中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2014年
3期
215-218
,共4页
兔耳瘢痕%电子线%曲安奈德%成纤维细胞
兔耳瘢痕%電子線%麯安奈德%成纖維細胞
토이반흔%전자선%곡안내덕%성섬유세포
Rabbit ear scar%Electron beam%Triamcinolone acetonide%Fibroblast
目的 观察采用生理盐水、曲安奈德、手术、电子线照射等治疗对兔耳增生性瘢痕的形态学影响,探寻治疗瘢痕的最优方案.方法 建立兔耳腹侧增生性瘢痕模型.随机分为空白组(A组)、生理盐水组(B组)、手术加生理盐水组(C组)、曲安奈德组(D组)、手术加曲安奈德组(E组)、电子线组(F组)、手术后2d电子线组(G组)、手术后7d电子线组(H组),通过HE染色、VG染色、甲苯胺蓝染色、CD34和α-SMA免疫组织化学行组织形态学观察.结果 胶原纤维观察显示:A、B组,D、G组,F、H组差异无统计学意义(P＞0.05),其余各组两两比较差异有统计学意义(P＜0.05).甲苯胺蓝染色发现肥大细胞不典型.微血管观察显示:A、B组,F、H组,G、H组差异无统计学意义(P＞0.05),其余各组两两比较差异有统计学意义(P＜0.05).成肌纤维细胞观察显示:A、B组差异无统计学意义(P＞0.05),其余各组两两比较差异有统计学意义(P＜0.05).结论 曲安奈德注射治疗兔耳瘢痕效果优于电子线放疗,电子线术后早期放疗效果优于愈合后放疗,曲安奈德、电子线联合手术治疗效果优于单一治疗.
目的 觀察採用生理鹽水、麯安奈德、手術、電子線照射等治療對兔耳增生性瘢痕的形態學影響,探尋治療瘢痕的最優方案.方法 建立兔耳腹側增生性瘢痕模型.隨機分為空白組(A組)、生理鹽水組(B組)、手術加生理鹽水組(C組)、麯安奈德組(D組)、手術加麯安奈德組(E組)、電子線組(F組)、手術後2d電子線組(G組)、手術後7d電子線組(H組),通過HE染色、VG染色、甲苯胺藍染色、CD34和α-SMA免疫組織化學行組織形態學觀察.結果 膠原纖維觀察顯示:A、B組,D、G組,F、H組差異無統計學意義(P＞0.05),其餘各組兩兩比較差異有統計學意義(P＜0.05).甲苯胺藍染色髮現肥大細胞不典型.微血管觀察顯示:A、B組,F、H組,G、H組差異無統計學意義(P＞0.05),其餘各組兩兩比較差異有統計學意義(P＜0.05).成肌纖維細胞觀察顯示:A、B組差異無統計學意義(P＞0.05),其餘各組兩兩比較差異有統計學意義(P＜0.05).結論 麯安奈德註射治療兔耳瘢痕效果優于電子線放療,電子線術後早期放療效果優于愈閤後放療,麯安奈德、電子線聯閤手術治療效果優于單一治療.
목적 관찰채용생리염수、곡안내덕、수술、전자선조사등치료대토이증생성반흔적형태학영향,탐심치료반흔적최우방안.방법 건립토이복측증생성반흔모형.수궤분위공백조(A조)、생리염수조(B조)、수술가생리염수조(C조)、곡안내덕조(D조)、수술가곡안내덕조(E조)、전자선조(F조)、수술후2d전자선조(G조)、수술후7d전자선조(H조),통과HE염색、VG염색、갑분알람염색、CD34화α-SMA면역조직화학행조직형태학관찰.결과 효원섬유관찰현시:A、B조,D、G조,F、H조차이무통계학의의(P＞0.05),기여각조량량비교차이유통계학의의(P＜0.05).갑분알람염색발현비대세포불전형.미혈관관찰현시:A、B조,F、H조,G、H조차이무통계학의의(P＞0.05),기여각조량량비교차이유통계학의의(P＜0.05).성기섬유세포관찰현시:A、B조차이무통계학의의(P＞0.05),기여각조량량비교차이유통계학의의(P＜0.05).결론 곡안내덕주사치료토이반흔효과우우전자선방료,전자선술후조기방료효과우우유합후방료,곡안내덕、전자선연합수술치료효과우우단일치료.
Objective To observe the morphological changes by different electron beam radiation methods and different triamcinolone acetonide treatments on the role of hypertrophic scar of rabbit ear,and to explore the optimal treatment for the rabbit ear scar.Methods The ventral rabbit ear hypertrophic scar model was established,and intervention began at 28 days after surgery.The animals were randomly divided into control group (group A),saline group (group B),surgery plus saline group (group C),triamcinolone acetonide group (group D),surgery plus triamcinolone group (group E),and electron beam group (group F),2 days after surgical electron beam group (group G),and 7 days after surgical electron beam group (group H).HE stain,VG stain,toluidine blue stain,CD34 and Alpha-SMA immunohistochemical staining were performed,and comparied on histomorphology of each group.Results Collagen fibers observed showed that there was no statistically significance between groups A and B,groups D and G and groups F and H (P＞0.05),and the difference was statistically significant between other groups (P＜0.05).Mast cells were not typical by toluidine blue staining.The microvascular observation showed there was no statistically significance between groups A and B,groups F and H,and groups G and H (P＞0.05),and the difference was statistically significant between other groups (P＜0.05).Myofibroblasts showed no statistically significance between the groups A and B (P＞0.05),but the difference was statistically significant between other groups (P＜0.05).Conclusions Compared with electron beam radiation methods,triamcinolone acetonide injection is a better treatment.Early postoperative radiotherapy is better than late.Triamcinolone acetonide or electron beam radiation combined with surgery is better than simple treatment.
