中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2013年
3期
357-361
,共5页
费前进%金建远%倪吴花%黄学锋
費前進%金建遠%倪吳花%黃學鋒
비전진%금건원%예오화%황학봉
精子%不育%DNA碎片指数%变异
精子%不育%DNA碎片指數%變異
정자%불육%DNA쇄편지수%변이
Sperm%Infertility%DNA fragmentation index%Variation
目的 探讨不育夫妇中男性精子DNA碎片指数(DNA fragmentation index,DFI)的变异程度.方法 2009年4月至2012年4月,将539对不育夫妇中的539例男性纳入本研究.根据世界卫生组织标准(1999年),应用精子染色质扩散(sperm chromatin dispersion,SCD)试验对每名男性进行至少1次的重复精液常规分析和DFI检测,计算DFI的变异系数(coefficient of variation,CV),对DFI的变异程度及其影响因素进行统计分析.结果 539例男性中,有473例、59例、6例和1例分别行1次、2次、3次和4次重复精液常规分析和DFI检测,共计613次重复检测,重复检测和首次检测的间隔时间为0.5～34个月,中位数3.0(1.0～11.0)个月.539份首次检测样本间的DFI变异系数(between-sample coefficient of variation,CVB)为71.2％.与首次检测间隔0.5～3个月、3～12个月和12～34个月的DFI与首次检测的DFI比较,差异均有统计学意义(P＜0.01).以18％作为男性生育力的DFI阈值,539例男性中有142例(26.3％,95％CI:22.6％～30.0％)的首次和重复检测DFI值分别位于阈值两侧.539例男性自身DFI的变异系数(within-subject coefficient of variation,CVw)为0.0～110.5％,中位数为26.0％(12.6％～42.8％),精子总数、精子浓度、精子活动力和精子形态的CVw的中位数分别为41.9％(21.8％～72.6％)、31.9％(15.1％～62.5％)、29.4％(13.2％～55.4％)和46.0％(22.4％～87.1％),各精液常规参数的CVw分别与DFI的CVw比较,差异均有统计学意义(P＜0.01).DFI的CVw与精子总数、精子浓度和DFI重复检测的间隔时间密切相关(P＜0.05).结论 不育夫妇中的男性精子DFI是一个变异较大的参数,应对每例男性的DFI进行重复检测才能反映其精子DNA损伤的水平.
目的 探討不育伕婦中男性精子DNA碎片指數(DNA fragmentation index,DFI)的變異程度.方法 2009年4月至2012年4月,將539對不育伕婦中的539例男性納入本研究.根據世界衛生組織標準(1999年),應用精子染色質擴散(sperm chromatin dispersion,SCD)試驗對每名男性進行至少1次的重複精液常規分析和DFI檢測,計算DFI的變異繫數(coefficient of variation,CV),對DFI的變異程度及其影響因素進行統計分析.結果 539例男性中,有473例、59例、6例和1例分彆行1次、2次、3次和4次重複精液常規分析和DFI檢測,共計613次重複檢測,重複檢測和首次檢測的間隔時間為0.5～34箇月,中位數3.0(1.0～11.0)箇月.539份首次檢測樣本間的DFI變異繫數(between-sample coefficient of variation,CVB)為71.2％.與首次檢測間隔0.5～3箇月、3～12箇月和12～34箇月的DFI與首次檢測的DFI比較,差異均有統計學意義(P＜0.01).以18％作為男性生育力的DFI閾值,539例男性中有142例(26.3％,95％CI:22.6％～30.0％)的首次和重複檢測DFI值分彆位于閾值兩側.539例男性自身DFI的變異繫數(within-subject coefficient of variation,CVw)為0.0～110.5％,中位數為26.0％(12.6％～42.8％),精子總數、精子濃度、精子活動力和精子形態的CVw的中位數分彆為41.9％(21.8％～72.6％)、31.9％(15.1％～62.5％)、29.4％(13.2％～55.4％)和46.0％(22.4％～87.1％),各精液常規參數的CVw分彆與DFI的CVw比較,差異均有統計學意義(P＜0.01).DFI的CVw與精子總數、精子濃度和DFI重複檢測的間隔時間密切相關(P＜0.05).結論 不育伕婦中的男性精子DFI是一箇變異較大的參數,應對每例男性的DFI進行重複檢測纔能反映其精子DNA損傷的水平.
목적 탐토불육부부중남성정자DNA쇄편지수(DNA fragmentation index,DFI)적변이정도.방법 2009년4월지2012년4월,장539대불육부부중적539례남성납입본연구.근거세계위생조직표준(1999년),응용정자염색질확산(sperm chromatin dispersion,SCD)시험대매명남성진행지소1차적중복정액상규분석화DFI검측,계산DFI적변이계수(coefficient of variation,CV),대DFI적변이정도급기영향인소진행통계분석.결과 539례남성중,유473례、59례、6례화1례분별행1차、2차、3차화4차중복정액상규분석화DFI검측,공계613차중복검측,중복검측화수차검측적간격시간위0.5～34개월,중위수3.0(1.0～11.0)개월.539빈수차검측양본간적DFI변이계수(between-sample coefficient of variation,CVB)위71.2％.여수차검측간격0.5～3개월、3～12개월화12～34개월적DFI여수차검측적DFI비교,차이균유통계학의의(P＜0.01).이18％작위남성생육력적DFI역치,539례남성중유142례(26.3％,95％CI:22.6％～30.0％)적수차화중복검측DFI치분별위우역치량측.539례남성자신DFI적변이계수(within-subject coefficient of variation,CVw)위0.0～110.5％,중위수위26.0％(12.6％～42.8％),정자총수、정자농도、정자활동력화정자형태적CVw적중위수분별위41.9％(21.8％～72.6％)、31.9％(15.1％～62.5％)、29.4％(13.2％～55.4％)화46.0％(22.4％～87.1％),각정액상규삼수적CVw분별여DFI적CVw비교,차이균유통계학의의(P＜0.01).DFI적CVw여정자총수、정자농도화DFI중복검측적간격시간밀절상관(P＜0.05).결론 불육부부중적남성정자DFI시일개변이교대적삼수,응대매례남성적DFI진행중복검측재능반영기정자DNA손상적수평.
Objective To investigate variation of sperm DNA fragmentation index (DFI) in male partners of infertile couples.Methods A total of 539 males between April 2009 and April 2012 were analyzed.At least one repeated routine semen analysis and sperm DNA fragmentation test were performed for each sample by sperm chromatin dispersion (SCD) analysis following World Health Organization guidelines.Coefficient of variation (CV) for DFI was calculated.Results Respectively,1,2,3 and 4 repeated SCD analyses were carried out on 473,59,6 and 1 semen samples.The median interval between the first and repeated SCD measurements was 3.0 (1.0-11.0) months.For the first tested samples,the between-sample coefficient of variation (CVB) for DFI was 71.2 ％.A significant difference had been found between DFI of the first measurement and DFI of repeated measurement in 0.5 to 3 months,3 to 12 months and 12 to 34 months (P＜0.01).Compared with the first test,26.3％ of males were on both sides of the cut-off point of 18％.The median within-subject coefficient of variation (CVw) for DFI of 539 men was 26.0％ (12.6 ％-42.8 ％).And the median CVw DFI was significantly lower compared with CVw of sperm count,concentration,progressive motility and normal morphology (P＜0.01).Significant correlations were found between the CVw DFI and sperm count,concentration and interval among the samples (P＜0.05).Conclusion DFI of male partners for infertile couples is a parameter with substantial variation,repeated SCD measurements are therefore recommended.
