中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2013年
8期
713-717
,共5页
宋海容%苏利沙%李洁平%李蕾花%刘红春
宋海容%囌利沙%李潔平%李蕾花%劉紅春
송해용%소리사%리길평%리뢰화%류홍춘
胰腺肿瘤%腺瘤状结肠息肉蛋白质%甲基化%基因,APC%启动区(遗传学)
胰腺腫瘤%腺瘤狀結腸息肉蛋白質%甲基化%基因,APC%啟動區(遺傳學)
이선종류%선류상결장식육단백질%갑기화%기인,APC%계동구(유전학)
Pancreatic neoplasms%Adenomatous polyposis coli protein%Methylation%Genes,APC%Promoter regions (genetics)
目的 探讨胰腺癌APC基因启动子的甲基化对表达的影响以及和临床病理资料的相关性.方法 病例对照研究.选取郑州大学第一附属医院2010年8月至2011年1月手术切除的胰腺癌标本60份,另取20份同期胰腺良性疾病组织作为对照组.使用甲基化特异性聚合酶链反应(MSP)、实时荧光定量PCR(RT-PCR)和蛋白质免疫印迹(Western blot)技术,检测60份胰腺癌、42份转移灶和20份胰腺良性疾病组织中APC启动子甲基化和基因表达水平,分析甲基化与APC表达及临床资料之间的关系.采用x2检验进行统计学分析.结果 在胰腺癌、转移灶和胰腺良性疾病组织中APC基因CG位点甲基化率分别是48.53％、46.67％和1.16％.胰腺癌和转移灶中APC基因启动子甲基化率明显高于对照组(x2=12.903、14.402,P均＜0.05).各组织中APC基因表达差异无统计学意义(P＞0.05).APC基因甲基化与临床分期相关(x2=6.801,P＜0.05),而与患者性别、年龄及肿瘤大小、组织学分级、有无转移无关(x2分别为0.727、1.311、0.372、0.148、0.017,P均＞0.05).结论 APC基因启动子甲基化与胰腺癌形成有密切关系,并且与胰腺癌的临床分期有关,但不影响其表达.
目的 探討胰腺癌APC基因啟動子的甲基化對錶達的影響以及和臨床病理資料的相關性.方法 病例對照研究.選取鄭州大學第一附屬醫院2010年8月至2011年1月手術切除的胰腺癌標本60份,另取20份同期胰腺良性疾病組織作為對照組.使用甲基化特異性聚閤酶鏈反應(MSP)、實時熒光定量PCR(RT-PCR)和蛋白質免疫印跡(Western blot)技術,檢測60份胰腺癌、42份轉移竈和20份胰腺良性疾病組織中APC啟動子甲基化和基因錶達水平,分析甲基化與APC錶達及臨床資料之間的關繫.採用x2檢驗進行統計學分析.結果 在胰腺癌、轉移竈和胰腺良性疾病組織中APC基因CG位點甲基化率分彆是48.53％、46.67％和1.16％.胰腺癌和轉移竈中APC基因啟動子甲基化率明顯高于對照組(x2=12.903、14.402,P均＜0.05).各組織中APC基因錶達差異無統計學意義(P＞0.05).APC基因甲基化與臨床分期相關(x2=6.801,P＜0.05),而與患者性彆、年齡及腫瘤大小、組織學分級、有無轉移無關(x2分彆為0.727、1.311、0.372、0.148、0.017,P均＞0.05).結論 APC基因啟動子甲基化與胰腺癌形成有密切關繫,併且與胰腺癌的臨床分期有關,但不影響其錶達.
목적 탐토이선암APC기인계동자적갑기화대표체적영향이급화림상병리자료적상관성.방법 병례대조연구.선취정주대학제일부속의원2010년8월지2011년1월수술절제적이선암표본60빈,령취20빈동기이선량성질병조직작위대조조.사용갑기화특이성취합매련반응(MSP)、실시형광정량PCR(RT-PCR)화단백질면역인적(Western blot)기술,검측60빈이선암、42빈전이조화20빈이선량성질병조직중APC계동자갑기화화기인표체수평,분석갑기화여APC표체급림상자료지간적관계.채용x2검험진행통계학분석.결과 재이선암、전이조화이선량성질병조직중APC기인CG위점갑기화솔분별시48.53％、46.67％화1.16％.이선암화전이조중APC기인계동자갑기화솔명현고우대조조(x2=12.903、14.402,P균＜0.05).각조직중APC기인표체차이무통계학의의(P＞0.05).APC기인갑기화여림상분기상관(x2=6.801,P＜0.05),이여환자성별、년령급종류대소、조직학분급、유무전이무관(x2분별위0.727、1.311、0.372、0.148、0.017,P균＞0.05).결론 APC기인계동자갑기화여이선암형성유밀절관계,병차여이선암적림상분기유관,단불영향기표체.
Objective To investigate the effect of methylation of the APC gene on expression and the correlation with clinical data in pancreatic cancer.Methods Sixty postoperative tissue samples with pancreatic cancer were collected in the First Affiliated Hospital of Zhengzhou University from August 2010 to January 2011,20 benign pancreatic disease tissues were collected as control groups.APC promoter methylation and gene expression levels were detected by Methylation Specific PCR (MSP),Real Time PCR (RT-PCR) and Western blot in 60 pancreatic carcinoma,42 metastasis and 20 benign pancreatic disease tissues,then analyze the relation between methylation of the APC gene and the clinical data.Results APC promoter methlation was observed 48.53％,46.67％ and 1.16％ in pancreatic carcinoma,metastasis and benign pancreatic disease tissue,respectively.Methylation of APC in pancreatic carcinoma and metastasis increased significantly compared with control tissues (x2 =12.903,14.402; P ＜ 0.05).There were no statistically significant differences of APC expression in these tissues (P ＞ 0.05).There was a significant correlation between methylation of APC and clinicopathological stage (x2 =6.801,P ＜ 0.05),but no correlation with gender,age,tumor size,histological grade and metastasis (x2 =0.727,1.311,0.372,0.148,0.017 ; P ＞ 0.05).Conclusion The methylation of APC gene is closely related with pancreatic carcinoma inogenesis and the clinicopathological stage,but do not effect the expression of APC in tissues.
