CAJ | 학술논문

目的建立肌钙蛋白Ⅰ(Troponin Ⅰ)和肌酸激酶同工酶(CK-MB)儿童参考区间.方法 2011年8-12月收集上海儿童医学中心年龄7个月至12岁健康体检儿童385名,其中男223名,女162名.血液收集后离心,血清-70℃冷冻保存,使用贝克曼库尔特UniCel DxI 800免疫分析系统同一天集中测定结果.以非参数法计算99％参考区间.结果 Troponin Ⅰ共385例,不按年龄和性别划分的参考区间上限值为0.01μg/L.CK-MB按年龄划分的参考区间上限值为:A组(7个月～1岁)128例:13.41μg/L;B组(2～3岁)128例:9.35 μg/L;C组(4～12岁)129例:5.48 μg/L.结论确定了本院实验室健康儿童(7个月～12岁)Troponin Ⅰ和按年龄分组CK-MB的参考区间.在儿童心脏疾病中利用以上参考区间能够有效诊断与治疗早期心肌损伤.
목적 건립기개단백Ⅰ(Troponin Ⅰ)화기산격매동공매(CK-MB)인동삼고구간.방법 2011년8-12월수집상해인동의학중심년령7개월지12세건강체검인동385명,기중남223명,녀162명.혈액수집후리심,혈청-70℃냉동보존,사용패극만고이특UniCel DxI 800면역분석계통동일천집중측정결과.이비삼수법계산99％삼고구간.결과 Troponin Ⅰ공385례,불안년령화성별화분적삼고구간상한치위0.01μg/L.CK-MB안년령화분적삼고구간상한치위:A조(7개월～1세)128례:13.41μg/L;B조(2～3세)128례:9.35 μg/L;C조(4～12세)129례:5.48 μg/L.결론 학정료본원실험실건강인동(7개월～12세)Troponin Ⅰ화안년령분조CK-MB적삼고구간.재인동심장질병중이용이상삼고구간능구유효진단여치료조기심기손상.
Objective To establish the pediatric reference intervals for Troponin Ⅰ and Creatine kinase-MB (CK-MB).Methods Healthy children (223 boys and 162 girls) aged from 7 months to 12 years old were studied.Blood specimens were collected and centrifuged,all serum samples were kept refrigerated below-70 ℃.Troponin Ⅰ and CK-MB were measured on the UniCel DxI 800 immunoassay system (Beckman Coulter) on the same day.Nonparametric statistics was used to estimate the 99th percentile reference intervals.Results The children were divided into three groups (group A 128 cases; group B 128 cases;group C 129 cases) according to age.The upper reference limits for CK-MB were dependent of age,and the group A (7 months-1 years) was shown to be 13.41 μg/L,the group B (2-3 years) was shown to be 9.35 μg/L,the group C (4-12 years) was shown to be 5.48 μg/L.The upper reference limit for Troponin Ⅰ was independent of age or gender and shown to be 0.01 μg/L among 385 children.Conclusions Pediatric reference intervals for Troponin Ⅰ and CK-MB have been defined in healthy children at a relevant age group (7 months-12 years).It is effective for clinical diagnosis and treatment of myocardial damage for children by determining reference intervals for Troponin Ⅰ and CK-MB.