中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2013年
7期
630-633
,共4页
田正芹%刘红星%王芳%朱娟%张阳%陈雪%滕文%童春容%朱平
田正芹%劉紅星%王芳%硃娟%張暘%陳雪%滕文%童春容%硃平
전정근%류홍성%왕방%주연%장양%진설%등문%동춘용%주평
白血病,髓系,慢性%前体B细胞淋巴母细胞白血病淋巴瘤%Ikaros转录因子%突变
白血病,髓繫,慢性%前體B細胞淋巴母細胞白血病淋巴瘤%Ikaros轉錄因子%突變
백혈병,수계,만성%전체B세포림파모세포백혈병림파류%Ikaros전록인자%돌변
Leukemia,myelogenous,chronic,BCR-ABL positive%Precursor B-cell lymphoblastic leukemia-lymphoma%Ikaros transcription factor%Mutation
目的 探讨IKZF1基因变异与慢性髓细胞白血病急变的关系.方法 选取北京大学第一医院及陆道培血液肿瘤中心2009年6月至2011年10月就诊的212例白血病[156例急性淋巴细胞白血病(B-ALL)、20例急性髓性白血病(AML)、36例慢性髓细胞白血病(CML)]患者及10名健康对照者为研究对象.采用巢式PCR方法扩增患者初治或急变期标本中IKZF1基因cDNA,PCR后的产物进行电泳检测,对异常扩增片段进行基因测序分析,以确定IKZF1基因的不同亚型.结果 BALL中常有IKZFI异常表达,156例B-ALL中43例(27.6%)出现IKZF1异常表达.20例AML和10名健康对照者标本中均未检测到IKZF1异常表达.BCR-ABL1阳性B-ALL有IKZF1异常表达,38例中有30例(79.0%)增高,其中25例表达Ik6、2例表达Ik9、1例表达Ik7,2例IKZF1表达缺失.BCR-ABL1阴性的118例B-AL中,仅有13例(11.0%) IKZF1异常表达(均为Ik6).CML初治和慢性期标本中均未检测到IKZF1异常表达.CML发生B-ALL急变的12例患者中,7例(58.3%)有Ik6表达;而发生AML急变的CML标本中均无IKZFI异常表达.结论 IKZF1基因变异所致的2次打击是CML急变为B-ALL的一个重要因素.
目的 探討IKZF1基因變異與慢性髓細胞白血病急變的關繫.方法 選取北京大學第一醫院及陸道培血液腫瘤中心2009年6月至2011年10月就診的212例白血病[156例急性淋巴細胞白血病(B-ALL)、20例急性髓性白血病(AML)、36例慢性髓細胞白血病(CML)]患者及10名健康對照者為研究對象.採用巢式PCR方法擴增患者初治或急變期標本中IKZF1基因cDNA,PCR後的產物進行電泳檢測,對異常擴增片段進行基因測序分析,以確定IKZF1基因的不同亞型.結果 BALL中常有IKZFI異常錶達,156例B-ALL中43例(27.6%)齣現IKZF1異常錶達.20例AML和10名健康對照者標本中均未檢測到IKZF1異常錶達.BCR-ABL1暘性B-ALL有IKZF1異常錶達,38例中有30例(79.0%)增高,其中25例錶達Ik6、2例錶達Ik9、1例錶達Ik7,2例IKZF1錶達缺失.BCR-ABL1陰性的118例B-AL中,僅有13例(11.0%) IKZF1異常錶達(均為Ik6).CML初治和慢性期標本中均未檢測到IKZF1異常錶達.CML髮生B-ALL急變的12例患者中,7例(58.3%)有Ik6錶達;而髮生AML急變的CML標本中均無IKZFI異常錶達.結論 IKZF1基因變異所緻的2次打擊是CML急變為B-ALL的一箇重要因素.
목적 탐토IKZF1기인변이여만성수세포백혈병급변적관계.방법 선취북경대학제일의원급륙도배혈액종류중심2009년6월지2011년10월취진적212례백혈병[156례급성림파세포백혈병(B-ALL)、20례급성수성백혈병(AML)、36례만성수세포백혈병(CML)]환자급10명건강대조자위연구대상.채용소식PCR방법확증환자초치혹급변기표본중IKZF1기인cDNA,PCR후적산물진행전영검측,대이상확증편단진행기인측서분석,이학정IKZF1기인적불동아형.결과 BALL중상유IKZFI이상표체,156례B-ALL중43례(27.6%)출현IKZF1이상표체.20례AML화10명건강대조자표본중균미검측도IKZF1이상표체.BCR-ABL1양성B-ALL유IKZF1이상표체,38례중유30례(79.0%)증고,기중25례표체Ik6、2례표체Ik9、1례표체Ik7,2례IKZF1표체결실.BCR-ABL1음성적118례B-AL중,부유13례(11.0%) IKZF1이상표체(균위Ik6).CML초치화만성기표본중균미검측도IKZF1이상표체.CML발생B-ALL급변적12례환자중,7례(58.3%)유Ik6표체;이발생AML급변적CML표본중균무IKZFI이상표체.결론 IKZF1기인변이소치적2차타격시CML급변위B-ALL적일개중요인소.
Objective The new gene mutation as second hit is important for tumor progression,this paper explores the relationship between IKZF1 gene mutation and lymphoblast crisis of chronic myelogenous leukemia.Methods Two hundreds and twelve cases of leukemia patients were selected that treated in Department of Hematology,Peking University First Hospital and Lu Dao Pei Blood and Cancer Center,Hebei Yanda International Hospital from June 2009 to October 2011 as the research object [including 20 acute myelogenous leukemia (AML) cases,156 cases of B-acute lymphocytic leukemia (B-ALL),36 patients with chronic myelogenous leukemia (CML)],and 10 cases of healthy as controls.Firstly,nested PCR was used to amplify cDNA samples of IKZF1 gene from initial treatment patients or blast phase patients.And then the PCR products were detected by agarose gel electrophoresis.Different subtypes of IKZF1 gene could be obtained by sequencing abnormal amplified fragment.Results In 156 cases of B-ALL,43 cases (27.6%) were detected abnormal expression of IKZF1.Twenty cases of AML and 10 healthy control samples were not detected abnormal expression of IKZF1.Thirty cases (79.0%) out of 38 cases of BCR-ABLl-positive B-ALL were detected the IKZF1 abnormal expression and 25 cases were Ik6,2 cases were Ik9,1 cases were Ik7,2 cases were IKZF1 expression missing.In 118 cases of BCR-ABLl-negative B-ALL,13 cases (11.0%) were detected abnormal expression of IKZF1 (all were Ik6).Older patients with acute lymphocytic leukemia had a high rate of IKZF1 gene deletion.In 36 initial treatment and chronic phase CML cases were not detected abnormal expression of IKZF1.Among 12 cases that changed into B-ALL blast crisis from CML,7 cases (58.3%) expressed Ik6.IKZFI abnormal expression was not detected in patients which changed into AML blast crisis from CML.Contusion The IKZF1 geue mutation as second hit is an important factor of CML lymphoblast crisis.