CAJ | 학술논문

目的探讨SET8基因3'非翻译区单核苷酸多态性(SNP) rs16917496对乳腺癌发病年龄的影响.方法选取1110例乳腺癌患者,采用聚合酶链反应-限制性片段长度多态性法检测患者的基因型,采用SAS软件分析不同基因型或基因型组合的乳腺癌患者发病年龄趋势.结果 SET8基因CC基因型和TP53基因GG基因型均可以使乳腺癌发病年龄提前,而且存在等位基因数量-反应关系.同时具有SET8 CC基因型和TP53基因型的患者平均发病年龄为47.74岁,而同时具有SET8 TT和TP53 CC基因型的患者平均发病年龄为54.55岁.结论 SET8基因miR-502靶序列SNP与乳腺癌发病年龄有关,且与TP53密码子72 SNP存在联合作用.
목적 탐토SET8기인3'비번역구단핵감산다태성(SNP) rs16917496대유선암발병년령적영향.방법 선취1110례유선암환자,채용취합매련반응-한제성편단장도다태성법검측환자적기인형,채용SAS연건분석불동기인형혹기인형조합적유선암환자발병년령추세.결과 SET8기인CC기인형화TP53기인GG기인형균가이사유선암발병년령제전,이차존재등위기인수량-반응관계.동시구유SET8 CC기인형화TP53기인형적환자평균발병년령위47.74세,이동시구유SET8 TT화TP53 CC기인형적환자평균발병년령위54.55세.결론 SET8기인miR-502파서렬SNP여유선암발병년령유관,차여TP53밀마자72 SNP존재연합작용.
Objective We have identified a SNP within the seed-binding region for miR-502 in the 3'-UTR of the SET8 gene that codes for a methyltransferase for histone H4.SET8 methylates TP53 and thus regulates cell proliferation and genome stability.This study is to investigate the role for this SNP and its interaction with the TP53 codon 72 SNP in the age of onset of breast cancer.Methods We conducted a case-only study of 1,110 breast cancer cases.PCR-RFLP was used for SNP genotyping.Ages of onset of breast cancer among different genotypes were analyzed using SAS software.Results Our analysis revealed that the SET8 CC and TP53 GG genotypes were independently associated with earlier age of onset of breast cancer in an allele-dose dependent manner.Moreover,individuals with both SET8 CC and p53 GG genotypes developed cancer at age of 47.74 years,compared with 54.55 years for individuals with both SET8 TT and TP53 CC genotypes.Conclusions miR-502-binding SNP in SET8 may modulate SET8 expression and contribute to early development of breast cancer either independently.or together with the TP53 codon 72 SNP.