中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2012年
5期
533-536
,共4页
王曦%张红军%胡永胜%宗振久%李英慧%李春义
王晞%張紅軍%鬍永勝%宗振久%李英慧%李春義
왕희%장홍군%호영성%종진구%리영혜%리춘의
肝细胞癌%SMURF1基因%基因表达%RNA干扰
肝細胞癌%SMURF1基因%基因錶達%RNA榦擾
간세포암%SMURF1기인%기인표체%RNA간우
Hepatocellular carcinoma%SMURF1 gene%Gene expression%RNA interference
目的 检测Smad泛素化调节因子1(Smad ubiquitination regulatory factor 1,SMURF1)基因在肝癌细胞中的表达水平,并探讨其在肝癌中的作用机制.方法 以相应的癌旁正常组织为对照,应用实时PCR和Western免疫印迹法检测89例肝细胞癌组织中SMURF1基因mRNA和蛋白表达的水平.分析基因表达水平与临床指标之间的相关性.在转染SMURF1特异性小干扰RNA(small interference RNA,siRNA)后,用流式细胞术及噻唑蓝比色法检测其对肝癌Hep G2细胞凋亡和增殖的影响.结果 肝癌组织中SMURF1 mRNA和蛋白表达水平均显著增高(P＜0.05).SMURF1表达水平与临床指标无明显的相关性(P＞0.05).转染SMURF1特异性siRNA可增加Hep G2细胞凋亡,抑制细胞增殖.结论 肝细胞癌中存在SMURF1基因的高表达,后者有可能影响肝癌细胞的凋亡及增殖,从而对于肝癌的发生和发展产生一定的影响.
目的 檢測Smad汎素化調節因子1(Smad ubiquitination regulatory factor 1,SMURF1)基因在肝癌細胞中的錶達水平,併探討其在肝癌中的作用機製.方法 以相應的癌徬正常組織為對照,應用實時PCR和Western免疫印跡法檢測89例肝細胞癌組織中SMURF1基因mRNA和蛋白錶達的水平.分析基因錶達水平與臨床指標之間的相關性.在轉染SMURF1特異性小榦擾RNA(small interference RNA,siRNA)後,用流式細胞術及噻唑藍比色法檢測其對肝癌Hep G2細胞凋亡和增殖的影響.結果 肝癌組織中SMURF1 mRNA和蛋白錶達水平均顯著增高(P＜0.05).SMURF1錶達水平與臨床指標無明顯的相關性(P＞0.05).轉染SMURF1特異性siRNA可增加Hep G2細胞凋亡,抑製細胞增殖.結論 肝細胞癌中存在SMURF1基因的高錶達,後者有可能影響肝癌細胞的凋亡及增殖,從而對于肝癌的髮生和髮展產生一定的影響.
목적 검측Smad범소화조절인자1(Smad ubiquitination regulatory factor 1,SMURF1)기인재간암세포중적표체수평,병탐토기재간암중적작용궤제.방법 이상응적암방정상조직위대조,응용실시PCR화Western면역인적법검측89례간세포암조직중SMURF1기인mRNA화단백표체적수평.분석기인표체수평여림상지표지간적상관성.재전염SMURF1특이성소간우RNA(small interference RNA,siRNA)후,용류식세포술급새서람비색법검측기대간암Hep G2세포조망화증식적영향.결과 간암조직중SMURF1 mRNA화단백표체수평균현저증고(P＜0.05).SMURF1표체수평여림상지표무명현적상관성(P＞0.05).전염SMURF1특이성siRNA가증가Hep G2세포조망,억제세포증식.결론 간세포암중존재SMURF1기인적고표체,후자유가능영향간암세포적조망급증식,종이대우간암적발생화발전산생일정적영향.
Objective To determine the expression level of Smad ubiquitination regulatory factor 1 (SMURF1) gene in hepatocellular carcinoma,and to explore its role in liver cancer.Methods With nonneoplastic adjacent normal tissues as controls,real-time PCR and Western blotting were used for measuring the expression of SMURF1 mRNA and protein in 89 samples of hepatocellular carcinoma.Correlations between SMURF1 expression and clinical features were explored.Following transfection of SMURF1-specific small interference RNA(siRNA),the apoptosis and proliferation of hepatic cancer cells Hep G2 were detected using flow cytometry and MTT assays.Results The expression of SMURF1 mRNA and protein were significantly higher in hepatocellular cancer tissues compared with the paired normal tissues (P＜0.05).The expression of SMURF1 however did not correlate with any clinical features (P＞0.05).Transfection of SMURF1-specific siRNA can promote the apoptosis whilst inhibit the proliferation of Hep G2 cells.Conclusion The expression of SMURF1 is enhanced in hepatocellular carcinoma,which may have played a role in the disease through affecting apoptosis and proliferation of hepatic cancer cells.
