CAJ | 학술논문

目的评价基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)对醋酸钙鲍曼不动杆菌复合群的鉴定价值,了解浙江大学医学院附属第二医院醋酸钙鲍曼不动杆菌复合群的菌种分布情况.方法回顾性选取2012年1至7月我院临床分离的502株醋酸钙鲍曼不动杆菌复合群非重复菌株,用MALDI-TOF MS进行再鉴定,同时用16S-23S rRNA基因间隔区序列分析来确定所有菌株的菌种,比较二者鉴定结果的差异.结果 502株醋酸钙鲍曼不动杆菌复合群用MALDI-TOF MS再鉴定,包括鲍曼不动杆菌431株(85.9％),皮特不动杆菌68株(13.5％),醋酸钙不动杆菌3株(0.6％);对502株醋酸钙鲍曼不动杆菌复合群16S-23S rRNA基因间隔区序列分析结果显示:403株为鲍曼不动杆菌(80.3％),68株为皮特不动杆菌(13.5％),28株为医院不动杆菌(5.6％),3株为醋酸钙不动杆菌(0.6％).与16S-23S rRNA基因间隔区序列分析相比较,MALDI-TOF MS错误地将所有28株医院不动杆菌鉴定为鲍曼不动杆菌,其余菌株的鉴定结果完全一致.结论 MALDI-TOF MS可将醋酸钙鲍曼不动杆菌复合群菌株准确鉴定到种,具有操作简便、快速、成本低,重复性好的优点.MALDI-TOF MS有望在未来成为日常临床微生物鉴定的理想工具.
목적 평개기질보조격광해흡/전리비행시간질보(MALDI-TOF MS)대작산개포만불동간균복합군적감정개치,료해절강대학의학원부속제이의원작산개포만불동간균복합군적균충분포정황.방법 회고성선취2012년1지7월아원림상분리적502주작산개포만불동간균복합군비중복균주,용MALDI-TOF MS진행재감정,동시용16S-23S rRNA기인간격구서렬분석래학정소유균주적균충,비교이자감정결과적차이.결과 502주작산개포만불동간균복합군용MALDI-TOF MS재감정,포괄포만불동간균431주(85.9％),피특불동간균68주(13.5％),작산개불동간균3주(0.6％);대502주작산개포만불동간균복합군16S-23S rRNA기인간격구서렬분석결과현시:403주위포만불동간균(80.3％),68주위피특불동간균(13.5％),28주위의원불동간균(5.6％),3주위작산개불동간균(0.6％).여16S-23S rRNA기인간격구서렬분석상비교,MALDI-TOF MS착오지장소유28주의원불동간균감정위포만불동간균,기여균주적감정결과완전일치.결론 MALDI-TOF MS가장작산개포만불동간균복합군균주준학감정도충,구유조작간편、쾌속、성본저,중복성호적우점.MALDI-TOF MS유망재미래성위일상림상미생물감정적이상공구.
Objective To evaluate the ability of matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF MS) in identifying species of Acinetobacter calcoaceticus-Acinetobacter baumannii complex,and investigate the species distribution of Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated in our hospital.Methods A total of 502 nonduplicate clinical isolates of Acinetobacter calcoaceticus-Acinetobacter baumannii complex were retrospectively collected from the second affiliated hospital of Zhejiang University between January 2012 and July 2012.All strains were re-identified by MALDI-TOF MS and were also verified by sequence analysis of 16S-23S rRNA gene spacer region.Results Among all the 502 strains of Acinetobacter calcoaceticus-Acinetobacter baumannii complex,identificaion results provided by MALDI-TOF MS were A.baumannii (431,85.9％),A.pittii (68,13.5％),A.calcoaceticus (3,0.6％).Sequence analysis of 16S-23S rRNA gene spacer region was used to identify all the 502 strains:403 (80.3％) were identified as A.baumannii,68 (13.5％) as A.pittii,28 (5.6％) as A.nosocomialis and 3 (0.6％) as A.calcoaceticus.MALDI-TOF MS correctly identified all the strains but erroneously identified all 28 strains of A.nosocomialis as A.baumannii,compared with sequence analysis of 16S-23S rRNA gene spacer region.Conclusions MALDI-TOF MS can be used as a fast,simple,reliable and excellently reproducible method to identify members of Acinetobacter calcoaceticus-Acinetobacter baumannii complex at low costs.MALDI-TOF MS is expected to be an ideal technique for routine clinical microbiology testing in the future.