CAJ | 학술논문

目的探讨自体富血小板纤维蛋白(platelet-rich fibrin,PRF)对体外培养人脂肪来源干细胞(adipose-derived stem cells,ADSCs)增殖及成脂分化的影响.方法将自愿捐献由脂肪抽吸术获取的脂肪组织进行分离培养ADSCs并鉴定.将第3代ADSCs分为空白对照组和1个PRF膜片组(1PRFM组)和2个PRF膜片组(2PRFM组).倒置显微镜观察细胞生长情况,培养后1、2、3、4、5、6、7d采用四甲基偶氮噻唑蓝比色法(MTT)法检测细胞增殖活性.在第3、5、7、9、11和14天时采用油红O染色法检测细胞成脂分化情况.结果随着PRFM剂量的增加,细胞增殖数量和成脂率增加,3组差异具有显著统计学意义.结论 PRF能明显促进ADSCs增殖和成脂分化,可以作为自体材料应用于脂肪组织工程的研究.
목적 탐토자체부혈소판섬유단백(platelet-rich fibrin,PRF)대체외배양인지방래원간세포(adipose-derived stem cells,ADSCs)증식급성지분화적영향.방법 장자원연헌유지방추흡술획취적지방조직진행분리배양ADSCs병감정.장제3대ADSCs분위공백대조조화1개PRF막편조(1PRFM조)화2개PRF막편조(2PRFM조).도치현미경관찰세포생장정황,배양후1、2、3、4、5、6、7d채용사갑기우담새서람비색법(MTT)법검측세포증식활성.재제3、5、7、9、11화14천시채용유홍O염색법검측세포성지분화정황.결과 수착PRFM제량적증가,세포증식수량화성지솔증가,3조차이구유현저통계학의의.결론 PRF능명현촉진ADSCs증식화성지분화,가이작위자체재료응용우지방조직공정적연구.
Objective To study the effect of autogeneic platelet-rich fibrin (PRF) on proliferation and adipogenic differentiation of human adipose-derived stem cells (ADSCs) in vitro.Methods ADSCs were isolated from adipose tissue obtained from donors undergoing liposuction and were cultured,and underwent identification.ADSCs at passage 3 were divided into three groups:test groups were cultured with 1PRFM and 2PRFM,and control group was cultured without PRF membrane.Then the growth of the cells was observed by inverted microscope.MTT method was used to observe cell proliferation activity at days 1,2,3,4,5,6 and 7 after culture.Adipogenic differentiation of ADSCs was observed and quantified by oil red O staining at days 3,5,7,9,11 and 14.Results Cell proliferation and adipogenic differentiation would be increased with the PRFM,There were significant differences among three groups.Conclusions PRF could significantly promote proliferation and adipogenic differentiation of ADSCs.