中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2012年
11期
826-830
,共5页
陈学忠%张洁瑜%曾家豫%杨荣%刘芳%赵静%张渭波%王宇仙%毛爱红%唐文茹%廖世奇
陳學忠%張潔瑜%曾傢豫%楊榮%劉芳%趙靜%張渭波%王宇仙%毛愛紅%唐文茹%廖世奇
진학충%장길유%증가예%양영%류방%조정%장위파%왕우선%모애홍%당문여%료세기
骨髓闻充质干细胞%脂细胞%诱导%细胞分化%大鼠
骨髓聞充質榦細胞%脂細胞%誘導%細胞分化%大鼠
골수문충질간세포%지세포%유도%세포분화%대서
Bone marrow mesenchymal stem cells%Adipocytes%Induction%Cell differentiation%Rat
目的 通过阻断正常干细胞的分化过程,探讨肿瘤发生机制.方法 利用贴壁培养法获得大鼠骨髓间充质干细胞(BMSCs),通过地塞米松、胰岛素和吲哚美辛联合诱导大鼠BMSCs,使其分化为脂肪细胞.在细胞分化过程中利用3-甲基胆蒽(3-MC)阻断大鼠BMSCs的分化过程.结果 大鼠BMSCs分化实验结果显示,诱导30 d后,经油红O染色,细胞内可见脂肪细胞特有的红色脂滴沉淀,且随着诱导时间的延长,油红O阳性细胞比例增加,脂滴增大,表明大鼠BMSCs已成功分化为脂肪细胞.大鼠BMSCs分化阻断实验结果显示,诱导30 d后,经油红O染色,细胞内红色脂滴沉淀明显下降,表明阻断后的细胞具有脂肪细胞的特性,但分化不完全；形态学鉴定表现为细胞接触抑制消失、细胞核异常、微核畸变率高和核型异常,表明阻断后的干细胞发生恶性转变.结论 大鼠BMSCs分化过程受到阻断,细胞停止在分化的中间阶段,有转化为肿瘤细胞的趋势.
目的 通過阻斷正常榦細胞的分化過程,探討腫瘤髮生機製.方法 利用貼壁培養法穫得大鼠骨髓間充質榦細胞(BMSCs),通過地塞米鬆、胰島素和吲哚美辛聯閤誘導大鼠BMSCs,使其分化為脂肪細胞.在細胞分化過程中利用3-甲基膽蒽(3-MC)阻斷大鼠BMSCs的分化過程.結果 大鼠BMSCs分化實驗結果顯示,誘導30 d後,經油紅O染色,細胞內可見脂肪細胞特有的紅色脂滴沉澱,且隨著誘導時間的延長,油紅O暘性細胞比例增加,脂滴增大,錶明大鼠BMSCs已成功分化為脂肪細胞.大鼠BMSCs分化阻斷實驗結果顯示,誘導30 d後,經油紅O染色,細胞內紅色脂滴沉澱明顯下降,錶明阻斷後的細胞具有脂肪細胞的特性,但分化不完全；形態學鑒定錶現為細胞接觸抑製消失、細胞覈異常、微覈畸變率高和覈型異常,錶明阻斷後的榦細胞髮生噁性轉變.結論 大鼠BMSCs分化過程受到阻斷,細胞停止在分化的中間階段,有轉化為腫瘤細胞的趨勢.
목적 통과조단정상간세포적분화과정,탐토종류발생궤제.방법 이용첩벽배양법획득대서골수간충질간세포(BMSCs),통과지새미송、이도소화신타미신연합유도대서BMSCs,사기분화위지방세포.재세포분화과정중이용3-갑기담은(3-MC)조단대서BMSCs적분화과정.결과 대서BMSCs분화실험결과현시,유도30 d후,경유홍O염색,세포내가견지방세포특유적홍색지적침정,차수착유도시간적연장,유홍O양성세포비례증가,지적증대,표명대서BMSCs이성공분화위지방세포.대서BMSCs분화조단실험결과현시,유도30 d후,경유홍O염색,세포내홍색지적침정명현하강,표명조단후적세포구유지방세포적특성,단분화불완전；형태학감정표현위세포접촉억제소실、세포핵이상、미핵기변솔고화핵형이상,표명조단후적간세포발생악성전변.결론 대서BMSCs분화과정수도조단,세포정지재분화적중간계단,유전화위종류세포적추세.
Objective To explore the pathogenesis of tumors by blocking the normal differentiation process of stem cells.Methods Bone marrow mesenchymal stem cells (BMSCs) from rats were isolated,cultured and purified by whole bone marrow adherence method.The rat BMSCs were induced to differentiate into adipocytes with dexamethasone,insulin and indomethacin.Blockage of the differentiation process was induced by 3-methylcholanthrene (3-MC).Results The differentiation experiment showed that at 30 days after the induction,oil red O staining-positive cells occurred with increased intracytolasmic lipid droplets,characteristic for adipocytes.The differentiation blockage experiment showed that at 30 days after induction,the deposits of oil red O staining-cytoplasmic lipid droplets was significantly reduced,indicating that the blocked cells were adipocytes,but not fully differentiated.Morphological identification showed that cell contact inhibition disappeared,abnormal cell nuclei,increased number of micronucleus aberration and karyotype abnormalities,indicating that malignant transformation of the stem cells occurred after the differentiation blockage.Conclusions The results of this study show a blockage of the differentiation of that stem cells at the intermediate phase,and a tendency of malignant transformation of the stem cells.The results of our study provide new evidence that cancer stem cells may be originated by suppression of stem cell differentiation.
