CAJ | 학술논문

目的体外检测阿司匹林、氟康唑单用或联合对生物膜白念珠菌的抑菌活性,评价两药联合的抗菌效果.方法分别测定阿司匹林和氟康唑对生物膜白念珠菌的MIC50值,采用分级抑制浓度指数检测阿司匹林与氟康唑之间的抗菌作用关系.并应用荧光定量PCR检测分析阿司匹林对生物膜白念珠菌表面蛋白ALS3及HWP1表达的影响.结果阿司匹林对生物膜白念珠菌标准株ATCC64550,临床分离株14215、15346、15538及16335的MICs0分别为＞1440 mg/L、＞1440 mg/L、1440 mg/L、720 mg/L及1440 mg/L,氟康唑对标准株及临床株生物膜白念珠菌的MIC50均＞64 mg/L.联合用药对氟康唑耐药株ATCC64550作用增强不明显,对4株临床分离株分级抑制浓度(FIC)指数分别为0.75、0.5、0.75、0.75,表现为协同或相加作用.在阿司匹林作用下,与白念珠菌生物膜形成相关的表面蛋白ALS3和HWP1表达明显下降.结论阿司匹林能抑制白念珠菌生物膜的形成,可能促进生物膜白念珠菌对氟康唑的敏感性.
목적 체외검측아사필림、불강서단용혹연합대생물막백념주균적억균활성,평개량약연합적항균효과.방법 분별측정아사필림화불강서대생물막백념주균적MIC50치,채용분급억제농도지수검측아사필림여불강서지간적항균작용관계.병응용형광정량PCR검측분석아사필림대생물막백념주균표면단백ALS3급HWP1표체적영향.결과 아사필림대생물막백념주균표준주ATCC64550,림상분리주14215、15346、15538급16335적MICs0분별위＞1440 mg/L、＞1440 mg/L、1440 mg/L、720 mg/L급1440 mg/L,불강서대표준주급림상주생물막백념주균적MIC50균＞64 mg/L.연합용약대불강서내약주ATCC64550작용증강불명현,대4주림상분리주분급억제농도(FIC)지수분별위0.75、0.5、0.75、0.75,표현위협동혹상가작용.재아사필림작용하,여백념주균생물막형성상관적표면단백ALS3화HWP1표체명현하강.결론 아사필림능억제백념주균생물막적형성,가능촉진생물막백념주균대불강서적민감성.
Objective To assess antifungal activity of aspirin and fluconazole administered alone or in combination against Candida albicans biofilms in vitro,and to evaluate the combined effect of these two drugs.Methods The MIC50 of aspirin and fluconzole against biofilm-associated adherent cells were determined respectively,then the efficacy of combinations of aspirin and fluconazole were evaluated by calculating the fractional inhibitory concentration (FIC) index.The influence of aspirin on the mRNA expression of gene ALS3,HWP1 in biofilm cells were analyzed by fluorescent quantitative PCR assay.Results The MIC50 of aspirin for ATCC64550,clinical strains 14215,15346,15538,16335 were ＞ 1440 mg/L,＞ 1440 mg/L,1440 mg/L,720 mg/L and 1440 mg/L respectively,the MIC50 of fluconazole for biofilms cells of all the strains were ＞ 64 mg/L.Aspirin did not enhance the antifungal effect of fluconazole against biofilm formed by ATCC64550,but synergistic and additive effects were found for the combination of aspirin and fluconazole to block the biofilm formation by clinical isolates (FIC index =0.75,0.5,0.75,0.75).Quantitative real-time PCR analysis showed aspirin could reduce the transcript level of ALS3 and HWP1.Conclusion Aspirin could inhibit C.albicans biofilm formation; it may increase the sensitivity of biofilm cells of C.albicans to fluconazole.