中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2013年
2期
156-160
,共5页
岳志红%菅广敏%贾玫%李珊珊
嶽誌紅%菅廣敏%賈玫%李珊珊
악지홍%관엄민%가매%리산산
冠心病%多态性,单核苷酸
冠心病%多態性,單覈苷痠
관심병%다태성,단핵감산
Coronary diseases%Polymorphism single nucleotide
目的 探讨脂蛋白相关磷脂酶A2的酶活性水平及I198T多态性是冠心病的危险因素.方法 采用病例对照研究,应用TaqMan-ARMS PCR方法,以2009年10月至2010年5月北京大学人民医院398例冠心病患者(CAD组)为病例组,与病例组年龄、性别相匹配的396例非冠心病对照组(NCAD组)为对照组,检测其PLA2G7的I198T基因多态性,并对Lp-PLA2的酶活性水平、胆固醇(CHO)、血糖(GLU)、三酰甘油(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、超敏C反应蛋白(Hs-CRP)、脂蛋白a Lp(a)进行测定和调查,运用独立样本t检验、卡方检验、方差分析、logistic分析对数据进行分析.结果 冠心病组的Lp-PLA2酶活性水平高于非冠心病对照组(31.51 nmol·ml-1·min-1 ＞21.31 nmol·ml-1·min-1),差异有统计学意义(F=16.40,P＜0.05)；校正传统冠心病危险因子[CHO,TG,hs-CRP,Lp(a),GLU]后,Lp-PLA2酶活性的最高四分位数与最低四分位数相比,CAD的比值比(OR)为7.50(95％CI:2.34 ～ 24.05)；Lp-PLA2酶活性水平在基因型Ⅱ最高(22.68 nmol· ml-1·min-1,P ＜0.05),基因型TT的最低(11.35 nmol·ml-1·min-1,P＜0.05),I198T点突变与冠心病的无明显关联(P＞0.05).结论 Lp-PLA2的酶活性水平在冠心病患者中显著升高,是冠心病的危险因子.I198T点突变与冠心病的无明显关联.
目的 探討脂蛋白相關燐脂酶A2的酶活性水平及I198T多態性是冠心病的危險因素.方法 採用病例對照研究,應用TaqMan-ARMS PCR方法,以2009年10月至2010年5月北京大學人民醫院398例冠心病患者(CAD組)為病例組,與病例組年齡、性彆相匹配的396例非冠心病對照組(NCAD組)為對照組,檢測其PLA2G7的I198T基因多態性,併對Lp-PLA2的酶活性水平、膽固醇(CHO)、血糖(GLU)、三酰甘油(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、超敏C反應蛋白(Hs-CRP)、脂蛋白a Lp(a)進行測定和調查,運用獨立樣本t檢驗、卡方檢驗、方差分析、logistic分析對數據進行分析.結果 冠心病組的Lp-PLA2酶活性水平高于非冠心病對照組(31.51 nmol·ml-1·min-1 ＞21.31 nmol·ml-1·min-1),差異有統計學意義(F=16.40,P＜0.05)；校正傳統冠心病危險因子[CHO,TG,hs-CRP,Lp(a),GLU]後,Lp-PLA2酶活性的最高四分位數與最低四分位數相比,CAD的比值比(OR)為7.50(95％CI:2.34 ～ 24.05)；Lp-PLA2酶活性水平在基因型Ⅱ最高(22.68 nmol· ml-1·min-1,P ＜0.05),基因型TT的最低(11.35 nmol·ml-1·min-1,P＜0.05),I198T點突變與冠心病的無明顯關聯(P＞0.05).結論 Lp-PLA2的酶活性水平在冠心病患者中顯著升高,是冠心病的危險因子.I198T點突變與冠心病的無明顯關聯.
목적 탐토지단백상관린지매A2적매활성수평급I198T다태성시관심병적위험인소.방법 채용병례대조연구,응용TaqMan-ARMS PCR방법,이2009년10월지2010년5월북경대학인민의원398례관심병환자(CAD조)위병례조,여병례조년령、성별상필배적396례비관심병대조조(NCAD조)위대조조,검측기PLA2G7적I198T기인다태성,병대Lp-PLA2적매활성수평、담고순(CHO)、혈당(GLU)、삼선감유(TG)、고밀도지단백(HDL)、저밀도지단백(LDL)、초민C반응단백(Hs-CRP)、지단백a Lp(a)진행측정화조사,운용독립양본t검험、잡방검험、방차분석、logistic분석대수거진행분석.결과 관심병조적Lp-PLA2매활성수평고우비관심병대조조(31.51 nmol·ml-1·min-1 ＞21.31 nmol·ml-1·min-1),차이유통계학의의(F=16.40,P＜0.05)；교정전통관심병위험인자[CHO,TG,hs-CRP,Lp(a),GLU]후,Lp-PLA2매활성적최고사분위수여최저사분위수상비,CAD적비치비(OR)위7.50(95％CI:2.34 ～ 24.05)；Lp-PLA2매활성수평재기인형Ⅱ최고(22.68 nmol· ml-1·min-1,P ＜0.05),기인형TT적최저(11.35 nmol·ml-1·min-1,P＜0.05),I198T점돌변여관심병적무명현관련(P＞0.05).결론 Lp-PLA2적매활성수평재관심병환자중현저승고,시관심병적위험인자.I198T점돌변여관심병적무명현관련.
Objective To investigate whether I198T gene polymorphisms and Lp-PLA2 activity were the risk factors of CAD.Methods A case-control study was conducted in 398 people with coronary heart disease and 396 controls whose ages and sex were matched with coronary heart disease from Peking University People's Hospital in October 2009 to May 2010.The Il98T gene polymorphisms were detected by the amplification refractory mutation system (ARMS-PCR) using TaqMan probe.Lp-PLA2 activity,CHO,GLU,TG,HDL,LDL,hs-CRP,Lp (a) were investigated at the same time.The data were analyzed by Independent-samples T Test,Chi-square test,One-Way ANOVA,Binary Logistic Regression.Results LpPLA2 activity was significant higer in CAD group than that in the control group (31.51 nmol · ml-1 · min-1＞21.31 nmol · ml-1 · min-1,F =16.40,P ＜0.05).Adjustment for various traditional cardiovascular risk factors,including ages,sex,CHO,TG,Hs-CRP,Lp(a),and GLU,quartiles of Lp-PLA2 activity were associated with risk of CVD with a OR of 7.5 (95％ CI:2.34-24.05) for comparison of the top to bottom quartile.Lp-PLA2 activity was the highest (22.68 nmol · ml-1 · min-1,P ＜ 0.05) in genotype Ⅱ and the lowest (11.35 nmol · ml-1 · min-1,P ＜ 0.05) in genotype TT,the association between I198T and coronary artery disease was not significant (P ＞ 0.05).Conclusions Lp-PLA2 activity was significantly higher in CAD group and was a risk factor for CAD.There was no significant association between I198T polymorphism and CAD.
