CAJ | 학술논문

目的探讨瘢痕疙瘩成纤维细胞中核心蛋白多糖的表达、含量,及其在瘢痕疙瘩形成中的作用和机制.方法对瘢痕疙瘩、正常瘢痕以及正常皮肤成纤维细胞进行体外培养,采用光镜、透射电镜观察成纤维细胞形态、活性及凋亡；应用实时荧光定量聚合酶链式反应(FQ-PCR)对核心蛋白多糖以及β1转化生长因子(TGF-β1)的mRNA表达进行检测、分析.结果瘢痕疙瘩成纤维细胞形态不规则、排列紊乱,线粒体增多,粗面内质网扩张呈囊,细胞核常染色质丰富,表明其合成蛋白的功能活跃；瘢痕疙瘩成纤维细胞中核心蛋白多糖mRNA含量较正常瘢痕或正常皮肤成纤维细胞降低,而TGF-β1 mRNA表达则较正常皮肤及瘢痕组织成纤维细胞升高.结论核心蛋白多糖在瘢痕疙瘩成纤维细胞内含量较正常皮肤明显减少,提示其对成纤维细胞增殖、合成的抑制作用随之减弱,同时使TGF-β1表达上调,导致成纤维细胞的大量增生、迁移,合成过量胶原.表明核心蛋白多糖是抑制瘢痕疙瘩形成的重要因子.
목적 탐토반흔흘탑성섬유세포중핵심단백다당적표체、함량,급기재반흔흘탑형성중적작용화궤제.방법대반흔흘탑、정상반흔이급정상피부성섬유세포진행체외배양,채용광경、투사전경관찰성섬유세포형태、활성급조망；응용실시형광정량취합매련식반응(FQ-PCR)대핵심단백다당이급β1전화생장인자(TGF-β1)적mRNA표체진행검측、분석.결과 반흔흘탑성섬유세포형태불규칙、배렬문란,선립체증다,조면내질망확장정낭,세포핵상염색질봉부,표명기합성단백적공능활약；반흔흘탑성섬유세포중핵심단백다당mRNA함량교정상반흔혹정상피부성섬유세포강저,이TGF-β1 mRNA표체칙교정상피부급반흔조직성섬유세포승고.결론 핵심단백다당재반흔흘탑성섬유세포내함량교정상피부명현감소,제시기대성섬유세포증식、합성적억제작용수지감약,동시사TGF-β1표체상조,도치성섬유세포적대량증생、천이,합성과량효원.표명핵심단백다당시억제반흔흘탑형성적중요인자.
Objective To detect the expression and content of decorin in fibroblasts of keloid to deeply reveal the mechenism and the role of decorin plays in scar formation.Methods Fibroblasts of keloid,normal scar and normal skin were cultured in vitro,and the morphology,activity,apoptosis of fibroblast were observed under light microscope and electron microscope; the mRNAs of decorin and TGF-β1 were detected and analyzed with real-time fluorescent quantitative-PCR (FQ-PCR).Results Fibroblasts of keloid showed irregular morphology,larger size and disorder arrangement.There were a large number of mitochondria,swelling rough endoplasmic reticulum,and euchromatin-rich in nucleus of fibroblasts,suggesting the protein synthesis of keloid fibroblast was very active.Compared with normal skin,the expression of decorin was significantly lower in keloid fibroblast; On the contrary,the expression of TGF-β1 was significantly higher in keloid fibroblast than in normal scar and normal skin.Conclusions Compared with normal skin,the expression of decorin in keloid fibroblast is significantly lower.Lower content of decorin in early stage of wound healing may induce weakly suppression of proliferation and synthesis of fibroblast,and up-regulate the activity of TGF-β1,which promotes the proliferation,migration and excessive collagen synthesis of the fibroblast of keloid.Thus,decorinis an suppressor factor of keloid formation.