中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2012年
5期
596-601
,共6页
蒲连美%南楠%杨泽%金泽宁
蒲連美%南楠%楊澤%金澤寧
포련미%남남%양택%금택저
SUMO4基因%多态性%冠状动脉粥样硬化性心脏病%2型糖尿病合并冠状动脉粥样硬化性心脏病
SUMO4基因%多態性%冠狀動脈粥樣硬化性心髒病%2型糖尿病閤併冠狀動脈粥樣硬化性心髒病
SUMO4기인%다태성%관상동맥죽양경화성심장병%2형당뇨병합병관상동맥죽양경화성심장병
SUMO4 gene%Polymorphisms%Coronary artery disease%Type 2 diabetes mellitus with coronary artery disease
目的 探讨北京地区汉族人群小泛素样修饰蛋白4 (small ubiquitin-like modifier 4,SUMO4)基因多态性与冠状动脉粥样硬化性心脏病(coronary artery disease,CAD)及2型糖尿病(type 2 diabetes mellitus,T2DM)合并CAD的关系.方法 采用病例对照设计,入选369例单纯CAD患者(CAD组)、189例2型糖尿病合并CAD患者(T2DM+ CAD组)及500名健康个体(对照组).应用聚合酶链反应-高分辨熔解曲线技术结合测序验证法,检测SUMO4基因rs237025、rs237024及rs600739 3个单核苷酸多态性(single nucleotide polymorphisms,SNPs)位点的基因型与等位基因在3组间的分布,并通过分层分析比较SNPs不同基因型携带者的临床相关危险因素的差异.结果 3个SNPs的等位基因和基因型在3组间的分布差异无统计学意义(P＞0.05)；通过分层分析,在T2DM+CAD组发现rs237025各基因型携带者的甘油三酯水平、rs600739各基因型携带者的体重指数水平的差异有统计学意义(P值分别为0.020和0.049),但组间多重比较仅发现rs237025的GG基因型携带者较GA及AA基因型携带者具有更高的甘油三酯水平(P＜0.01).结论 SUMO4基因多态性可能与北京地区汉族人群伴或不伴T2DM的CAD易感性无关.
目的 探討北京地區漢族人群小汎素樣脩飾蛋白4 (small ubiquitin-like modifier 4,SUMO4)基因多態性與冠狀動脈粥樣硬化性心髒病(coronary artery disease,CAD)及2型糖尿病(type 2 diabetes mellitus,T2DM)閤併CAD的關繫.方法 採用病例對照設計,入選369例單純CAD患者(CAD組)、189例2型糖尿病閤併CAD患者(T2DM+ CAD組)及500名健康箇體(對照組).應用聚閤酶鏈反應-高分辨鎔解麯線技術結閤測序驗證法,檢測SUMO4基因rs237025、rs237024及rs600739 3箇單覈苷痠多態性(single nucleotide polymorphisms,SNPs)位點的基因型與等位基因在3組間的分佈,併通過分層分析比較SNPs不同基因型攜帶者的臨床相關危險因素的差異.結果 3箇SNPs的等位基因和基因型在3組間的分佈差異無統計學意義(P＞0.05)；通過分層分析,在T2DM+CAD組髮現rs237025各基因型攜帶者的甘油三酯水平、rs600739各基因型攜帶者的體重指數水平的差異有統計學意義(P值分彆為0.020和0.049),但組間多重比較僅髮現rs237025的GG基因型攜帶者較GA及AA基因型攜帶者具有更高的甘油三酯水平(P＜0.01).結論 SUMO4基因多態性可能與北京地區漢族人群伴或不伴T2DM的CAD易感性無關.
목적 탐토북경지구한족인군소범소양수식단백4 (small ubiquitin-like modifier 4,SUMO4)기인다태성여관상동맥죽양경화성심장병(coronary artery disease,CAD)급2형당뇨병(type 2 diabetes mellitus,T2DM)합병CAD적관계.방법 채용병례대조설계,입선369례단순CAD환자(CAD조)、189례2형당뇨병합병CAD환자(T2DM+ CAD조)급500명건강개체(대조조).응용취합매련반응-고분변용해곡선기술결합측서험증법,검측SUMO4기인rs237025、rs237024급rs600739 3개단핵감산다태성(single nucleotide polymorphisms,SNPs)위점적기인형여등위기인재3조간적분포,병통과분층분석비교SNPs불동기인형휴대자적림상상관위험인소적차이.결과 3개SNPs적등위기인화기인형재3조간적분포차이무통계학의의(P＞0.05)；통과분층분석,재T2DM+CAD조발현rs237025각기인형휴대자적감유삼지수평、rs600739각기인형휴대자적체중지수수평적차이유통계학의의(P치분별위0.020화0.049),단조간다중비교부발현rs237025적GG기인형휴대자교GA급AA기인형휴대자구유경고적감유삼지수평(P＜0.01).결론 SUMO4기인다태성가능여북경지구한족인군반혹불반T2DM적CAD역감성무관.
Objective To assess the role of small ubiquitin-like modifier 4 (SUMO4)gene polymorphisms (rs237025,rs237024 and rs600739) in the susceptibility to coronary artery disease (CAD)with and without type 2 diabetes mellitus (T2DM) in Chinese Han ethnic population in Beijing.Methods In this case-control study,558 subjects with angiography-proven CAD were divided into two groups according to the WHO 1999 criteria:369 with normal glucose tolerance (CAD group) and 189 with T2DM (T2DM+CAD group).Meanwhile 500 healthy subjects free of T2DM and CAD were selected as normal controls (control group).Allelic and genotypic distributions of the three single nucleotide polymorphisms (SNPs) were determined with polymerase chain reaction-high resolution melting curve (PCR-HRM) and gene sequencing.Clinical and biochemical data were compared among carriers of different genotypes through a stratified analysis.Results No significant difference was found in the distribution of genotypes and alleles of each SNP between different groups (P＞0.05).Nevertheless,stratified analysis indicated a significant difference in plasma triglycerides (rs237025) and body mass index (rs600739) among individuals of different genotypes from the T2DM+CAD group (P=0.020 and P=0.049,respectively).Multiple comparison also indicated that GG genotype of rs237025 had a higher level of plasma triglycerides than AA genotype (P＜0.01).Conclusion No association between SUMO4 gene polymorphisms and CAD with and withoutT2DM was detected.Such polymorphisms may not be a risk factor for Chinese Han ethnic patients in Beijing.
