中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2013年
4期
313-317
,共5页
刘莹%李挺建%卢园园%王良兴%余方友
劉瑩%李挺建%盧園園%王良興%餘方友
류형%리정건%로완완%왕량흥%여방우
葡萄球菌,金黄色%细菌毒素类%外毒素类%杀白细胞素类%细菌蛋白质类
葡萄毬菌,金黃色%細菌毒素類%外毒素類%殺白細胞素類%細菌蛋白質類
포도구균,금황색%세균독소류%외독소류%살백세포소류%세균단백질류
Staphylococcus aureus%Bacterial toxins%Exotoxins%Leukocidins%Bacterial proteins
目的 研究不同标本分离的金黄色葡萄球菌临床分离株Panton-Valentine杀白细胞素(PVL)的表达及与agrA表达的相关性.方法 收集2003年1月至2010年12月4家医院从临床各种标本中分离的金黄色葡萄球菌,所有菌株均为非重复株.采用普通PCR和DNA测序检测PVL基因.采用实时荧光定量PCR检测lukSmRNA和agrAmRNA的量.结果 经过PCR扩增及测序共筛检出96株PVL阳性的金黄色葡萄球菌,其中58株为医院获得株,28株为社区获得株,10株由于临床资料不全不能区分；54株分离自血液标本,33株分离自脓液标本及9株分离自痰液标本；67株分离自成人的临床标本,29株分离自儿童的临床标本.分离自脓液标本和血液标本的菌株lukSmRNA的相对表达量的中位数分别为1.500和0.818,分离自脓液标本菌株的lukSmRNA的表达量明显高于分离自血液标本菌株的量(U=634,P=0.025)；分离自儿童和成人的菌株lukSmRNA的相对表达量的中位数分别为1.292和0.540,儿童分离株的lukSmRNA的表达量明显高于成人分离株的量(U=660,P=0.013);社区分离株和医院分离株lukSmRNA的相对表达量的中位数分别为1.034和0.536,社区获得株的lukSmRNA的表达量明显高于医院获得株的量(U=338,P=0.012).在总的分离株中,lukSmRNA与agrAmRNA的表达量存在正相关性(r=0.592,P＜0.01)；分离自脓液标本菌株的lukSmRNA与agrAmRNA相关系数较高(r=0.810,P ＜0.01),但分离自血液标本菌株的两者相关性系数较低(r=0.543,P ＜0.01).分离自儿童标本菌株的lukSmRNA与agrAmRNA量之间相关性更高,为0.804(P＜0.01),而分离自成人标本菌株的两者相关系数为0.476 (P＜0.01).社区获得株与医院获得株两者的相关系数分别为0.767(P＜0.0l)和0.556(P ＜0.01).结论 不同的感染类型lukS/F-PV基因的表达不同,agr系统可能对lukS/F-PV基因的表达具有正调节作用,尤其是在脓液和儿童分离株中发挥重要调节作用.(中华检验医学杂志,2013,36:313-317)
目的 研究不同標本分離的金黃色葡萄毬菌臨床分離株Panton-Valentine殺白細胞素(PVL)的錶達及與agrA錶達的相關性.方法 收集2003年1月至2010年12月4傢醫院從臨床各種標本中分離的金黃色葡萄毬菌,所有菌株均為非重複株.採用普通PCR和DNA測序檢測PVL基因.採用實時熒光定量PCR檢測lukSmRNA和agrAmRNA的量.結果 經過PCR擴增及測序共篩檢齣96株PVL暘性的金黃色葡萄毬菌,其中58株為醫院穫得株,28株為社區穫得株,10株由于臨床資料不全不能區分；54株分離自血液標本,33株分離自膿液標本及9株分離自痰液標本；67株分離自成人的臨床標本,29株分離自兒童的臨床標本.分離自膿液標本和血液標本的菌株lukSmRNA的相對錶達量的中位數分彆為1.500和0.818,分離自膿液標本菌株的lukSmRNA的錶達量明顯高于分離自血液標本菌株的量(U=634,P=0.025)；分離自兒童和成人的菌株lukSmRNA的相對錶達量的中位數分彆為1.292和0.540,兒童分離株的lukSmRNA的錶達量明顯高于成人分離株的量(U=660,P=0.013);社區分離株和醫院分離株lukSmRNA的相對錶達量的中位數分彆為1.034和0.536,社區穫得株的lukSmRNA的錶達量明顯高于醫院穫得株的量(U=338,P=0.012).在總的分離株中,lukSmRNA與agrAmRNA的錶達量存在正相關性(r=0.592,P＜0.01)；分離自膿液標本菌株的lukSmRNA與agrAmRNA相關繫數較高(r=0.810,P ＜0.01),但分離自血液標本菌株的兩者相關性繫數較低(r=0.543,P ＜0.01).分離自兒童標本菌株的lukSmRNA與agrAmRNA量之間相關性更高,為0.804(P＜0.01),而分離自成人標本菌株的兩者相關繫數為0.476 (P＜0.01).社區穫得株與醫院穫得株兩者的相關繫數分彆為0.767(P＜0.0l)和0.556(P ＜0.01).結論 不同的感染類型lukS/F-PV基因的錶達不同,agr繫統可能對lukS/F-PV基因的錶達具有正調節作用,尤其是在膿液和兒童分離株中髮揮重要調節作用.(中華檢驗醫學雜誌,2013,36:313-317)
목적 연구불동표본분리적금황색포도구균림상분리주Panton-Valentine살백세포소(PVL)적표체급여agrA표체적상관성.방법 수집2003년1월지2010년12월4가의원종림상각충표본중분리적금황색포도구균,소유균주균위비중복주.채용보통PCR화DNA측서검측PVL기인.채용실시형광정량PCR검측lukSmRNA화agrAmRNA적량.결과 경과PCR확증급측서공사검출96주PVL양성적금황색포도구균,기중58주위의원획득주,28주위사구획득주,10주유우림상자료불전불능구분；54주분리자혈액표본,33주분리자농액표본급9주분리자담액표본；67주분리자성인적림상표본,29주분리자인동적림상표본.분리자농액표본화혈액표본적균주lukSmRNA적상대표체량적중위수분별위1.500화0.818,분리자농액표본균주적lukSmRNA적표체량명현고우분리자혈액표본균주적량(U=634,P=0.025)；분리자인동화성인적균주lukSmRNA적상대표체량적중위수분별위1.292화0.540,인동분리주적lukSmRNA적표체량명현고우성인분리주적량(U=660,P=0.013);사구분리주화의원분리주lukSmRNA적상대표체량적중위수분별위1.034화0.536,사구획득주적lukSmRNA적표체량명현고우의원획득주적량(U=338,P=0.012).재총적분리주중,lukSmRNA여agrAmRNA적표체량존재정상관성(r=0.592,P＜0.01)；분리자농액표본균주적lukSmRNA여agrAmRNA상관계수교고(r=0.810,P ＜0.01),단분리자혈액표본균주적량자상관성계수교저(r=0.543,P ＜0.01).분리자인동표본균주적lukSmRNA여agrAmRNA량지간상관성경고,위0.804(P＜0.01),이분리자성인표본균주적량자상관계수위0.476 (P＜0.01).사구획득주여의원획득주량자적상관계수분별위0.767(P＜0.0l)화0.556(P ＜0.01).결론 불동적감염류형lukS/F-PV기인적표체불동,agr계통가능대lukS/F-PV기인적표체구유정조절작용,우기시재농액화인동분리주중발휘중요조절작용.(중화검험의학잡지,2013,36:313-317)
Objective To investigate the correlation between expression of Panton-Valentine leukocidin gene and accessory gene regulator among different clinical isolates of Staphylococcus aureus.Methods All non-duplicate Staphylococcus aureus clinical isolates were isolated from various clinical specimens of the patients at 4 hospitals from January 2003 to December 2010.Panton-Valentine leukocidin genes among Staphylococcus aureus clinical isolates were detected by PCR and DNA sequencing.The expressions of lukS-PV and agrA were determined by real-time PCR.Results Ninty-six S.aureus isolates including 58 hospital-acquired and 28 community-acquired isolates were positive for PVL genes,among which 54 from blood,33 from pus and 9 from sputum.Ten isolates cannot be classified due to lack of information.Sixty-seven and 29 PVL-positive isolates were isolated from the specimens of adults and children.The median relative quantities of lukSmRNA of the isolates from pus and blood were 1.500 and 0.818.The quantity of lukSmRNA among the isolates from pus was significantly higher than that from blood (U =634,P =0.025).The median relative quantities of lukSmRNA of the isolates from children and adults were 1.292 and 0.540,respectively.The quantity of lukSmRNA among the isolates from children was significantly higher than that from adults (U =660,P =0.013).The median relative quantities of lukSmRNA among community-acquired and hospital-acquired isolates were 1.034 and 0.536,respectively.The quantity of lukSmRNA among community-acquired isolates was significantly higher than that from hospital-acquired isolates (U =338,P =0.012).The correlation coefficients between lukSmRNA and agrAmRNA of total isolates,pus isolates and blood isolates were 0.592 (P ＜ 0.01),0.810 (P ＜ 0.0l) and 0.543 (P ＜0.01),respectively.While the correlation coefficients of those among the isolates from children and adults were 0.804 (P ＜ 0.01) and 0.476 (P ＜ 0.01).The correlation coefficients of those among the isolates from community-acquired and hospital-acquired isolates were 0.767 (P ＜ 0.01) and 0.556 (P＜0.01).Conclusions The quantity of lukSmRNA of Staphylococcus aureus isolates from pus was significantly higher than that from blood.The agr may have positive regulation effect on the expression of lukS/F-PV,especially among the isolates from pus and children.(Chin J Lab Med,2013,36:313-317)
