CAJ | 학술논문

目的探讨胎儿膀胱穿刺液细胞培养染色体分析的可行性.方法抽取3例超声检查诊断为巨大膀胱、羊水过少胎儿的膀胱内液体,取胎儿膀胱穿刺液细胞进行体外培养,染色体制备、核型分析.结果 3例的细胞培养均获得成功,获得了可供计数及核型分析的染色体分裂相.胎儿1的染色体核型为46,XY;胎儿2的染色体核型为46,XX,t(1；5)(q22; q12[7]/46,XX[4]；胎儿3的染色体核型为46,XY.结论胎儿膀胱穿刺液细胞经过体外培养可以用来进行染色体核型分析,使孕周较小、巨大膀胱、羊水过少胎儿的产前染色体检查成为可能,是产前诊断的一种新途径.
목적 탐토태인방광천자액세포배양염색체분석적가행성.방법 추취3례초성검사진단위거대방광、양수과소태인적방광내액체,취태인방광천자액세포진행체외배양,염색체제비、핵형분석.결과 3례적세포배양균획득성공,획득료가공계수급핵형분석적염색체분렬상.태인1적염색체핵형위46,XY;태인2적염색체핵형위46,XX,t(1；5)(q22; q12[7]/46,XX[4]；태인3적염색체핵형위46,XY.결론 태인방광천자액세포경과체외배양가이용래진행염색체핵형분석,사잉주교소、거대방광、양수과소태인적산전염색체검사성위가능,시산전진단적일충신도경.
Objective To explore the feasibility of karyotype analysis using cells cultured from fetal bladder centesis samples.Methods Samples were extracted upon fetal bladder centesis for 3 fetuses featuring giant bladder and oligohydramnios.Following in vitro culture,cells were routinely processed and stained for chromosome analysis.Results For all 3 cases,cell culture has achieved success.Sufficient metaphase cells were obtained for chromosome counting and karyotype analysis.The karyotypes of the 3 fetuses were respectively46,XY,46,XX,t(1;5)(q22;q12)[7]/46,XX[4],and 46,XY.Conclusion Cells cultured from fetal bladder centesis may be used for karyotype analysis following in vitro culturing.This new approach can enable prenatal chromosome analysis for fetuses featuring smaller gestational weeks,giant bladder and oligohydramnios.