CAJ | 학술논문

目的探讨N J001特异性抗原在肺腺癌患者组织中的表达,及其与各临床特征之间的关系.方法病例对照研究.收集南京医科大学第一附属医院113份肺腺癌和46份肺部良性肿瘤组织标本,应用免疫组织化学方法检测其中N J001特异性抗原的表达,并用半定量评分法判定结果,比较该抗原的表达与肺腺癌临床病理参数之间的关系,并分析其与预后的关系.采用x2检验或Fisher确切概率法进行统计分析.结果 113份肺腺癌组织NJ001特异性抗原的阳性表达率为84.07％,高于肺部良性肿瘤组的8.70％,差异有统计学意义(x2=79.050,P＜0.01).中、低分化肺腺癌的N J001特异性抗原的阳性表达率89.41％,高于高分化者的63.16％,差异有统计学意义(Fisher确切概率法,P =0.009)；同时,有淋巴结转移的患者NJ001特异性抗原阳性表达率为91.53％,高于无淋巴结转移者的75.51％,(x2 =5.177,P＜0.05).Kaplan-Meier生存分析显示,NJ001特异性抗原表达程度越高,患者生存率越低；Cox比例风险模型分析提示,NJ001特异性抗原高表达是影响肺腺癌患者术后总体生存时间的独立危险因素.结论肺腺癌细胞NJ001特异性抗原的表达与肺腺癌的分化程度及淋巴结转移密切相关,有望成为肺腺癌患者预后的新指标.
목적 탐토N J001특이성항원재폐선암환자조직중적표체,급기여각림상특정지간적관계.방법 병례대조연구.수집남경의과대학제일부속의원113빈폐선암화46빈폐부량성종류조직표본,응용면역조직화학방법검측기중N J001특이성항원적표체,병용반정량평분법판정결과,비교해항원적표체여폐선암림상병리삼수지간적관계,병분석기여예후적관계.채용x2검험혹Fisher학절개솔법진행통계분석.결과 113빈폐선암조직NJ001특이성항원적양성표체솔위84.07％,고우폐부량성종류조적8.70％,차이유통계학의의(x2=79.050,P＜0.01).중、저분화폐선암적N J001특이성항원적양성표체솔89.41％,고우고분화자적63.16％,차이유통계학의의(Fisher학절개솔법,P =0.009)；동시,유림파결전이적환자NJ001특이성항원양성표체솔위91.53％,고우무림파결전이자적75.51％,(x2 =5.177,P＜0.05).Kaplan-Meier생존분석현시,NJ001특이성항원표체정도월고,환자생존솔월저；Cox비례풍험모형분석제시,NJ001특이성항원고표체시영향폐선암환자술후총체생존시간적독립위험인소.결론 폐선암세포NJ001특이성항원적표체여폐선암적분화정도급림파결전이밀절상관,유망성위폐선암환자예후적신지표.
Objective To evaluate the expression of NJ001 specific antigen in lung adenocarcinoma,and the relationship between the antigen and clinicopathological features.Methods A case-control study was conducted.Tumor tissues of 113 lung adenocarcinoma and 46 benign lung disease were collected from the First Affiliated Hospital of Nanjing Medical University.The expression of NJ001 specific antigen was examined by means of immunohistochemistry,and the results were scored by semiquantitative analysis.Then the associations of the antigen expression with several clinicopathologic features and the potential value in prognosis were assessed.Statistical analysis was evaluated by chi-square test or Fisher exact test.Results The positive rate of N J001 specific antigen was 84.70％ in 113 lung adenocarcinoma,which was higher than 8.70％ in benign lung disease,and there were significant differences between the two groups (x2 =79.050,P ＜ 0.01).In lung adenocarcinoma of poor and moderate differentiation,the positive rate of NJ001 specific antigen was 89.41％,and it was significantly higher than 63.16％ in well differentiation patients (P =0.009) ; Meanwhile,the positive rate in patients with lymph nodes metastasis was 91.53％,which was markedly higher than 75.51％ in those without lymph nodes metastasis (x2 =5.1 77,P ＜ 0.05).Kaplan-Meier survival analysis showed that the higher level of the specific antigen patients expressed,the lower survival rate they acquired.Cox proportional hazards model revealed that high expression of NJ001specific antigen was an independent risk factor for post-operation overall survival of lung adenocarcinoma patients.Conclusions The expression of NJ001 specific antigen is closely correlated with tumor differentiation degree and lymph node metastasis in lung adenocarcinoma patients,and it promises to be a novel marker for prognosis of lung adenocarcinoma patients.