CAJ | 학술논문

目的观察光暴露及非光暴露部位的皮肤组织中晚期糖基化终末产物(AGEs)的表达特点,同时对比观察弹性纤维的形态改变,探讨非酶糖基化反应(non-enzymatic glycation,NEG)与光老化皮肤形态学改变的关系.方法对30例不同年龄的光暴露和非光暴露皮肤标本,采用醛品红法染色观察弹性纤维的变化及SP免疫组织化学检测AGEs表达.结果光暴露部位弹力纤维变性分级++以上标本中,均有明显的AGEs表达;++以下标本有线状分布的AGEs表达.非光暴露标本中,AGEs表达均为阴性.同一年龄,光暴露和非光暴露标本中,光暴露部位的AGEs的表达明显高于非光暴露部位,同时出现弹性纤维明显增多、增粗,出现扭曲、不规则分布.AGEs的表达同弹性纤维发生变性部位基本相符.结论 NEG反应在皮肤光老化的发生机制中可能有一定的作用.
목적 관찰광폭로급비광폭로부위적피부조직중만기당기화종말산물(AGEs)적표체특점,동시대비관찰탄성섬유적형태개변,탐토비매당기화반응(non-enzymatic glycation,NEG)여광노화피부형태학개변적관계.방법 대30례불동년령적광폭로화비광폭로피부표본,채용철품홍법염색관찰탄성섬유적변화급SP면역조직화학검측AGEs표체.결과 광폭로부위탄력섬유변성분급++이상표본중,균유명현적AGEs표체;++이하표본유선상분포적AGEs표체.비광폭로표본중,AGEs표체균위음성.동일년령,광폭로화비광폭로표본중,광폭로부위적AGEs적표체명현고우비광폭로부위,동시출현탄성섬유명현증다、증조,출현뉴곡、불규칙분포.AGEs적표체동탄성섬유발생변성부위기본상부.결론 NEG반응재피부광노화적발생궤제중가능유일정적작용.
Objective To detect the expression of advanced glycation end-products (AGEs) in the sun exposure and non-exposure skin,to observe the elastic protein in the same location,and to explore the relationship between non-enzymatic glycation reactions and,photo-aging skin morphological changes.Methods In the exposure and non-exposure specimens from 30 patients,elastic fibers were stained with Gomori staining,and immunohistochemistry for AGEs was performed.Results AGEs expressed clearly positive in all elastic fibers of degeneration markedly (+ + above) of exposure skin,and appeared line-like in markedly(+ + above)elastic fibers.While in all non-exposure skin,AGEs expressed negative.In the exposure skin with middle or old age,compared with non-exposure skin,AGEs expressed more and elastic fibers were hyperplasia with thickening,curling and irregular distribution.The skin parts of AGEs expressed accorded with where elastic fibers degenerated.Conclusions Ultraviolet radiation can induce denaturation of elastic fibers and non-enzymatic glycation may contribute to the mechanism of skin photo-aging.