中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2014年
1期
93-96
,共4页
余金玲%姚津剑%里进%安会敏%陈曼%宋起龙%林菊生
餘金玲%姚津劍%裏進%安會敏%陳曼%宋起龍%林菊生
여금령%요진검%리진%안회민%진만%송기룡%림국생
乙型重型肝炎%肝硬化%肝癌%转归%单核苷酸多态性%HLA-DQA1基因
乙型重型肝炎%肝硬化%肝癌%轉歸%單覈苷痠多態性%HLA-DQA1基因
을형중형간염%간경화%간암%전귀%단핵감산다태성%HLA-DQA1기인
Acute liver failure%Hepatic cirrhosis%Liver cancer%Clinic outcome%Single nucleotide polymorphism%HLA-DQA1 gene
目的 探讨中国湖北地区人类白细胞抗原(human leukocyte antigen,HLA-DQA1)基因rs9272346单核苷酸多态性与慢性乙型肝炎不同转归的关联.方法 采用病例-对照研究,招募湖北地区汉族35岁以上住院乙型重型肝炎(acute liver failure,ALF)173例、乙肝相关性肝硬化(liver cirrhosis,LC)292例、乙肝相关性肝癌(hepatocellular carcinoma,HCC) 325例、无症状乙肝携带者(asymptomatic HBV carrier,AHC)238例.应用TaqMan MGB探针实时PCR技术检测rs9272346位点基因型,应用T检验、卡方检验和非条件Logistic回归进行统计分析.结果 G等位基因型在ALF、LC、HCC组与AHC组分布差异无统计学意义(P=0.312、0.314、0.264).在显性模型下,校正性别年龄因素后,GG+GA与AA基因型相比,与ALF(OR=1.08,95％CI:0.70～1.68)、LC(OR=1.11,95％CI:0.87～1.26)、HCC(OR=0.93,95％CI:0.65～1.33)患病风险无相关性.性别分层后显性模型下显示的女性患者中,GG+ GA基因型与AA基因型相比为进展为重型肝炎的保护因素(OR=0.30,95 ％CI:0.1～0.87).结论 HLA-DQA1基因rs9272346位点单核苷酸多态性与乙型肝炎不同转归无相关性,女性患者中G等位基因携带可能是进展为重型肝炎的保护因素.
目的 探討中國湖北地區人類白細胞抗原(human leukocyte antigen,HLA-DQA1)基因rs9272346單覈苷痠多態性與慢性乙型肝炎不同轉歸的關聯.方法 採用病例-對照研究,招募湖北地區漢族35歲以上住院乙型重型肝炎(acute liver failure,ALF)173例、乙肝相關性肝硬化(liver cirrhosis,LC)292例、乙肝相關性肝癌(hepatocellular carcinoma,HCC) 325例、無癥狀乙肝攜帶者(asymptomatic HBV carrier,AHC)238例.應用TaqMan MGB探針實時PCR技術檢測rs9272346位點基因型,應用T檢驗、卡方檢驗和非條件Logistic迴歸進行統計分析.結果 G等位基因型在ALF、LC、HCC組與AHC組分佈差異無統計學意義(P=0.312、0.314、0.264).在顯性模型下,校正性彆年齡因素後,GG+GA與AA基因型相比,與ALF(OR=1.08,95％CI:0.70～1.68)、LC(OR=1.11,95％CI:0.87～1.26)、HCC(OR=0.93,95％CI:0.65～1.33)患病風險無相關性.性彆分層後顯性模型下顯示的女性患者中,GG+ GA基因型與AA基因型相比為進展為重型肝炎的保護因素(OR=0.30,95 ％CI:0.1～0.87).結論 HLA-DQA1基因rs9272346位點單覈苷痠多態性與乙型肝炎不同轉歸無相關性,女性患者中G等位基因攜帶可能是進展為重型肝炎的保護因素.
목적 탐토중국호북지구인류백세포항원(human leukocyte antigen,HLA-DQA1)기인rs9272346단핵감산다태성여만성을형간염불동전귀적관련.방법 채용병례-대조연구,초모호북지구한족35세이상주원을형중형간염(acute liver failure,ALF)173례、을간상관성간경화(liver cirrhosis,LC)292례、을간상관성간암(hepatocellular carcinoma,HCC) 325례、무증상을간휴대자(asymptomatic HBV carrier,AHC)238례.응용TaqMan MGB탐침실시PCR기술검측rs9272346위점기인형,응용T검험、잡방검험화비조건Logistic회귀진행통계분석.결과 G등위기인형재ALF、LC、HCC조여AHC조분포차이무통계학의의(P=0.312、0.314、0.264).재현성모형하,교정성별년령인소후,GG+GA여AA기인형상비,여ALF(OR=1.08,95％CI:0.70～1.68)、LC(OR=1.11,95％CI:0.87～1.26)、HCC(OR=0.93,95％CI:0.65～1.33)환병풍험무상관성.성별분층후현성모형하현시적녀성환자중,GG+ GA기인형여AA기인형상비위진전위중형간염적보호인소(OR=0.30,95 ％CI:0.1～0.87).결론 HLA-DQA1기인rs9272346위점단핵감산다태성여을형간염불동전귀무상관성,녀성환자중G등위기인휴대가능시진전위중형간염적보호인소.
Objective To assess the association of rs9272346 polymorphism of HLA-DQA1 gene with clinical outcome of hepatitis B virus (HBV) infection in ethnic Han population from Hubei,China.Method A case-control study was conducted,which have involved 1028 unrelated subjects including 238asymptomatic HBV carriers (AHC),173 acute liver failure (ALF),292 liver cirrhosis (LC) and 325hepatocellular carcinoma (HCC).Genotypes of rs9272346 were determined by real-time polymerase chain reaction with a TaqMan MGB probe.Statistical results were analyzed using Chi square test,student's t test and unconditional,logistic regression.Results No significant differences were detected in the frequencies of G allele between ALF,LC,HCC and AHC groups (P=0.312,0.314,0.264).Compared with the AA genotype,the GG and GA genotypes were not associated with the patients groups under the dominant model:ALF group vs.AHC group (adjusted OR=1.08,95％CI:0.7-1.68),LC group vs.AHC group (adjusted OR=1.11,95％CI:0.87-1.26),HCC group vs.AHC group (adjusted OR=0.93,95％CI:0.65-1.33).For women,the GG and GA genotypes have conferred a protective effect for the outcome of ALF (OR=0.30,95％CI:0.1-1.87).Conclusion Our results suggested that rs9272346 polymorphism of HLA-DQA1 may not independently influence the outcome of HBV infection in ethnic Han Chinese in Hubei,while the GG and GA genotypes may confer a protective effect against ALF in women.
