中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2013年
11期
1013-1017
,共5页
周伟燕%张传宝%董军%王抒%张江涛%国汉邦%李红霞%胡翠华%赵海舰
週偉燕%張傳寶%董軍%王抒%張江濤%國漢邦%李紅霞%鬍翠華%趙海艦
주위연%장전보%동군%왕서%장강도%국한방%리홍하%호취화%조해함
胆固醇%甘油三酯类%胆固醇,HDL%胆固醇,LDL%参考标准
膽固醇%甘油三酯類%膽固醇,HDL%膽固醇,LDL%參攷標準
담고순%감유삼지류%담고순,HDL%담고순,LDL%삼고표준
Cholesterol%Triglycerides%Cholesterol,HDL%Cholesterol,LDL%Reference standards
目的 研制血清总胆固醇(TC)、总甘油(TTG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)冰冻血清标准物质.方法 实验性基础研究.2008年11月在卫生部北京医院募集年龄20～50岁的健康志愿者28名,男14名,女14名,采集志愿者血清,根据TC的浓度制备3种不同水平的混合血清池.用常规方法进行均匀性和同步稳定性研究.用同位素稀释液相色谱串联质谱(ID-LC/MS/MS)和高效液相色谱(HPLC)法为TC定值,HPLC、ID-LC/MS/MS和同位素稀释气相色谱质谱(ID-GC/MS)法为TTG定值,DCM法和超速离心/HPLC法分别为HDL-C和LDL-C定值.计算各检测项目的不确定度.结果 均匀性数据的方差分析结果显示3种血清池的P值均大于0.05,说明均匀性良好.同步稳定性研究结果显示3种物质在-70 ℃保存至少可稳定1年.3种血清的定值结果(标准值±扩展不确定度,mmoL/L)为:TC分别为6.798±0.075,4.470±0.050,3.221±0.042;TTG分别为1.327±0.024,0.824±0.017,0.659±0.014;HDL-C分别为1.640±0.028,1.652±0.027,1.184±0.022;LDL-C分别为4.577±0.097,2.492±0.072,1.788±0.041.结论 本研究成功研制了TC、TTG、HDL-C和LDL-C冰冻血清标准物质.
目的 研製血清總膽固醇(TC)、總甘油(TTG)、高密度脂蛋白膽固醇(HDL-C)和低密度脂蛋白膽固醇(LDL-C)冰凍血清標準物質.方法 實驗性基礎研究.2008年11月在衛生部北京醫院募集年齡20～50歲的健康誌願者28名,男14名,女14名,採集誌願者血清,根據TC的濃度製備3種不同水平的混閤血清池.用常規方法進行均勻性和同步穩定性研究.用同位素稀釋液相色譜串聯質譜(ID-LC/MS/MS)和高效液相色譜(HPLC)法為TC定值,HPLC、ID-LC/MS/MS和同位素稀釋氣相色譜質譜(ID-GC/MS)法為TTG定值,DCM法和超速離心/HPLC法分彆為HDL-C和LDL-C定值.計算各檢測項目的不確定度.結果 均勻性數據的方差分析結果顯示3種血清池的P值均大于0.05,說明均勻性良好.同步穩定性研究結果顯示3種物質在-70 ℃保存至少可穩定1年.3種血清的定值結果(標準值±擴展不確定度,mmoL/L)為:TC分彆為6.798±0.075,4.470±0.050,3.221±0.042;TTG分彆為1.327±0.024,0.824±0.017,0.659±0.014;HDL-C分彆為1.640±0.028,1.652±0.027,1.184±0.022;LDL-C分彆為4.577±0.097,2.492±0.072,1.788±0.041.結論 本研究成功研製瞭TC、TTG、HDL-C和LDL-C冰凍血清標準物質.
목적 연제혈청총담고순(TC)、총감유(TTG)、고밀도지단백담고순(HDL-C)화저밀도지단백담고순(LDL-C)빙동혈청표준물질.방법 실험성기출연구.2008년11월재위생부북경의원모집년령20～50세적건강지원자28명,남14명,녀14명,채집지원자혈청,근거TC적농도제비3충불동수평적혼합혈청지.용상규방법진행균균성화동보은정성연구.용동위소희석액상색보천련질보(ID-LC/MS/MS)화고효액상색보(HPLC)법위TC정치,HPLC、ID-LC/MS/MS화동위소희석기상색보질보(ID-GC/MS)법위TTG정치,DCM법화초속리심/HPLC법분별위HDL-C화LDL-C정치.계산각검측항목적불학정도.결과 균균성수거적방차분석결과현시3충혈청지적P치균대우0.05,설명균균성량호.동보은정성연구결과현시3충물질재-70 ℃보존지소가은정1년.3충혈청적정치결과(표준치±확전불학정도,mmoL/L)위:TC분별위6.798±0.075,4.470±0.050,3.221±0.042;TTG분별위1.327±0.024,0.824±0.017,0.659±0.014;HDL-C분별위1.640±0.028,1.652±0.027,1.184±0.022;LDL-C분별위4.577±0.097,2.492±0.072,1.788±0.041.결론 본연구성공연제료TC、TTG、HDL-C화LDL-C빙동혈청표준물질.
Objective To prepare serum reference materials for total cholesterol (TC),total glycerol (TTG),high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C).Methods Individual blood samples were collected from 28 healthy donors (14 males and 14females) aged from 20 to 50 years old in November 2008 in Beijing Hospital,and the sera were separated and mixed into 3 serum pools according to cholesterol levels.The materials were tested for homogeneity and stability using routine methods.The methods of isotope dilution liquid chromatography tandem mass spectrometry (ID-LC/MS/MS) and high performance liquid chromatography (HPLC) were used to determine the concentration of TC.HPLC,ID-LC/MS/MS and isotope dilution gas chromatography mass spectrometry (ID-GC/MS) methods were used for TTG.The values of HDL-C and LDL-C were assigned by the designated comparison method and ultracentrifugation/HPLC method,respectively.Results The materials were homogeneous and were tested to be stable for at least 1 year at-70 ℃ using the isochronous stability study.The certified values (reference value ± expanded uncertainty) of the 3 materials were:TC,6.798 ± 0.075,4.470 ± 0.050,3.221 ± 0.042 ; TTG,1.327 ± 0.024,0.824 ± 0.017,0.659 ± 0.014 ; HDL-C,1.640 ± 0.028,1.652 ± 0.027,1.184 ± 0.022 ; LDL-C,4.577 ± 0.097,2.492 ± 0.072,1.788 ± 0.041.Conclusion Certified reference materials for serum TC,TTG,HDL-C and LDL-C have been prepared.
