CAJ | 학술논문

目的研究多发性骨髓瘤(multiple myeloma,MM)中lq21扩增情况,对比CD138磁珠分选(immunomagnetic cell sorting,MACS)结合间期荧光原位杂交(interphase fluorescence in situ hybridization,I-FISH)技术和胞浆轻链免疫荧光结合FISH (cytoplasmic light chain immunofluorescence-fluorescence in situ hybridization,cIg-FISH)技术的一致性,进一步探讨MM患者1q21扩增与疾病发展及预后之间的相关性.方法应用CD138 MACS结合1-FISH技术和cIg-FISH技术检测48例MM患者1q21扩增,比较两种检测方法的一致性,分析1q21扩增与疾病临床特征及预后的相关性.结果 CD138MACS结合I-FISH技术检测48例MM患者中31例(64.6％)出现了1q21的扩增.eIg-FISH技术检测48例MM患者中26例(54.2％)出现了1q21的扩增,两种检测方法阳性率差异无统计学意义(P＞0.05).1q21扩增阳性组患者死亡率明显高于阴性组(P＜0.05),但两组患者性别、年龄、Durie-Salmon分期、国际分期系统分期、疾病分组的差异无统计学意义(P＞0.05).结论 1q21扩增在MM中发生率较高,是MM不良的预后因素.cIg-FISH技术和CD138 MACS分选结合1-FISH技术一致性良好.
목적 연구다발성골수류(multiple myeloma,MM)중lq21확증정황,대비CD138자주분선(immunomagnetic cell sorting,MACS)결합간기형광원위잡교(interphase fluorescence in situ hybridization,I-FISH)기술화포장경련면역형광결합FISH (cytoplasmic light chain immunofluorescence-fluorescence in situ hybridization,cIg-FISH)기술적일치성,진일보탐토MM환자1q21확증여질병발전급예후지간적상관성.방법 응용CD138 MACS결합1-FISH기술화cIg-FISH기술검측48례MM환자1q21확증,비교량충검측방법적일치성,분석1q21확증여질병림상특정급예후적상관성.결과 CD138MACS결합I-FISH기술검측48례MM환자중31례(64.6％)출현료1q21적확증.eIg-FISH기술검측48례MM환자중26례(54.2％)출현료1q21적확증,량충검측방법양성솔차이무통계학의의(P＞0.05).1q21확증양성조환자사망솔명현고우음성조(P＜0.05),단량조환자성별、년령、Durie-Salmon분기、국제분기계통분기、질병분조적차이무통계학의의(P＞0.05).결론 1q21확증재MM중발생솔교고,시MM불량적예후인소.cIg-FISH기술화CD138 MACS분선결합1-FISH기술일치성량호.
Objective To investigate the prevlance of 1q21 amplification in patients with multiple myeloma (MM) and its correlation with the progression and prognosis of the disease. Method lq21 amplification was detected in 48 patients with MM using cytoplasmic light chain immunofluorescence with fluorescence in situ hybridization analysis (cIg-FISH) and interphase fluorescence in situ hybridization (IFISH) analysis combined with CD138 immunomagnetic cell sorting (MACS).Results 1q21 amplification (≥3 red signals) was detected in 26/48(54.2％)cases by cIg-FISH and 31/48 (64.6％) cases by I-FISH combined with CD138 MACS.There was a good consistency between the two methods (P＞0.05).The mortality of patients with 1 q21 amplification was significantly higher than those without (P＜0.05).No significant difference was detected in terms of sex,age,Durie-Salmon stage,subgroup and international staging system (ISS) stage between patients with 1q21 amplification and those without (P＞ 0.05).Conclusion The frequency of 1q21 amplification in MM is high.There was also an association between the amplification and poor prognosis.cIg-FISH is consistent with CD138 MACS combined with I-FISH.