中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2009年
6期
701-704
,共4页
短串联重复序列%遗传多态性%等位基因频率%湖北土家族
短串聯重複序列%遺傳多態性%等位基因頻率%湖北土傢族
단천련중복서렬%유전다태성%등위기인빈솔%호북토가족
short tandem repeat%genetic polymorphism%allele frequency%Hubei Tujia ethnic
目的 调查湖北地区土家族人群15个短串联重复序列(short tandem repeat,STR)基因座遗传多态性分布和群体遗传学数据,比较分析湖北土家族与重庆土家族等位基因的分布频率.方法 采用毛细管电泳技术和五色荧光复合扩增的方法,对湖北土家族333名无关个体的15个STR基因座进行基因分型.结果 共检出151个等位基因,多于重庆土家族的等位基因检出数(141个),其频率分布在0.002～0.498之间.经Statistical genetics软件分析,各基因座的群体基因型分布符合Hardy-Weinberg平衡(P＞0.05).15个STR基因座的杂合度在0.652～0.867之间,个体识别力在0.802～0.971之间,累积个体识别力大于0.9 999 999;非父排除率在0.357～0.730之间,累积非父排除率为0.9 999 997;多态信息含量在0.57～0.87之间.结论 建立了湖北土家族人群的STR基因座的群体资料,为土家族人群的群体遗传学研究、法医个人识别、亲子鉴定提供了基础数据;湖北土家族与重庆土家族亲缘关系相近,等位基因频率差异无统计学意义(P＞0.05),但在等位基因检出数及部分基因分布频率上仍存在差异.
目的 調查湖北地區土傢族人群15箇短串聯重複序列(short tandem repeat,STR)基因座遺傳多態性分佈和群體遺傳學數據,比較分析湖北土傢族與重慶土傢族等位基因的分佈頻率.方法 採用毛細管電泳技術和五色熒光複閤擴增的方法,對湖北土傢族333名無關箇體的15箇STR基因座進行基因分型.結果 共檢齣151箇等位基因,多于重慶土傢族的等位基因檢齣數(141箇),其頻率分佈在0.002～0.498之間.經Statistical genetics軟件分析,各基因座的群體基因型分佈符閤Hardy-Weinberg平衡(P＞0.05).15箇STR基因座的雜閤度在0.652～0.867之間,箇體識彆力在0.802～0.971之間,纍積箇體識彆力大于0.9 999 999;非父排除率在0.357～0.730之間,纍積非父排除率為0.9 999 997;多態信息含量在0.57～0.87之間.結論 建立瞭湖北土傢族人群的STR基因座的群體資料,為土傢族人群的群體遺傳學研究、法醫箇人識彆、親子鑒定提供瞭基礎數據;湖北土傢族與重慶土傢族親緣關繫相近,等位基因頻率差異無統計學意義(P＞0.05),但在等位基因檢齣數及部分基因分佈頻率上仍存在差異.
목적 조사호북지구토가족인군15개단천련중복서렬(short tandem repeat,STR)기인좌유전다태성분포화군체유전학수거,비교분석호북토가족여중경토가족등위기인적분포빈솔.방법 채용모세관전영기술화오색형광복합확증적방법,대호북토가족333명무관개체적15개STR기인좌진행기인분형.결과 공검출151개등위기인,다우중경토가족적등위기인검출수(141개),기빈솔분포재0.002～0.498지간.경Statistical genetics연건분석,각기인좌적군체기인형분포부합Hardy-Weinberg평형(P＞0.05).15개STR기인좌적잡합도재0.652～0.867지간,개체식별력재0.802～0.971지간,루적개체식별력대우0.9 999 999;비부배제솔재0.357～0.730지간,루적비부배제솔위0.9 999 997;다태신식함량재0.57～0.87지간.결론 건립료호북토가족인군적STR기인좌적군체자료,위토가족인군적군체유전학연구、법의개인식별、친자감정제공료기출수거;호북토가족여중경토가족친연관계상근,등위기인빈솔차이무통계학의의(P＞0.05),단재등위기인검출수급부분기인분포빈솔상잉존재차이.
Objective To investigate the genetic polymorphisms of 15 short tandem repeat(STR)loci in Tujia ethnic group of Hubei province,and to compare the allele frequencies between Hubei Tujia and Chongqing Tujia populations.MethodsCapillary electrophoresis and five-color fluorescent multiamplification were applied to detect the genotypes of 15 STR loci in 333 unrelated Hubei Tujia individuals.Results One hundred and fifty one alleles were observed in the population,which is higher than 141 alleles observed in Chongqin Tujia population,and their frequencies were from 0.002 to 0.498.No significant deviation from the Hardy-Weinberg Equilibrium was observed(P＞0.05).Statistical analysis showed that the heterozygosity of the 15 loci ranged from 0.652 to 0.867.The values of discrimination power(DP)ranged from 0.802 to 0.971.The values of excluding probability of paternity(EPP)ranged from 0.357 to 0.730,and the polymorphism information content(PIC)was 0.57-0.87.The fifteen loci showed an accumulated total discrimination power(TDP)of more than 0.999 999 9,and the cumulative excluding probability of paternity was 0.999 999 7.Conclusion This study has accumulated the population genetic data of the 15 STRs in Hubei Tujia ethnic group.The data can be used in forensic and individual identification for this population.Hubei Tujia is phylogenetically related to Chongqing Tujia,and the allele frequencies were non-distinctive(P＞0.05),while the number of alleles detected,and the frequencies of some alleles observed were different.
