CAJ | 학술논문

目的研究主要精液参数和精子DNA完整率与习惯性流产的相关性.方法收集配偶具有习惯性流产史的患者85例,其精液标本与正常生育的男性(20名)进行对比研究.精子DNA检测采用精子染色质扩散试验法.结果在精液常规方面,精子活力与习惯性流产存在相关性(P＜0.05),习惯性流产组精子DNA断裂指数为(34.99±14.62)％,对照组为(10.82±4.80)％,两者比较差异具有统计学意义(P＜0.01).结论精子活力与习惯性流产存在相关性,习惯性流产组患者与正常生育组比较,具有较高精子DNA损伤.
목적 연구주요정액삼수화정자DNA완정솔여습관성유산적상관성.방법 수집배우구유습관성유산사적환자85례,기정액표본여정상생육적남성(20명)진행대비연구.정자DNA검측채용정자염색질확산시험법.결과 재정액상규방면,정자활력여습관성유산존재상관성(P＜0.05),습관성유산조정자DNA단렬지수위(34.99±14.62)％,대조조위(10.82±4.80)％,량자비교차이구유통계학의의(P＜0.01).결론 정자활력여습관성유산존재상관성,습관성유산조환자여정상생육조비교,구유교고정자DNA손상.
Objective To evaluate and compare standard sperm parameters and sperm DNA fragmentation in seminal ejaculates from men whose partners had a history of recurrent pregnancy loss (RPL) and a control group of men who had recently established their fertility.Methods Semen samples from 85 patients with a history of RPL and 20 men with proven fertility were analyzed according to World Health Organization guidelines.Sperm DNA fragmentation was detected by sperm chromatin dispersion test (SCD).Results A significant difference (P＜0.05) was observed in sperm motility but not other parameters between the two groups.The mean number of sperm cells with fragmented DNA,represented as DNA fragmentation index,was significantly increased in the RPL group [(34.99± 14.62)％] compared with controls [(10.82 ± 4.80) ％].Conclusion This study has indicated that sperm from men with a history of RPL have a higher incidence of DNA damage and poor motility compared with fertile males.