中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2014年
4期
266-269
,共4页
曾艳%范佳鸣%张丽芳%钱飞燕%许平%姜玮贇
曾豔%範佳鳴%張麗芳%錢飛燕%許平%薑瑋贇
증염%범가명%장려방%전비연%허평%강위빈
原位杂交,荧光%产前诊断
原位雜交,熒光%產前診斷
원위잡교,형광%산전진단
In situ hybridization,fluorescence%Prenatal diagnosis
目的 对荧光原位杂交方法进行改良,探讨一种更简便高效的杂交方法.方法 采用对照实验收集2012至2013年绍兴市妇幼保健院36份产前诊断绒毛标本,将标本制片后,使用18/X/Y、13/21探针,分别用改良三步法和经典法进行杂交,观察杂交率和荧光信号,并与传统培养结果进行配对t检验.结果 共检测72个杂交区,21/13探针三步法杂交率和杂交信号均明显高于经典法(杂交率99.72％ ±0.42％和85.90％±4.15％,t=20.4,P＜0.01;杂交信号2.58 ±0.50和1.52±0.55,t=7.53,P＜0.01),三步法18/X/Y探针杂效率与信号与经典法差异无统计学意义(杂效率99.57％ ±0.53％和99.70％ ±0.42％,t=1.30,P＞0.05;杂交信号2.22 ±0.42和2.36 ±0.48,t=1.57,P＞0.05);经典法与三步法的荧光原位杂交结果与传统培养结果符合率为100％(36/36).结论 本研究中的改良三步法操作简便,步骤简单、实验效率高、环保,但也存在18/X/Y信号偏散的情况,有待于进一步摸索.
目的 對熒光原位雜交方法進行改良,探討一種更簡便高效的雜交方法.方法 採用對照實驗收集2012至2013年紹興市婦幼保健院36份產前診斷絨毛標本,將標本製片後,使用18/X/Y、13/21探針,分彆用改良三步法和經典法進行雜交,觀察雜交率和熒光信號,併與傳統培養結果進行配對t檢驗.結果 共檢測72箇雜交區,21/13探針三步法雜交率和雜交信號均明顯高于經典法(雜交率99.72％ ±0.42％和85.90％±4.15％,t=20.4,P＜0.01;雜交信號2.58 ±0.50和1.52±0.55,t=7.53,P＜0.01),三步法18/X/Y探針雜效率與信號與經典法差異無統計學意義(雜效率99.57％ ±0.53％和99.70％ ±0.42％,t=1.30,P＞0.05;雜交信號2.22 ±0.42和2.36 ±0.48,t=1.57,P＞0.05);經典法與三步法的熒光原位雜交結果與傳統培養結果符閤率為100％(36/36).結論 本研究中的改良三步法操作簡便,步驟簡單、實驗效率高、環保,但也存在18/X/Y信號偏散的情況,有待于進一步摸索.
목적 대형광원위잡교방법진행개량,탐토일충경간편고효적잡교방법.방법 채용대조실험수집2012지2013년소흥시부유보건원36빈산전진단융모표본,장표본제편후,사용18/X/Y、13/21탐침,분별용개량삼보법화경전법진행잡교,관찰잡교솔화형광신호,병여전통배양결과진행배대t검험.결과 공검측72개잡교구,21/13탐침삼보법잡교솔화잡교신호균명현고우경전법(잡교솔99.72％ ±0.42％화85.90％±4.15％,t=20.4,P＜0.01;잡교신호2.58 ±0.50화1.52±0.55,t=7.53,P＜0.01),삼보법18/X/Y탐침잡효솔여신호여경전법차이무통계학의의(잡효솔99.57％ ±0.53％화99.70％ ±0.42％,t=1.30,P＞0.05;잡교신호2.22 ±0.42화2.36 ±0.48,t=1.57,P＞0.05);경전법여삼보법적형광원위잡교결과여전통배양결과부합솔위100％(36/36).결론 본연구중적개량삼보법조작간편,보취간단、실험효솔고、배보,단야존재18/X/Y신호편산적정황,유대우진일보모색.
Objective To investigate a simple and efficient fluorescence in situ hybridization method for prenatal diagnosis.Methods Thirty-six cases of chorionic villus samples were hybridized with probes 18/X/Y,13/21 by using traditional cuhure methods and modified three-step methods in controlled experimentation during 2012 to 2013.The hybridization rate and fluorescence signals were analyzed.Results A total of 72 hybrid zone were detected.Probe 21/13 hybridization rate and fluorescence signals of three-step modified methods were higher than that of traditional methods(99.72％ ±0.42％ vs 85.90％ ±4.15％,t =20.4,P ＜0.01; 2.58 ±0.50 vs 1.52 ± 0.55,t =7.53,P ＜0.01).Probe 18/X/Y has the same hybridization rate and fluorescence signals between three-step modified methods and traditional methods (99.57％ ±0.53％ vs99.70％ ±0.42％,t=1.30,P＞0.05; 2.22±0.42 vs2.36±0.48,t=1.57,P＞0.05).The coincidence rate of two methods was 100％ (36/36).Conclusion The modified fluorescence in situ hybridization three-step methods in the study was simple,rapid,effective and environment-friendly.At the same time,it has some defects such as the signals of 18/X/Y was not concentrated enough.Further exploration is needed.
