CAJ | 학술논문

目的通过分子生物学方法,筛选广西壮族随机献血者样本中的Fy(a-)、s-、k-、Di(b-)、Js(b-)表型,获得壮族稀有血型资料,丰富中国稀有血型库.方法基于血型抗原Fyb、s等位基因单核苷酸多态性位点,设计序列特异性引物,优化PCR条件,建立多重PCR体系Ⅰ;并使用筛选稀有血型等位基因Dib、k、Jsb1910、Jsb2019多重PCR体系Ⅱ共同对4490份广西壮族随机献血者样本进行筛选.结果成功构建检测血型抗原Fyb、s的多重PCR体系Ⅰ,经验证多重PCR体系Ⅰ具有良好的准确度和稳定性.使用多重PCR体系Ⅰ和Ⅱ在4490份广西壮族随机献血者样本中共检出5例Fy(a-),3例s(-)及2例Di(b-).结论多重PCR能够用于稀有血型的大规模筛选,获得的广西壮族人群稀有血型样本丰富了稀有血型数据库,为临床输注配合性血液提供了宝贵资料.
목적 통과분자생물학방법,사선엄서장족수궤헌혈자양본중적Fy(a-)、s-、k-、Di(b-)、Js(b-)표형,획득장족희유혈형자료,봉부중국희유혈형고.방법 기우혈형항원Fyb、s등위기인단핵감산다태성위점,설계서렬특이성인물,우화PCR조건,건립다중PCR체계Ⅰ;병사용사선희유혈형등위기인Dib、k、Jsb1910、Jsb2019다중PCR체계Ⅱ공동대4490빈엄서장족수궤헌혈자양본진행사선.결과 성공구건검측혈형항원Fyb、s적다중PCR체계Ⅰ,경험증다중PCR체계Ⅰ구유량호적준학도화은정성.사용다중PCR체계Ⅰ화Ⅱ재4490빈엄서장족수궤헌혈자양본중공검출5례Fy(a-),3례s(-)급2례Di(b-).결론 다중PCR능구용우희유혈형적대규모사선,획득적엄서장족인군희유혈형양본봉부료희유혈형수거고,위림상수주배합성혈액제공료보귀자료.
Objective To screen rare blood groups Fy(a-),s-,k-,Di(b-) and Js(b-) in an ethnic Zhuang population.Methods Sequence-specific primers were designed based on single nucleotide polymorphism (SNP) sites of blood group antigens Fyb and s.A specific multiplex PCR system Ⅰ was established.Multiplex PCR system Ⅱ was applied to detect alleles antigens Dib,k,Jsb1910 and Jsb2019 at the same time.The two systems was were used to screen for rare blood group antigens in 4490 randomly selected healthy donors of Guangxi Zhuang ethnic origin.Results We successfully made the multiplex PCR system Ⅰ.We detected the rare blood group antigens using the two PCR system.There are five Fy(a-),three s(-),two Di(b-) in 4490 Guangxi zhuang random samples.The multiplex PCR system Ⅰ has achieved good accuracy and stability.With multiplex PCR systems Ⅰ and Ⅱ,4490 samples were screened.Five Fy (a-),three s(-) and two Di(b-) samples were discovered.Conclusion Multiplex PCR is an effective methods,which can be used for high high-throughput screening of rare blood groups.The rare blood types of Guangxi Zhuang ethnic origin obtained through the screening can provide valuable information for compatible blood transfusion.Through screening we obtained precious rare blood type materials which can be used to improve the capability of compatible infusion and reduce the transfusion reactions.