CAJ | 학술논문

目的了解我国石家庄地区艰难梭菌临床分离株的多位点序列分型(MLST)情况.方法回顾性研究.收集2010年7月至2011年12月石家庄市两家医院成人腹泻患者分离的33株艰难梭菌,并对其进行PCR毒素基因检测、PCR核糖体分型和MLST.采用START2系统进行统计学分析.结果所分离的33株艰难梭菌中共检出10种ST型别(ST2/3/4/35/37/53/54/55/102/172)和12种核糖体型别,其中ST37/S Ⅰ(9/33,占27.3％)和ST54/SⅢ(8/33,占24.2％)是该地区流行的主要型别.ST172是新发现的ST型,已被MLST数据库收录.系统发育分析显示,其中6个ST型(ST2、ST3、ST4、ST53、ST54、ST102)位于一个较大组别,而ST35、ST37、ST55、ST172为零散型别.结论在石家庄地区医院内成人腹泻患者分离的33株艰难梭菌以ST37和ST54型为主,并且医院间无明显的同源性.
목적 료해아국석가장지구간난사균림상분리주적다위점서렬분형(MLST)정황.방법 회고성연구.수집2010년7월지2011년12월석가장시량가의원성인복사환자분리적33주간난사균,병대기진행PCR독소기인검측、PCR핵당체분형화MLST.채용START2계통진행통계학분석.결과 소분리적33주간난사균중공검출10충ST형별(ST2/3/4/35/37/53/54/55/102/172)화12충핵당체형별,기중ST37/S Ⅰ(9/33,점27.3％)화ST54/SⅢ(8/33,점24.2％)시해지구류행적주요형별.ST172시신발현적ST형,이피MLST수거고수록.계통발육분석현시,기중6개ST형(ST2、ST3、ST4、ST53、ST54、ST102)위우일개교대조별,이ST35、ST37、ST55、ST172위령산형별.결론 재석가장지구의원내성인복사환자분리적33주간난사균이ST37화ST54형위주,병차의원간무명현적동원성.
Objective To investigate the multilocus sequence typing (MLST) for clinical Clostridium difficile isolates in Shijiazhuang of China.Methods A laboratory-based study was carried out retrospectively to characterize 33 C.difficile strains isolated from diarrhea patients during July 2010 to December 2011 according to toxin types,PCR ribotyping and MLST.START2 system was used to analyze the results.Results Ten ST types (ST 2/3/4/35/37/53/54/55/102/172) and 12 PCR ribotyping were identified in 33 C.difficile strains.The most prevalent ST types and PCR ribotyping were ST37/S Ⅰ (9/33,27.3％) and ST54/SⅢ (8/33,24.2％).ST172 was a new ST type reported firstly in the world,and has been included in the MLST database.Phylogenetic analysis also revealed that 6 STs (ST2,ST3,ST4,ST53,ST54,ST102) were placed in one large group.Genotypes ST35,ST37,ST55,ST172 were considered as singletons.Conclusions The most common ST types for 33 clinical C.difficile strains isolated from Shijiazhuang were ST37 and ST54.No significant homology was found between ST types and hospitals.