CAJ | 학술논문

目的探讨表皮生长因子(epidermal growth factor,EGF)对脂肪干细胞(adipose-derived stem cells,ADSCs)旁分泌促血管生成因子及促进人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)小管形成的影响.方法体外原代培养ADSCs,流式细胞术分析细胞表面标志物表达,酶联免疫吸附法(ELISA)检测不同浓度EGF刺激前后ADSCs-CM中血管内皮生长因子(vascular endothelial growth factor,VEGF)、肝细胞生长因子(hepatoeyte growth factor,HGF)、间质源性因子-1 (stromal-cell derived factor-1,SDF-1)含量变化;HUVEC小管形成实验检测EGF预处理对ADSCs促进HUVEC形成管样毛细血管的影响.结果利用脂肪抽吸液成功培养出ADSCs,ADSCs具有其特异的表面标记物表达;ADSCs可分泌各种促血管生成因子,且EGF刺激可增加其分泌量;ADSCs可促进共培养的HUVEC形成管样毛细血管,用EGF预处理ADSCs后该作用显著提高.结论体外环境下ADSCs可分泌多种促血管生成因子,并可促进共培养的HUVEC形成管样毛细血管;EGF刺激可增强该作用,15 mg/L EGF即可达到最佳效果.
목적 탐토표피생장인자(epidermal growth factor,EGF)대지방간세포(adipose-derived stem cells,ADSCs)방분비촉혈관생성인자급촉진인제정맥내피세포(human umbilical vein endothelial cells,HUVEC)소관형성적영향.방법 체외원대배양ADSCs,류식세포술분석세포표면표지물표체,매련면역흡부법(ELISA)검측불동농도EGF자격전후ADSCs-CM중혈관내피생장인자(vascular endothelial growth factor,VEGF)、간세포생장인자(hepatoeyte growth factor,HGF)、간질원성인자-1 (stromal-cell derived factor-1,SDF-1)함량변화;HUVEC소관형성실험검측EGF예처리대ADSCs촉진HUVEC형성관양모세혈관적영향.결과 이용지방추흡액성공배양출ADSCs,ADSCs구유기특이적표면표기물표체;ADSCs가분비각충촉혈관생성인자,차EGF자격가증가기분비량;ADSCs가촉진공배양적HUVEC형성관양모세혈관,용EGF예처리ADSCs후해작용현저제고.결론 체외배경하ADSCs가분비다충촉혈관생성인자,병가촉진공배양적HUVEC형성관양모세혈관;EGF자격가증강해작용,15 mg/L EGF즉가체도최가효과.
Objective To explore the effect of epidermal growth factor (EGF) on tube formation of HUVEC induced by the secretion of angiogenesis factors of adipose-derived stem cells (ADSCs).Methods ADSCs were primarily cultured by enzyme digestion method.The flow cytomertry was performed to detect the expression of cell surface marker.ELISA was used to detect the expression of VEGF,HGF,and SDF-1 after given different doses of EGF.Tube formation assay was used to examine the effect of EGF on the tube formation induced by ADSCs.Results ADSCs were successfully isolated and cultured from human liposuction tissue and specific markers were expressed on ADSCs.EGF promoted the secretion of angiogenesis factors VEGF,HGF,and SDF-1,which were secreted by ADSCs.EGF pretreatment increased the ability of tube formation of HUVECs induced by ADSCs.Conclusions ADSCs induce the secretion of angiogenesis factors in vitro,and thus increase the ability of tube formation of HUVECs.EGF promotes the secretion ability of ADSCs,and the best concentration is 15 mg/L.