中国医学科学院肿瘤医院手术切除的676例结直肠癌患者组织标本中BRAF15及BRAF11外显子基因突变,并分析其与患者临床病理特征的关联性.结果 可有效检测的666例患者结直肠癌标本中的BRAF基因总突变率为4.35% (29/666),其中BRAF15(V600E)突变率为1.94% (13/669);BRAF11突变率为2.39% (16/670).结合临床病理特征分析,BRAF15突变与肿瘤分化程度相关(5.81%比1.46%),其分化程度越低,突变率越高(r=0.105,P=0.040).而突变率与患者年龄、性别、吸烟史、饮酒史、肿瘤原发部位、肿瘤形态、原发肿瘤分期(T分期/N分期)、肿瘤病理组织分型和是否有远处转移无相关性[r值分别为0.007、-0.018、-0.049、-0.023、-0.098、-0.038、0.040(0.034/0.059)、0.065、0.042,P均>0.05].在有、无饮酒史患者中BRAF11突变频率差异有统计学意义(6.02%比1.81%,r=0.093,P=0.035).然而,其突变率与患者年龄、性别、吸烟史、肿瘤原发部位、肿瘤形态、肿瘤分化程度、原发肿瘤分期(N分期)、肿瘤病理组织分型和是否有远处转移无相关性[r值分别为-0.004、0.047、0.020、0.042、0.029、0.040、0.006(-0.008)、0.008、0.030,P均>0.05].结论 我国结直肠癌患者BRAF15突变率随着肿瘤分化程度的降低逐渐升高;BRAF11在有饮酒史患者中突变率较高.
目的 探討中國結直腸癌患者鼠類肉瘤病毒癌基因同源物B1 (BRAF)基因突變與臨床病理特徵的關繫及臨床意義.方法 採用直接測序法檢測2009年12月至2011年12月中國醫學科學院腫瘤醫院手術切除的676例結直腸癌患者組織標本中BRAF15及BRAF11外顯子基因突變,併分析其與患者臨床病理特徵的關聯性.結果 可有效檢測的666例患者結直腸癌標本中的BRAF基因總突變率為4.35% (29/666),其中BRAF15(V600E)突變率為1.94% (13/669);BRAF11突變率為2.39% (16/670).結閤臨床病理特徵分析,BRAF15突變與腫瘤分化程度相關(5.81%比1.46%),其分化程度越低,突變率越高(r=0.105,P=0.040).而突變率與患者年齡、性彆、吸煙史、飲酒史、腫瘤原髮部位、腫瘤形態、原髮腫瘤分期(T分期/N分期)、腫瘤病理組織分型和是否有遠處轉移無相關性[r值分彆為0.007、-0.018、-0.049、-0.023、-0.098、-0.038、0.040(0.034/0.059)、0.065、0.042,P均>0.05].在有、無飲酒史患者中BRAF11突變頻率差異有統計學意義(6.02%比1.81%,r=0.093,P=0.035).然而,其突變率與患者年齡、性彆、吸煙史、腫瘤原髮部位、腫瘤形態、腫瘤分化程度、原髮腫瘤分期(N分期)、腫瘤病理組織分型和是否有遠處轉移無相關性[r值分彆為-0.004、0.047、0.020、0.042、0.029、0.040、0.006(-0.008)、0.008、0.030,P均>0.05].結論 我國結直腸癌患者BRAF15突變率隨著腫瘤分化程度的降低逐漸升高;BRAF11在有飲酒史患者中突變率較高.
목적 탐토중국결직장암환자서류육류병독암기인동원물B1 (BRAF)기인돌변여림상병리특정적관계급림상의의.방법 채용직접측서법검측2009년12월지2011년12월중국의학과학원종류의원수술절제적676례결직장암환자조직표본중BRAF15급BRAF11외현자기인돌변,병분석기여환자림상병리특정적관련성.결과 가유효검측적666례환자결직장암표본중적BRAF기인총돌변솔위4.35% (29/666),기중BRAF15(V600E)돌변솔위1.94% (13/669);BRAF11돌변솔위2.39% (16/670).결합림상병리특정분석,BRAF15돌변여종류분화정도상관(5.81%비1.46%),기분화정도월저,돌변솔월고(r=0.105,P=0.040).이돌변솔여환자년령、성별、흡연사、음주사、종류원발부위、종류형태、원발종류분기(T분기/N분기)、종류병리조직분형화시부유원처전이무상관성[r치분별위0.007、-0.018、-0.049、-0.023、-0.098、-0.038、0.040(0.034/0.059)、0.065、0.042,P균>0.05].재유、무음주사환자중BRAF11돌변빈솔차이유통계학의의(6.02%비1.81%,r=0.093,P=0.035).연이,기돌변솔여환자년령、성별、흡연사、종류원발부위、종류형태、종류분화정도、원발종류분기(N분기)、종류병리조직분형화시부유원처전이무상관성[r치분별위-0.004、0.047、0.020、0.042、0.029、0.040、0.006(-0.008)、0.008、0.030,P균>0.05].결론 아국결직장암환자BRAF15돌변솔수착종류분화정도적강저축점승고;BRAF11재유음주사환자중돌변솔교고.
Objective To determine the mutant status of BRAF gene in Chinese colorectal cancer (CRC) patients and analyze the association with clinicopathological parameters.Methods 676 CRC samples were collected in Cancer Institute/ Hospital,Chinese Academy of Medical Science from December 2009 to December 2011.The direct sequencing was conducted to detect mutations in the BRAF (exon 15 and exon 11).The correlation between mutant status with clinicopathological parameters were analyzed.Results Beside 10 colorectal cancer samples,among the 666 colorectal cancer patients 4.35% (29/666) of the tumors harbored a BRAF mutation,of which 1.94% (13/669) in exon 15 (V600E),2.39% (16/670) in exon 11.Statistical analysis revealed that BRAF15 mutations appeared to occur more frequently in poor-differentiation tumors than high or moderate-differentiation tumors (5.81% vs 1.46%,r =0.105,P=0.040).But BRAF15 mutations were not correlated with age,gender,smoking and drinking history,tumor site,tumor type,tumor(T/N) staging,histological type,or distant metastasis [r equals to 0.007,-0.018,-0.049,-0.023,-0.098,-0.038,0.040(0.034/0.059),0.065,0.042,respectively,P > 0.05] ; BRAF11 mutations appeared to occur more frequently in patients with drinking history (6.02%vs 1.81%,r =0.093,P =0.035).However,age,gender,smoking history,tumor site,tumor type,tumor differentiation,tumor(N) staging,histological type,or distant metastasis showed no significant correlation with this mutation [r equals to-0.004,0.047,0.020,0.042,0.029,0.040,0.006 (-0.008),0.008,0.030,respectively,P > 0.05].Conclusion A higher proportion of BRAF15 (V600E) mutations occurred in poor-differentiation tumors among the Chinese patients with CRC; BRAF11 mutations appeared more frequently in patients with drinking history.