中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2013年
2期
146-150
,共5页
禹松林%董军%周伟燕%赵海建%杨睿悦%李红霞%张天娇%国汉邦%王抒
禹鬆林%董軍%週偉燕%趙海建%楊睿悅%李紅霞%張天嬌%國漢邦%王抒
우송림%동군%주위연%조해건%양예열%리홍하%장천교%국한방%왕서
色谱法,液相%串联质谱法%胆固醇酯类%脂肪酸类,ω3%心血管疾病
色譜法,液相%串聯質譜法%膽固醇酯類%脂肪痠類,ω3%心血管疾病
색보법,액상%천련질보법%담고순지류%지방산류,ω3%심혈관질병
Chromatography,liquid%Tandem mass spectrometry%Cholesteryl esters%Fatty acids,omega-3%Cardiovascular disease
目的 建立液相色谱串联质谱(liquid chromatography tandem mass spectrometry,LC-MS/MS)测定血清胆固醇酯n-3脂肪酸(CE-n-3 FA)的方法,以进行营养监测和心血管疾病危险分析.方法 本研究为方法学建立及评价.2012年1-8月征集111名北京地区健康志愿者(年龄19～72岁),其中男55名,女56名.建立各胆固醇酯(CEs)质谱测定条件,异丙醇提取血清CEs,稀释后用LC-MS/MS分析各种CEs,归一化法定量.评价新方法的精密度.用新建立的LC-MS/MS方法测定血清CE-n-3 FA含量,同时用试剂盒测定三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)和超敏C反应蛋白(hs-CRP).用Spearman相关分析CE-n-3FA与年龄、体质指数(BMI)、TG、HDL-C、LDL-C和hs-CRP的关系.结果 样本分析在5 min内完成,11种主要CEs分离良好；CE20:5和CE22:6平均批内CV分别为1.6％ ～ 2.3％和1.5％ ～2.3％,总CV分别为3.9％ ～7.6％和3.5％ ～ 7.4％,未见明显干扰因素.111名健康志愿者血清分析结果显示,CE20∶5和CE22∶6均呈正偏态分布(偏度分别为1.81和1.09；峰度分别为4.50和1.40),中位数分别为0.37％ (0.13％ ～ 1.26％)和0.50％ (0.29％ ～0.94％).CE20∶5与年龄、TG、TC、hs-CRP呈正相关(P＜0.05).结论 成功建立了LC-MS/MS测定血清CE-n-3 FA含量的方法,有望用于营养监测,CE-n-3 FA与心血管疾病的关系还有待进一步研究.
目的 建立液相色譜串聯質譜(liquid chromatography tandem mass spectrometry,LC-MS/MS)測定血清膽固醇酯n-3脂肪痠(CE-n-3 FA)的方法,以進行營養鑑測和心血管疾病危險分析.方法 本研究為方法學建立及評價.2012年1-8月徵集111名北京地區健康誌願者(年齡19～72歲),其中男55名,女56名.建立各膽固醇酯(CEs)質譜測定條件,異丙醇提取血清CEs,稀釋後用LC-MS/MS分析各種CEs,歸一化法定量.評價新方法的精密度.用新建立的LC-MS/MS方法測定血清CE-n-3 FA含量,同時用試劑盒測定三酰甘油(TG)、總膽固醇(TC)、高密度脂蛋白膽固醇(HDL-C)、低密度脂蛋白膽固醇(LDL-C)和超敏C反應蛋白(hs-CRP).用Spearman相關分析CE-n-3FA與年齡、體質指數(BMI)、TG、HDL-C、LDL-C和hs-CRP的關繫.結果 樣本分析在5 min內完成,11種主要CEs分離良好；CE20:5和CE22:6平均批內CV分彆為1.6％ ～ 2.3％和1.5％ ～2.3％,總CV分彆為3.9％ ～7.6％和3.5％ ～ 7.4％,未見明顯榦擾因素.111名健康誌願者血清分析結果顯示,CE20∶5和CE22∶6均呈正偏態分佈(偏度分彆為1.81和1.09；峰度分彆為4.50和1.40),中位數分彆為0.37％ (0.13％ ～ 1.26％)和0.50％ (0.29％ ～0.94％).CE20∶5與年齡、TG、TC、hs-CRP呈正相關(P＜0.05).結論 成功建立瞭LC-MS/MS測定血清CE-n-3 FA含量的方法,有望用于營養鑑測,CE-n-3 FA與心血管疾病的關繫還有待進一步研究.
목적 건립액상색보천련질보(liquid chromatography tandem mass spectrometry,LC-MS/MS)측정혈청담고순지n-3지방산(CE-n-3 FA)적방법,이진행영양감측화심혈관질병위험분석.방법 본연구위방법학건립급평개.2012년1-8월정집111명북경지구건강지원자(년령19～72세),기중남55명,녀56명.건립각담고순지(CEs)질보측정조건,이병순제취혈청CEs,희석후용LC-MS/MS분석각충CEs,귀일화법정량.평개신방법적정밀도.용신건립적LC-MS/MS방법측정혈청CE-n-3 FA함량,동시용시제합측정삼선감유(TG)、총담고순(TC)、고밀도지단백담고순(HDL-C)、저밀도지단백담고순(LDL-C)화초민C반응단백(hs-CRP).용Spearman상관분석CE-n-3FA여년령、체질지수(BMI)、TG、HDL-C、LDL-C화hs-CRP적관계.결과 양본분석재5 min내완성,11충주요CEs분리량호；CE20:5화CE22:6평균비내CV분별위1.6％ ～ 2.3％화1.5％ ～2.3％,총CV분별위3.9％ ～7.6％화3.5％ ～ 7.4％,미견명현간우인소.111명건강지원자혈청분석결과현시,CE20∶5화CE22∶6균정정편태분포(편도분별위1.81화1.09；봉도분별위4.50화1.40),중위수분별위0.37％ (0.13％ ～ 1.26％)화0.50％ (0.29％ ～0.94％).CE20∶5여년령、TG、TC、hs-CRP정정상관(P＜0.05).결론 성공건립료LC-MS/MS측정혈청CE-n-3 FA함량적방법,유망용우영양감측,CE-n-3 FA여심혈관질병적관계환유대진일보연구.
Objective To set up a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay to measure n-3 fatty acids in serum cholesteryl esters (CE-n-3 FA),to evaluate diatary intake and to do the risk assessment of cardiovascular diseases.Methods The mass spectrometry of each cholesteryl esters (CEs) were established using the CE standards.Serum CEs were extracted with isopropanol,diluted with mobile phase and then analyzed by LC-MS/MS.Serum CE-n-3 FA compositions were analyzed in 111healthy volunteers (19-72 years old,55 males and 56 females).Serum triglyceride (TG),total cholesterol (TC),high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C) and high sensitivity C reactive protein (hs-CRP) were analyzed by using the reagent kit.Results 11 major CEs were separated in 5 min.The inter-run CVs of CE20∶5 and CE22∶6 were 1.6％-2.3％ and 1.5％-2.3％,and total CVs were 3.9％-7.6％ and 3.5％-7.4％,respectively.No significant interference existed.Results of the 111 volunteers showed that the distribution of both CE20∶5 and CE22∶6 were approximately positively skewed (skewness =1.81,1.09; kurtosis =4.50,1.40) and the medians were 0.37％ (0.13％-1.26％) and 0.50％ 0.29％-0.94％),respectively.CE20∶5 significantly positively correlated with age,TC,TG and hs-CRP.Conclusions A method for the measurement of serum CE-n-3 FA by LC-MS/MS has been established.This method may be used in monitoring and evaluating dietary intake.Correlations of CE-n3 FA and CVD risk factors need to be further clarified.
