中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2014年
1期
62-65
,共4页
赵雪莲%苏晓光%张锤%张春莉%张卓男%郑宝恒
趙雪蓮%囌曉光%張錘%張春莉%張卓男%鄭寶恆
조설련%소효광%장추%장춘리%장탁남%정보항
增生性瘢痕%瘢痕皮回植术%病理性瘢痕%基质金属蛋白酶-1%金属蛋白酶组织抑制剂-1
增生性瘢痕%瘢痕皮迴植術%病理性瘢痕%基質金屬蛋白酶-1%金屬蛋白酶組織抑製劑-1
증생성반흔%반흔피회식술%병이성반흔%기질금속단백매-1%금속단백매조직억제제-1
Hypertrophic scar%Scar skin replantation%Pathological scar%Matrix metalloproteinase-1 (MMP-1)%Tissue inhibitor of metalloproteinase-1 (TIMP-1)
目的 通过建立兔耳模型观察基质金属蛋白酶-1 (matrix metalloproteinase-1,MMP-1)及金属蛋白酶组织抑制剂-1(tissue inhibitor of metalloproteinase-1,TIMP-1)在病理性瘢痕皮回植术后组织中表达的变化,探讨瘢痕皮回植治疗病理性瘢痕的机制.方法 建立兔耳病理性瘢痕模型,共分为3组:正常皮肤组(对照组,A组)、病理性瘢痕组(B组)及瘢痕皮回植组(C组).切取标本行HE染色和Masson特殊组织化学染色及免疫组织化学染色,观察各组标本MMP-1、TIMP-1的表达情况.结果 病理性瘢痕经瘢痕皮回植术后,MMP-1及TIMP-1均较A组明显升高(P＜0.01),MMP-1的表达较TIMP-1明显增强(P＜0.01).结论 瘢痕皮回植术治疗瘢痕的机制与瘢痕组织内MMP-1和TIMP-1相互作用的失衡有关.
目的 通過建立兔耳模型觀察基質金屬蛋白酶-1 (matrix metalloproteinase-1,MMP-1)及金屬蛋白酶組織抑製劑-1(tissue inhibitor of metalloproteinase-1,TIMP-1)在病理性瘢痕皮迴植術後組織中錶達的變化,探討瘢痕皮迴植治療病理性瘢痕的機製.方法 建立兔耳病理性瘢痕模型,共分為3組:正常皮膚組(對照組,A組)、病理性瘢痕組(B組)及瘢痕皮迴植組(C組).切取標本行HE染色和Masson特殊組織化學染色及免疫組織化學染色,觀察各組標本MMP-1、TIMP-1的錶達情況.結果 病理性瘢痕經瘢痕皮迴植術後,MMP-1及TIMP-1均較A組明顯升高(P＜0.01),MMP-1的錶達較TIMP-1明顯增彊(P＜0.01).結論 瘢痕皮迴植術治療瘢痕的機製與瘢痕組織內MMP-1和TIMP-1相互作用的失衡有關.
목적 통과건립토이모형관찰기질금속단백매-1 (matrix metalloproteinase-1,MMP-1)급금속단백매조직억제제-1(tissue inhibitor of metalloproteinase-1,TIMP-1)재병이성반흔피회식술후조직중표체적변화,탐토반흔피회식치료병이성반흔적궤제.방법 건립토이병이성반흔모형,공분위3조:정상피부조(대조조,A조)、병이성반흔조(B조)급반흔피회식조(C조).절취표본행HE염색화Masson특수조직화학염색급면역조직화학염색,관찰각조표본MMP-1、TIMP-1적표체정황.결과 병이성반흔경반흔피회식술후,MMP-1급TIMP-1균교A조명현승고(P＜0.01),MMP-1적표체교TIMP-1명현증강(P＜0.01).결론 반흔피회식술치료반흔적궤제여반흔조직내MMP-1화TIMP-1상호작용적실형유관.
Objective To observe the changes of MMP-1 and TIMP-1 expression in the pathological scar tissue after scar-skin replantation and to explore the mechanism of treating pathological scars with scar-skin replantation through a rabbit ear model.Methods Rabbit ears were used to establish the hypertrophic scar animal model in this study.Specimens were taken for three times:normal skin,hypertrophic scar and scar-skin replantion separately.We then performed HE staining,Masson staining and immunohistochemical staining to observe the expression of MMP-1 and TIMP-1 in these three groups of specimens.Results The expression of both MMP-1 and TIMP-1 significantly increased in the hypertrophic scar tissue after scar-skin replantation compared with the control group (P＜0.01).The expression of MMP-1 increased more significantly than that of TIMP-1 (P＜0.01).Conclusions The mechanism of scar-skin replantation's effect in the treatment for hypertrophic scar is relevant to the imbalance in the interaction between MMP-1 and TIMP-1 in the scar tissue.
