中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2014年
7期
531-534
,共4页
董敏%郑书发%余斐%陈晓%杨青%孔海深%俞小忠%黄龑%陈瑜
董敏%鄭書髮%餘斐%陳曉%楊青%孔海深%俞小忠%黃龑%陳瑜
동민%정서발%여비%진효%양청%공해심%유소충%황엄%진유
泌尿道%大肠杆菌%毒力%抗药性,细菌%基因型%流行病学,分子
泌尿道%大腸桿菌%毒力%抗藥性,細菌%基因型%流行病學,分子
비뇨도%대장간균%독력%항약성,세균%기인형%류행병학,분자
Urinary tract%Escherichia coli%Virulence%Drug resistance,bacterial%Genotype%Epidemiology,molecular
目的 了解杭州地区尿道致病性大肠埃希菌(UPEC)基因型的构成以及毒力和耐药性.方法 收集浙江大学医学院附属第一医院2011年7月至2012年6月从尿路感染患者尿液中分离的55株UPEC,对其开展系统分群和多位点序列分型,筛查46种毒力基因,采用纸片扩散法开展药敏试验.毒力分数组间比较采用秩和检验,率的组间比较采用卡方检验或Fisher检验.结果 55株UPEC中A、B1、B2和D群分别占9.1％、5.5％、43.6％和41.8％,毒力分数(毒力基因个数)的第50百分位数P50分别为6、7、14和9,A、B1和D群的毒力分数分别与B2群的比较,均有显著性差异(P分别为＜0.001、0.005和＜0.001).B2群中的ST73、ST95和ST493菌株(共9株,16.4％)的毒力分数高达15 ～ 20.11株(20.0％)ST131均属于B2群,是最常见的克隆群.有13种药物的耐药率ST131菌株大于非ST131,ST131菌株对环丙沙星耐药率高达10/11.结论 杭州地区UPEC中存在强毒力和(或)高耐药性的克隆株,必须高度重视,避免广泛流行.
目的 瞭解杭州地區尿道緻病性大腸埃希菌(UPEC)基因型的構成以及毒力和耐藥性.方法 收集浙江大學醫學院附屬第一醫院2011年7月至2012年6月從尿路感染患者尿液中分離的55株UPEC,對其開展繫統分群和多位點序列分型,篩查46種毒力基因,採用紙片擴散法開展藥敏試驗.毒力分數組間比較採用秩和檢驗,率的組間比較採用卡方檢驗或Fisher檢驗.結果 55株UPEC中A、B1、B2和D群分彆佔9.1％、5.5％、43.6％和41.8％,毒力分數(毒力基因箇數)的第50百分位數P50分彆為6、7、14和9,A、B1和D群的毒力分數分彆與B2群的比較,均有顯著性差異(P分彆為＜0.001、0.005和＜0.001).B2群中的ST73、ST95和ST493菌株(共9株,16.4％)的毒力分數高達15 ～ 20.11株(20.0％)ST131均屬于B2群,是最常見的剋隆群.有13種藥物的耐藥率ST131菌株大于非ST131,ST131菌株對環丙沙星耐藥率高達10/11.結論 杭州地區UPEC中存在彊毒力和(或)高耐藥性的剋隆株,必鬚高度重視,避免廣汎流行.
목적 료해항주지구뇨도치병성대장애희균(UPEC)기인형적구성이급독력화내약성.방법 수집절강대학의학원부속제일의원2011년7월지2012년6월종뇨로감염환자뇨액중분리적55주UPEC,대기개전계통분군화다위점서렬분형,사사46충독력기인,채용지편확산법개전약민시험.독력분수조간비교채용질화검험,솔적조간비교채용잡방검험혹Fisher검험.결과 55주UPEC중A、B1、B2화D군분별점9.1％、5.5％、43.6％화41.8％,독력분수(독력기인개수)적제50백분위수P50분별위6、7、14화9,A、B1화D군적독력분수분별여B2군적비교,균유현저성차이(P분별위＜0.001、0.005화＜0.001).B2군중적ST73、ST95화ST493균주(공9주,16.4％)적독력분수고체15 ～ 20.11주(20.0％)ST131균속우B2군,시최상견적극륭군.유13충약물적내약솔ST131균주대우비ST131,ST131균주대배병사성내약솔고체10/11.결론 항주지구UPEC중존재강독력화(혹)고내약성적극륭주,필수고도중시,피면엄범류행.
Objective To study the prevalence,virulence and antimicrobial resistance of uropathogenic Escherichia coli gene types in the area.Methods Fifty Escherichia coli clinical isolated from outpatients and inpatients with urinary tract infection in the First Affiliated Hospital,College of Medicine,Zhejiang University from July 2011 to June 2012 were chosen.All isolates were assessed for phylogenetic group,sequence type,46 virulence genes and antimicrobial resistance.Virulence scores were compared between groups by Rank-sum test,while the rates of groups were compared by Chi-square test or Fisher exact test.Results The 55 isolates were divided into phylogenetic group A,B1,B2 and D,accounting for 9.1％,5.5％,43.6％ and 41.8％,respectively.The virulence scores (number of virulence genes) P50 were 6,7,14 and 9,respectively.The virulence scores of group A,B1 and D were significantly different from that of group B2 (P ＜0.001,0.005 and ＜0.001 respectively).ST73,ST95 and ST493 isolates (9,16.4％) belonging to group B2,exhibited virulence genes up 15 to 20.B2-ST131 was the most common isolate,accounting for 20.0％ of the overall isolates.The ST131 isolates exhibited higher antimicrobial resistance to 13 kinds of antimicrobials than non-ST131 isolates.The resistance rate to ciprofloxacin of the ST131 isolates was 90.9％ (10/11).Conclusions It is important to pay attention to high prevalence of some UPEC clonal groups with enhanced virulence and(or) antimicrobial resistance in the area.And larger range of surveillance should be emphasized.
