中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2014年
2期
134-138
,共5页
赵彩玲%匡瑞霞%刘肃%闫丙健
趙綵玲%劻瑞霞%劉肅%閆丙健
조채령%광서하%류숙%염병건
二甲氨基乙醇%超氧化物歧化酶%羟脯氨酸%丙二醛
二甲氨基乙醇%超氧化物歧化酶%羥脯氨痠%丙二醛
이갑안기을순%초양화물기화매%간포안산%병이철
Dimethylaminoethanol (DMAE)%Superoxide dismutase (SOD)%Hydroxyproline (HYP)%Malondialdehyde (MDA)
目的 探讨真皮内注射二甲氨基乙醇(DMAE)和复方氨基酸溶液(AA)对皮肤胶原代谢和自由基的影响.方法 80只大鼠随机分成衰老治疗组(60只)、衰老对照组(10只)和正常对照组(10只).皮下注射半乳糖制备衰老模型.衰老治疗组从第18天开始,在两侧臀部真皮内分别注射0.2％ DMAE+AA、0.1％ DMAE+AA、0.2％ DMAE、0.1％ DMAE、AA、生理盐水(NS),每周1次,连续4周.观察各组皮肤苏木精-伊红染色法(HE)切片组织学变化并测定皮肤中含水量、超氧化物歧化酶(SOD)活力、羟脯氨酸(HYP)和丙二醛(MDA)含量.结果 0.2％DMAE+ AA组、0.1％ DMAE+AA组表皮、真皮厚度和胶原纤维面积均高于衰老对照组,差异有统计学意义(P＜0.05).0.2％ DMAE+AA组、0.1％ DMAE+ AA组与其他各衰老组相比,HYP含量增加、SOD活力升高、MDA含量降低,其组间差异具有统计学意义(P＜0.05).正常对照组、0.2％ DMAE+AA组、0.1％DMAE+ AA组之间HYP含量差异无统计学意义(P＞0.05).各组大鼠皮肤含水量差异无统计学意义(P＜0.05).结论 真皮内注射0.2％ DMAE+AA、0.1％ DMAE+AA能显著增加表皮、真皮厚度和胶原纤维面积及HYP含量,提高SOD活力、降低MDA含量,表明其有清除皮肤自由基、提高抗氧化能力和促进皮肤胶原代谢的作用,从而延缓皮肤衰老.
目的 探討真皮內註射二甲氨基乙醇(DMAE)和複方氨基痠溶液(AA)對皮膚膠原代謝和自由基的影響.方法 80隻大鼠隨機分成衰老治療組(60隻)、衰老對照組(10隻)和正常對照組(10隻).皮下註射半乳糖製備衰老模型.衰老治療組從第18天開始,在兩側臀部真皮內分彆註射0.2％ DMAE+AA、0.1％ DMAE+AA、0.2％ DMAE、0.1％ DMAE、AA、生理鹽水(NS),每週1次,連續4週.觀察各組皮膚囌木精-伊紅染色法(HE)切片組織學變化併測定皮膚中含水量、超氧化物歧化酶(SOD)活力、羥脯氨痠(HYP)和丙二醛(MDA)含量.結果 0.2％DMAE+ AA組、0.1％ DMAE+AA組錶皮、真皮厚度和膠原纖維麵積均高于衰老對照組,差異有統計學意義(P＜0.05).0.2％ DMAE+AA組、0.1％ DMAE+ AA組與其他各衰老組相比,HYP含量增加、SOD活力升高、MDA含量降低,其組間差異具有統計學意義(P＜0.05).正常對照組、0.2％ DMAE+AA組、0.1％DMAE+ AA組之間HYP含量差異無統計學意義(P＞0.05).各組大鼠皮膚含水量差異無統計學意義(P＜0.05).結論 真皮內註射0.2％ DMAE+AA、0.1％ DMAE+AA能顯著增加錶皮、真皮厚度和膠原纖維麵積及HYP含量,提高SOD活力、降低MDA含量,錶明其有清除皮膚自由基、提高抗氧化能力和促進皮膚膠原代謝的作用,從而延緩皮膚衰老.
목적 탐토진피내주사이갑안기을순(DMAE)화복방안기산용액(AA)대피부효원대사화자유기적영향.방법 80지대서수궤분성쇠로치료조(60지)、쇠로대조조(10지)화정상대조조(10지).피하주사반유당제비쇠로모형.쇠로치료조종제18천개시,재량측둔부진피내분별주사0.2％ DMAE+AA、0.1％ DMAE+AA、0.2％ DMAE、0.1％ DMAE、AA、생리염수(NS),매주1차,련속4주.관찰각조피부소목정-이홍염색법(HE)절편조직학변화병측정피부중함수량、초양화물기화매(SOD)활력、간포안산(HYP)화병이철(MDA)함량.결과 0.2％DMAE+ AA조、0.1％ DMAE+AA조표피、진피후도화효원섬유면적균고우쇠로대조조,차이유통계학의의(P＜0.05).0.2％ DMAE+AA조、0.1％ DMAE+ AA조여기타각쇠로조상비,HYP함량증가、SOD활력승고、MDA함량강저,기조간차이구유통계학의의(P＜0.05).정상대조조、0.2％ DMAE+AA조、0.1％DMAE+ AA조지간HYP함량차이무통계학의의(P＞0.05).각조대서피부함수량차이무통계학의의(P＜0.05).결론 진피내주사0.2％ DMAE+AA、0.1％ DMAE+AA능현저증가표피、진피후도화효원섬유면적급HYP함량,제고SOD활력、강저MDA함량,표명기유청제피부자유기、제고항양화능력화촉진피부효원대사적작용,종이연완피부쇠로.
Objective To study the mechanism of anti-aging effect of dimethylaminoethanol (DMAE) and compound amino acid (AA) injection by mesotheray in D-galactose-induced skin aging rat.Methods Eighty rats were randomly divided into aging treatment group (60 cases),aging control group (10 cases) and normal control group (10 cases).The skin aging models were established by subcutaneous infectim of D-galactose.From the 18th day,the aging treatment group were injected intradermally in the rats' both sides hip skin with 0.2％ DMAE+ AA,0.1％ DMAE+AA,0.2％DMAE,0.1％ DMAE,AA,and saline,once a week.After four weeks,HE,water content,superoxide dismutase (SOD) activity,hydroxyproline (HYP) and malondialdehyde (MDA) content were measured.Results Compared with the aging control group,the epidermal and the dermal thickness and the collagen area of rats skin' increased significantly in 0.2 ％ DMAE+ AA and 0.1％ DMAE+ AA treatment groups (P＜0.05).0.2％ DMAE+AA and 0.1 ％ DMAE+AA treatment groups also had higher SOD activity,HYP content and lower MDA content than other groups (P＜0.05),but no difference was noted among normal control group,0.2％ DMAE+AA and 0.1％ DMAE+AA group (P＞0.05).There were no differences in water content among groups (P＞0.05).Conclusions Intradermal injection with 0.1％ DMAE+ AA and 0.2 ％ DMAE+AA in aging rats may increase the epidermal and the dermal thickness and the collagen of rats skin' improve SOD activity,HYP content and decreased MDA content,indicating that it has ability to clear skin free radicals,enhance antioxidant capacity and skin collagen metabolism,and thus prevent skin aging.
