CAJ | 학술논문

目的观察应用微等离子束对皮肤组织的结构变化及其超微结构变化的作用效应,初步探讨微等离子束的作用机制.方法选择30只豚鼠,随机分为3组,分别给予40 W、10 kJ,60 W、10 kJ和80 W、10 kJ的微等离子束照射,每只豚鼠背部划分为实验侧和空白对照侧2个等分区域,于作用后即刻、1周后和1个月后分别切取实验侧及空白对照侧皮肤行组织病理和透射电镜检测.结果不同剂量设置作用于豚鼠皮肤即刻变化明显不同,40 W、10 kJ作用时,表皮基本完整,真皮浅层胶原组织可见轻微均质化；60 W、10 kJ作用时,表皮局灶性出现点阵状改变,真皮浅层胶原组织出现点阵化和明显均质化；80 W、10 kJ作用时,表皮完全汽化缺失或者坏死变性,真皮浅中层胶原组织出现大面积均质化.1周后皮肤浅层胶原组织结构逐渐致密,排列有序,1个月后皮肤浅层胶原组织明显增厚,排列致密.透射电镜显示,作用后表皮细胞较完整,细胞间结构正常,但真皮胶原丧失正常结构,细胞结构消失,大量细胞凋亡明显,1个月后仍可见少量细胞凋亡,但胶原结构逐渐恢复.结论微等离子束对皮肤组织作用有明显的剂量效应,其主要靶组织为真皮胶原组织,可以明显刺激皮肤新生胶原的增生.
목적 관찰응용미등리자속대피부조직적결구변화급기초미결구변화적작용효응,초보탐토미등리자속적작용궤제.방법 선택30지돈서,수궤분위3조,분별급여40 W、10 kJ,60 W、10 kJ화80 W、10 kJ적미등리자속조사,매지돈서배부화분위실험측화공백대조측2개등분구역,우작용후즉각、1주후화1개월후분별절취실험측급공백대조측피부행조직병리화투사전경검측.결과 불동제량설치작용우돈서피부즉각변화명현불동,40 W、10 kJ작용시,표피기본완정,진피천층효원조직가견경미균질화；60 W、10 kJ작용시,표피국조성출현점진상개변,진피천층효원조직출현점진화화명현균질화；80 W、10 kJ작용시,표피완전기화결실혹자배사변성,진피천중층효원조직출현대면적균질화.1주후피부천층효원조직결구축점치밀,배렬유서,1개월후피부천층효원조직명현증후,배렬치밀.투사전경현시,작용후표피세포교완정,세포간결구정상,단진피효원상실정상결구,세포결구소실,대량세포조망명현,1개월후잉가견소량세포조망,단효원결구축점회복.결론 미등리자속대피부조직작용유명현적제량효응,기주요파조직위진피효원조직,가이명현자격피부신생효원적증생.
Objective To investigate the structural and ultrastructural changes of the skin induced by micro-plasma radio-frequency technology,and to preliminarily discuss this novel technology mechanism.Methods Thirty guinea pigs were enrolled and randomly divided into three groups.They were radiated by different dose parameters 40 W,10 kJ; 60 W,10 kJ and 80 W,10 kJ.Every guinea pig's back was divided into two parts which was removed after immediately,one week and one month,and dermatopathology and transmission electron microscopy (TEM) were performed.Results The different dose setting could make different skin change of immediately effect.When dose setting was 40 W,10 kJ,skin showed that epidermal cells were integrity and the superficial layer of dermis collagen tissue was light homogenization.When dose setting was 60 W,10 kJ,epidermal tissue showed focal emergence of fractional shape change and obvious homogenization.When dose setting was 80 W,10 kJ,epidermal showed complete vaporization loss or degeneration necrosis,and dermal superficial and middle layer of collagen tissue showed a large area of homogenization.Skin superficial collagen tissue's structure gradually showed dense and arranged in an orderly manner after one week and markedly thickened and arranged in compact manner after one month.TEM showed that epidermal cells were relatively complete,intercellular structure was normal,but the dermal collagen lost originally normal structure and cell structure disappeared and obviously showed massive apoptosis.A small amount of apoptosis was showed but collagen structure gradually restored after one month.Conclusions The novel micro-plasma radio-frequency has obvious dose effect to skin,and its main target tissues are dermal collagen tissue.It can stimulate skin collagen hyperplasia in certain degree.