国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2014年
19期
3003-3006
,共4页
丙肝肝炎病毒%抗丙肝病毒抗体%PCR-荧光探针法%化学发光微粒子免疫分析%酶联免疫吸附试验
丙肝肝炎病毒%抗丙肝病毒抗體%PCR-熒光探針法%化學髮光微粒子免疫分析%酶聯免疫吸附試驗
병간간염병독%항병간병독항체%PCR-형광탐침법%화학발광미입자면역분석%매련면역흡부시험
Hepatitis C Virus%Anti-HCV%PCR%CMIA%ELISA
目的 探讨三种检测方法作为临床筛选和诊断丙型肝炎的的优缺点.方法 随机收集71例丙肝患者血清作为阳性组,63例非丙肝患者血清作为阴性组,采用PCR-荧光探针法检测HCV RNA,同时采用CMIA法和ELISA法检测Anti-HCV.结果 阳性组中,PCR-荧光探针法灵敏度为87.32％,CMIA为100％,ELISA为97.18％;经卡方检验分析,x2=4.95,两种Anti-HCV检测方法均与PCR-荧光探针法检测结果的差异有统计学意义(P＜0.05);而两种Anti-HCV检测方法之间差异无统计学意义(P＞0.05);阴性组中,HCV RNA特异度为100％,CMIA为95.23％,ELISA为98.41％;经x2检验分析,x2=3.84,三种诊断方法特异度的差异无统计学意义(p＞0.05).结论 三种方法各有利弊,在诊断丙型肝炎方面,本文推荐采用其中一种抗体检测方法与PCR-荧光探针法联合检测;在疗效观察方面,本文推荐PCR-荧光探针法;在大规模体检时,考虑经济因素,本文推荐ELISA;在Anti-HCV检测结果为阳性,或者两种Anti-HCV检测方法结果不一致时,需联合HCV RNA检测.
目的 探討三種檢測方法作為臨床篩選和診斷丙型肝炎的的優缺點.方法 隨機收集71例丙肝患者血清作為暘性組,63例非丙肝患者血清作為陰性組,採用PCR-熒光探針法檢測HCV RNA,同時採用CMIA法和ELISA法檢測Anti-HCV.結果 暘性組中,PCR-熒光探針法靈敏度為87.32％,CMIA為100％,ELISA為97.18％;經卡方檢驗分析,x2=4.95,兩種Anti-HCV檢測方法均與PCR-熒光探針法檢測結果的差異有統計學意義(P＜0.05);而兩種Anti-HCV檢測方法之間差異無統計學意義(P＞0.05);陰性組中,HCV RNA特異度為100％,CMIA為95.23％,ELISA為98.41％;經x2檢驗分析,x2=3.84,三種診斷方法特異度的差異無統計學意義(p＞0.05).結論 三種方法各有利弊,在診斷丙型肝炎方麵,本文推薦採用其中一種抗體檢測方法與PCR-熒光探針法聯閤檢測;在療效觀察方麵,本文推薦PCR-熒光探針法;在大規模體檢時,攷慮經濟因素,本文推薦ELISA;在Anti-HCV檢測結果為暘性,或者兩種Anti-HCV檢測方法結果不一緻時,需聯閤HCV RNA檢測.
목적 탐토삼충검측방법작위림상사선화진단병형간염적적우결점.방법 수궤수집71례병간환자혈청작위양성조,63례비병간환자혈청작위음성조,채용PCR-형광탐침법검측HCV RNA,동시채용CMIA법화ELISA법검측Anti-HCV.결과 양성조중,PCR-형광탐침법령민도위87.32％,CMIA위100％,ELISA위97.18％;경잡방검험분석,x2=4.95,량충Anti-HCV검측방법균여PCR-형광탐침법검측결과적차이유통계학의의(P＜0.05);이량충Anti-HCV검측방법지간차이무통계학의의(P＞0.05);음성조중,HCV RNA특이도위100％,CMIA위95.23％,ELISA위98.41％;경x2검험분석,x2=3.84,삼충진단방법특이도적차이무통계학의의(p＞0.05).결론 삼충방법각유리폐,재진단병형간염방면,본문추천채용기중일충항체검측방법여PCR-형광탐침법연합검측;재료효관찰방면,본문추천PCR-형광탐침법;재대규모체검시,고필경제인소,본문추천ELISA;재Anti-HCV검측결과위양성,혹자량충Anti-HCV검측방법결과불일치시,수연합HCV RNA검측.
Objective To explore the advantages and disadvantages of three methods for clinical screening and diagnosis in Hepatitis C.Methods 71 cases of Hepatitis C patients for experimental group,while 63 negative samples excluding Hepatitis C as control group.Antibody Hepatitis C Virus (Anti-HCV) was detected by PCR-fluorescent probe,chemiluminesent microparticle immunoassay(CMIA) and enzyme linked immunosorbent assay(ELISA) respectively.Results In the positive group,the sensitivity of PCR-fluorescent probe was 87.32％,CMIA was 100％,ELISA was 97.18％; Analyzed by chi-square test,x2=4.95,the difference between PCR-fluorescent probe and other kinds of methods was statistically significant (P ＜0.05),while no significant difference between CMIA and ELISA (P＞ 0.05).In control group,the specificity of PCR-fluorescent probe was 100％,CMIA 95.23％,ELISA 98.41％,respectively; Analyzed by x2test,the specificity of three methods had no statistically significant (P＞ 0.05),x2=3.84.Conclusions There were advantages and disadvantages for three methods in the diagnosis of Hepatitis C.One of the methods in CMIA and ELISA combined with PCR-fluorescence probe was recommended for diagnosing Hepatitis C.If you are going to monitor the treatment effect,PCR-fluorescence probe must be used; However,ELISA is widely used in most of the hospitals for the economic reason.When the result of Anti-HCV was suspicious or positive,PCR was needed to be detected to conform its authenticity.
