CAJ | 학술논문

目的观察5-氟尿嘧啶(5-Fu)对人纤维肉瘤HT-1080细胞体外增殖的抑制作用.方法取处于对数生长期的人纤维肉瘤HT-1080细胞,分别加入不同浓度的阿霉素和5-Fu培养,于培养24、72和144h时,采用四甲基偶氮唑蓝(MTT)法测定HT-1080细胞的吸光度(A)并计算细胞增殖抑制率,倒置相差显微镜下观察细胞形态的变化,流式细胞仪检测细胞周期.结果 10 μg/ml 5-Fu处理人纤维肉瘤HT-1080细胞24、72和144 h时,HT-1080细胞的增殖抑制率分别为45.9％、64.7％和90.6％;100 μg/ml 5-Fu处理人纤维肉瘤HT-1080细胞24、72和144 h时,HT-1080细胞的增殖抑制率分别为53.1％、86.4％和93.0％,5-Fu显示出与阿霉素相似的细胞毒作用.经不同浓度的阿霉素和高浓度5-Fu处理后,HT-1080细胞可表现出明显的凋亡特征.流式细胞仪检测结果显示,未处理的HT-1080细胞中,G1期细胞占67.5％,S期细胞占21.2％,G2期细胞占11.3％.随着5-Fu浓度的增加,G1+S期细胞的比例逐渐增加,当5-Fu的浓度≥1μg/ml时,未再探及G2期细胞.结论 5-Fu在体外对人纤维肉瘤HT-1080细胞的增殖有明显的抑制作用,且呈时间和浓度依赖性;5-Fu在高浓度连续给药后表现出与阿霉素类似的抑瘤效果,能将HT-1080细胞阻滞于G1+S期.
목적 관찰5-불뇨밀정(5-Fu)대인섬유육류HT-1080세포체외증식적억제작용.방법 취처우대수생장기적인섬유육류HT-1080세포,분별가입불동농도적아매소화5-Fu배양,우배양24、72화144h시,채용사갑기우담서람(MTT)법측정HT-1080세포적흡광도(A)병계산세포증식억제솔,도치상차현미경하관찰세포형태적변화,류식세포의검측세포주기.결과 10 μg/ml 5-Fu처리인섬유육류HT-1080세포24、72화144 h시,HT-1080세포적증식억제솔분별위45.9％、64.7％화90.6％;100 μg/ml 5-Fu처리인섬유육류HT-1080세포24、72화144 h시,HT-1080세포적증식억제솔분별위53.1％、86.4％화93.0％,5-Fu현시출여아매소상사적세포독작용.경불동농도적아매소화고농도5-Fu처리후,HT-1080세포가표현출명현적조망특정.류식세포의검측결과현시,미처리적HT-1080세포중,G1기세포점67.5％,S기세포점21.2％,G2기세포점11.3％.수착5-Fu농도적증가,G1+S기세포적비례축점증가,당5-Fu적농도≥1μg/ml시,미재탐급G2기세포.결론 5-Fu재체외대인섬유육류HT-1080세포적증식유명현적억제작용,차정시간화농도의뢰성;5-Fu재고농도련속급약후표현출여아매소유사적억류효과,능장HT-1080세포조체우G1+S기.
Objective To evaluate the inhibitory effect of 5-fluorouracil (5-Fu) on HT-1080 human fibrosarcoma cells in vitro.Methods HT-1080 human fibrosarcoma cells were cultured for 3 days in the proliferation period.Then adriamycin or 5-Fu at different concentrations were used to treat these cells for 24 h,72 h and 144 h.MTT assay was used to evaluate the cytotoxic effects through measuring optical density and calculating the inhibition rate of cell growth.Morphologic changes of the cells were observed under a phase contrast microscope.Flow cytometry (FCM) was performed to detect the changes in cell cycle and DNA ploidy in the fibrosarcoma cells treated with 5-Fu.Results 10 μg/ml 5-Fu showed an inhibition rate of 45.9％ (24 h),64.7％ (72 h) and 90.6％ (144 h) of the HT-1080 cell growth.100 μg/ml 5-Fu showed an inhibition rate of 53.1％ (24 h),86.4％ (72 h),93.0％ (144 h) of the HT-1080 cell growth,results similar to those in the adriamycin group.Untreated fibrosarcoma cells accounted for 67.5％ in G1 phase,21.2％ in S phase and 11.3％ in G2 phase.With the increasing drug concentrations,cells in G1 +S phase increased rapidly and no cells in G2 phase were observed later.The cells treated with 5-Fu showed a G1 + S cell cycle arrest.Conclusions 5-Fu has an antitumor activity in human fibrosarcoma HT-1080 cells in vitro,in a time-dependent and dose-dependent manner.The cytotoxity of 5-Fu at high concentrations and continuous use can induce tumor cell cycle arrested at G1 + S phase,a similar result induced with adriamycin.